Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of Rabenosyn-5 / ZFYVE20 antibody
Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3)
Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M. Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain. J Cell Biol. 2000 Oct 30;151(3):601-12. PMID: 11062261
NB 300-813 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Rabenosyn 5 Antibody [NBP1-33649] – Western blot analysis of Rabenosyn 5 on H1299 lysates (Lane A) and Hela cells (Lane B) using NBP1-33649 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel.
Human Stomach; WB Suggested Anti-ZFYVE20 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Stomach; ZFYVE20 antibody - N-terminal region (ARP39547_P050) in Human Stomach cells using Western Blot