RAC1 antibody

Principal name

RAC1 antibody

Alternative names for RAC1 antibody

p21-Rac1, Ras-like protein TC25, MIG5, Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein

SwissProt ID

P62998 (Bovin), P62999 (Canfa), P63000 (Human), P63001 (Mouse), P80236 (Cavpo), Q6RUV5 (Rat)

Gene ID

5879 (RAC1), 19353 (Rac1), 363875

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of RAC1 antibody

Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key sigling roles in cytoskeletal reorganization, membrane trafficking, transcriptiol regulation, cell growth and development. GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the proteins intrinsic GTPase activity, rendering it ictive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their ictive GDP-bound state.

General readings

Chrostek A., et al. (2006) Mol Cell Biol. 26: 6957-70.
Sun CX., et al. (2004) Blood. 104(12):3758-65.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAC1 antibody

  Rabbit Polyclonal Antibody against RAC1 (S71)  
Western blot analysis of RAC1 (arrow) using rabbit polyclonal RAC1 Antibody (S71) (Cat.#TA302136).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RAC1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAC1 antibody

  Rabbit Polyclonal antibody to RAC1 (ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1))  
Sample (50 ug of whole cell lysate). A: mouse liver. 12% SDS PAGE. TA308948 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAC1 antibody

  Mouse Anti-Human RAC1 Monoclonal Antibody  
Anti-RAC1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG2b Purified Hu, Ms, Rt
50 µg / €345.00
  OriGene Technologies, Inc.

RAC1 antibody

  Anti-RAC1 Rabbit Polyclonal Antibody  
Predicted band size: 23 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAC1 antibody

  Phospho-RAC1-S71 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from 293 cells, using phospho-RAC1-S71 antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAC1 antibody

  Rabbit anti-RAC1 Polyclonal Antibody  
Western blot analysis of extracts of 293 cell line, using RAC1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAC1 antibody

  Rabbit Polyclonal Anti-RAC1 Antibody - middle region  
WB Suggested Anti-RAC1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAC1 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using RAC1 antibody (AP02713PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

RAC1 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using RAC1 antibody (AP02713PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH

RAC1 antibody

Western blot analysis of RAC1 polyclonal antibody ( Cat # PAB2976 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the RAC1 gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

RAC1 antibody

Western blot analysis of the extracts from A-431 cells untreated or treated with EGF ( 200 ng/ml, 5 min ) using RAC1 polyclonal antibody ( Cat # PAB5543 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

RAC1 pSer71 antibody

Western blot analysis of extracts from 293 cells treated with PMA, using RAC1 (phospho S71) polyclonal antibody ( Cat # PAB7743 ). Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

RAC1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Rac1/CDC42 (Ab-71) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

RAC1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Rac1/CDC42 (Ab-71) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Rac1 - mouse gene knockout kit via CRISPR

Rac1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rac1 - mouse gene knockout kit via CRISPR

Rac1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rac1 - mouse gene knockout kit via CRISPR

Rac1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rac1 - mouse gene knockout kit via CRISPR

Rac1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAC1 (transcript variant Rac1)

  Recombinant protein of human ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) (RAC1), transcript variant Rac1  
RAC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAC1 (transcript variant Rac1b)

  Recombinant protein of human ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) (RAC1), transcript variant Rac1b  
RAC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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