RAB4A / RAB4 antibody

Principal name

RAB4A / RAB4 antibody

Alternative names for RAB4A / RAB4 antibody

Rab-4A, Ras-related protein Rab-4A

SwissProt ID

P05714 (Rat), P20338 (Human), P56371 (Mouse), Q2TBH7 (Bovin), Q6PHI9 (Danre)

Gene ID

5867 (RAB4A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of RAB4A / RAB4 antibody

Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound ictive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) . Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment . Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COOH-termil domains with a cysteine motif implicated in sub-cellular targeting. Post-translatiol modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins . Each Rab shows a characteristic sub-cellular distribution . In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin . Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-termil cdc2 kise consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol .

General readings

Li L. et al. (2001) J Biol Chem. 276:5265-73.
Lindsay AJ.. et al. (2002) J Biol Chem. 277:12190-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAB4A / RAB4 antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RAB4A mouse mAb with DAB staining. Mouse IgG1 4E11 Purified Hu, Mky, Ms E, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RAB4A / RAB4 antibody

Western blot analysis of RAB4A over-expressed 293 cell line, cotransfected with RAB4A Validated Chimera RNAi ( Cat # H00005867-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB4A monoclonal antibody (M01), clone 1C10 (Cat # H00005867-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG1 1C10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAB4A / RAB4 antibody

Western blot analysis of Rab4 in HeLa cell lysates using a 1:1000 dilution of the antibody Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAB4A / RAB4 antibody

WB Suggested Anti-RAB4A Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAB4A / RAB4 antibody

Western blot detection of RAB4A in Hela,PC-12,COS7 and mouse brain cell lysates using RAB4A mouse mAb (1:500 diluted).Predicted band size:24KDa.Observed band size:24KDa. Mouse IgG2a 3D1-A9-H10 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAB4A / RAB4 antibody

RAB4A MaxPab polyclonal antibody. Western Blot analysis of RAB4A expression in rat brain. Mouse Serum Hu, Rt E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RAB4A / RAB4 antibody

RAB4A MaxPab polyclonal antibody. Western Blot analysis of RAB4A expression in rat brain. Mouse Purified Hu, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RAB4A / RAB4 antibody

RAB4A / RAB4 Mouse IgG1 1E1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAB4A / RAB4 antibody

RAB4A / RAB4 Rabbit Serum Hu ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

RAB4A / RAB4 antibody

Immunohistochemistry-Paraffin: Rab4 Antibody [NBP1-46409] - Rat spinal cord (paraffin section) using Rabbit antibody to n-terminal region of Ras-related protein Rab-4A (RAB4A): whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. Rabbit Purified Hu, Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

RAB4A / RAB4 antibody

Immunofluorescence: Rab4 Antibody (EPR3043) [NBP1-95486] HeLa cells using anti-Rab4. Rabbit EPR3043 Purified Hu, Ms, Rt ICC/IF, IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAB4A / RAB4

RAB4A / RAB4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAB4A / RAB4 (1-218, His-tag)

Recombinant human RAB4A, 1-218aa, His-tagged Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

RAB4A / RAB4 (1-218, His-tag)

Recombinant human RAB4A, 1-218aa, His-tagged Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

RAB4A / RAB4

RAB4A / RAB4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RAB4A / RAB4

RAB4A / RAB4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RAB4A 293T Cell Transient Overexpression Lysate(Denatured)

RAB4A 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

17 Item(s)

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