RAB4A / RAB4 antibody

Principal name

RAB4A / RAB4 antibody

Alternative names for RAB4A / RAB4 antibody

Rab-4A, Ras-related protein Rab-4A

SwissProt ID

P05714 (Rat), P20338 (Human), P56371 (Mouse), Q2TBH7 (Bovin), Q6PHI9 (Danre)

Gene ID

5867 (RAB4A), 25532 (Rab4a)

Available reactivities

Hu (Human), Rt (Rat), Mky (Monkey), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of RAB4A / RAB4 antibody

Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound ictive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) . Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment . Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COOH-termil domains with a cysteine motif implicated in sub-cellular targeting. Post-translatiol modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins . Each Rab shows a characteristic sub-cellular distribution . In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin . Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-termil cdc2 kise consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol .

General readings

Li L. et al. (2001) J Biol Chem. 276:5265-73.
Lindsay AJ.. et al. (2002) J Biol Chem. 277:12190-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAB4A / RAB4 antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RAB4A mouse mAb with DAB staining. Mouse IgG1 4E11 Purified Hu, Mky, Ms E, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RAB4A / RAB4 antibody

  Rabbit polyclonal Rab4 Antibody  
Western blot analysis of Rab4 in HeLa cell lysates using a 1:1000 dilution of the antibody Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAB4A / RAB4 antibody

  Rabbit Polyclonal Anti-RAB4A Antibody  
WB Suggested Anti-RAB4A Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAB4A / RAB4 antibody

  Mouse Monoclonal RAB4A Antibody  
Western blot detection of RAB4A in Hela,PC-12,COS7 and mouse brain cell lysates using RAB4A mouse mAb (1:500 diluted).Predicted band size:24KDa.Observed band size:24KDa. Mouse IgG2a 3D1-A9-H10 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

RAB4A - human gene knockout kit via CRISPR

RAB4A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RAB4A - human gene knockout kit via CRISPR

RAB4A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RAB4A - human gene knockout kit via CRISPR

RAB4A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RAB4A - human gene knockout kit via CRISPR

RAB4A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAB4A / RAB4

  Recombinant protein of human RAB4A, member RAS oncogene family (RAB4A)  
RAB4A / RAB4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAB4A / RAB4 (1-218, His-tag)

Recombinant human RAB4A, 1-218aa, His-tagged Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

RAB4A / RAB4 (1-218, His-tag)

Recombinant human RAB4A, 1-218aa, His-tagged Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates (Knockout)

Catalog No.    

RAB4A-knockout Cell lysate

RAB4A-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RAB4A (mGFP-tagged) - Human RAB4A, member RAS oncogene family (RAB4A), 200 uL, >10^7 TU/mL

Lenti ORF particles, RAB4A (mGFP-tagged) - Human RAB4A, member RAS oncogene family (RAB4A), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, RAB4A (Myc-DDK tagged) - Human RAB4A, member RAS oncogene family (RAB4A) , 200 uL, >10^7 TU/mL

Lenti ORF particles, RAB4A (Myc-DDK tagged) - Human RAB4A, member RAS oncogene family (RAB4A) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rab4a (GFP-tagged ORF) - Rat RAB4A, member RAS oncogene family (Rab4a), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rab4a (GFP-tagged ORF) - Rat RAB4A, member RAS oncogene family (Rab4a), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rab4a (Myc-DDK-tagged ORF) - Rat RAB4A, member RAS oncogene family (Rab4a), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rab4a (Myc-DDK-tagged ORF) - Rat RAB4A, member RAS oncogene family (Rab4a), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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