RAB5A / RAB5 antibody

Principal name

RAB5A / RAB5 antibody

Alternative names for RAB5A / RAB5 antibody

Rab-5A, Ras-related protein Rab-5A

SwissProt ID

P18066 (Canfa), P20339 (Human), Q06AU6 (Pig), Q0IIG7 (Bovin), Q9CQD1 (Mouse)

Gene ID

5868 (RAB5A), 271457 (nnyRab5a)

Available reactivities

Bov (Bovine), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Can (Canine), GP (Guinea Pig), Hst (Hamster), Por (Porcine), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of RAB5A / RAB5 antibody

Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound ictive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) . Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment . Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable COOH-termil domains with a cysteine motif implicated in subcellular targeting. Post-translatiol modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. Each Rab shows a characteristic subcellular distribution . In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes . Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 . Anti-Rab5 may be used as an early endosome marker.

General readings

General / background references:Clague MJ Molecular aspects of the endocytic pathway. Biochem J 336 ( Pt 2):271-82 (1998). PubMed PMID: 9820800
Rybin V et al. GTPase activity of Rab5 acts as a timer for endocytic membrane fusion. Nature 383:266-9 (1996). PubMed PMID: 8805704
Chavrier P et al. Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments. Cell 62:317-29 (1990). PubMed PMID: 2115402
Zahraoui A et al. The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion. J Biol Chem 264:12394-401 (1989). PubMed PMID: 2501306

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

pan RAB5 (C-term) antibody

pan RAB5 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / €50.00
  SICGEN

pan RAB5 (C-term) antibody

pan RAB5 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.6 mg / €300.00
  SICGEN

RAB5A / RAB5 antibody

Predicted band size: 24kDa. Positive control: 231 cell, Human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 231 cell lysate Lane2: Human fetal brain tissue lysate Lysates: 30 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 antibody

Predicted band size: 24kDa. Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/1000-3000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/1300 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 (C-term) antibody

Western blot analysis of Rab5 Antibody (C-term) (Cat. #TA324884) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 antibody

Western blot analysis of Rab5 in a human cell line mix using a 1:1000 dilution of the antibody Rabbit Purified Bov, Hu, Mky, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 antibody

Western blot analysis of extracts of various cell lines, using RAB5A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 antibody

WB Suggested Anti-RAB5A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 antibody

WB Suggested Anti-RAB5A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 antibody

Western blot analysis of extract from Hela, MCF and PC12 cells using Rab5 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 antibody

Western blot analysis of Rab5A expression in HeLa cell lysate Rabbit IgG Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAB5A / RAB5 (C-term) antibody

RAB5A / RAB5 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.8 mg / €320.00
  SICGEN

RAB5A / RAB5 (C-term) antibody

RAB5A / RAB5 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.8 mg / €320.00
  SICGEN

RAB5A / RAB5 antibody

Western blot analysis on Mouse brain: The extracts of mouse brain (45 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 3A4 Purified Hu, Ms E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

RAB5A / RAB5 antibody

Western blot analysis on Mouse brain: The extracts of mouse brain (45 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 3A4 Purified Hu, Ms E, WB
50 µl / €230.00
  Acris Antibodies GmbH

RAB5A / RAB5 control peptide

RAB5A / RAB5 Purified
50 µg / €190.00
  Acris Antibodies GmbH

RAB5A / RAB5 antibody

Western blot analysis of Rab5 expression in heat shocked HeLa cell lysate. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt IP, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

RAB5A / RAB5 antibody

RAB5A monoclonal antibody (M09A), clone 5D1 Western Blot analysis of RAB5A expression in K-562 ( Cat # L009V1 ). Mouse IgM 5D1 Ascites Hu, Ms E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

RAB5A / RAB5 antibody

Western blot analysis of mouse brain extracts ( 45 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with RAB5A monoclonal antibody , clone 3A4 ( 1 : 500 ) ( Cat # MAB1109 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 3A4 Hu, Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

RAB5A / RAB5 antibody

Western blot analysis of RAB5A expression in heat shocked HeLa cell lysate. Rabbit Bov, Hu, Mky, Ms, Rt C, IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

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