RAD17 antibody

Principal name

RAD17 antibody

Alternative names for RAD17 antibody

R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog

SwissProt ID

O7594, O75943 (Human), O75943-2, P48581 (Yeast), P50531 (Schpo), Q6NXW6 (Mouse), Q9MBA3 (Arath)

Gene ID

5884 (RAD17), 854550, 19356 (Rad17), 2541643 (rad17)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), S. cerevisiae (Saccharomyces cerevisiae), Ye (Yeast), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (detection) (E(detection)), PLA/Proximity ligation assay (PLA)

Background of RAD17 antibody

RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.

General readings

1. Post, S., et al., Nucleic Acids Res. 31, 5568-5575 (2003)
2. Kataoka, A., et al., Clin. Cancer Res. 7, 2815-2820 (2001)
3. Post, S., et al., PNAS 98, 13102-13107 (2001)
4. Bao, S., et al., Cancer Res. 39, 2023-2082 (1999)
5. Chang, M. S., et al., J Biol. Chem. 274, 36544-36549 (1999)
Clone 31E9 is used in these references.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD17 antibody

RAD17 MaxPab rabbit polyclonal antibody. Western Blot analysis of RAD17 expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RAD17 antibody

Western blot analysis of extracts from HepG2 cells, using RAD17 (Ab-646) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD17 antibody

Western blot analysis of extracts of various cell lines, using RAD17 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD17 antibody

Western Blot: Rad17 Antibody [TA336266] Rabbit Purified Ye WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD17 antibody

Western Blot: Rad17 Antibody [TA336267] - WB detection of RAD17 protein in human heart lysate using TA336267 at a dilution of 1:500 Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD17 antibody

Western Blot: Rad17 [p Ser645] Antibody [TA336973] - analysis of phosphorylated Rad17 (Ser645) in 1) untreated PANC-1 cells (a human pancreatic carcinoma) and 2) UV-treated PANC-1 cells using this antibody at 0.5 ug/ml. Rabbit Purified Hu, Ms, Prim P, WB
20 µg / €325.00
  OriGene Technologies, Inc.

RAD17 antibody

Western blot analysis on HeLa cell lysate using RAD17 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD17 antibody

Immunohistochemistry: AM20179AF-N Rad17 antibody staining of Paraffin Embedded Human Lung Adenocarcinoma Section. Mouse IgG1 31E9 Azide Free Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

RAD17 pSer645 antibody

Detection of human phospho-Rad17 (pSer645) in whole cell lysates of HeLa cells either treated with 10Gy ionizing radiation or untreated by Western blotting using Rabbit anti Human phospho-Rad17 (pSer645) (AP05878PU-N) Rabbit IgG Purified Hu WB
0.1 mg / €550.00
  Acris Antibodies GmbH

RAD17 (Center) antibody

Western blot analysis of RAD17 Antibody Cat.-No AP53558PU-N in A549 cell line lysates (35ug/lane). This demonstrates the RAD17 antibody detected the RAD17 protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD17 antibody

Detection of human Rad 17 in whole cell lysates from HeLa cells treated with 10Gy ionizing radiation or untreated by Western blotting using Goat anti Human Rad17 (SP2111P) Goat Aff - Purified Hu WB
0.1 mg / €450.00
  Acris Antibodies GmbH

RAD17 antibody

Western Blot analysis of RAD17 expression in transfected 293T cell line (H00005884-T01) by RAD17 MaxPab polyclonal antibody.

Lane 1: RAD17 transfected lysate(64.24 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RAD17 antibody

Western Blot analysis of RAD17 expression in transfected 293T cell line (H00005884-T01) by RAD17 MaxPab polyclonal antibody.

Lane 1: RAD17 transfected lysate(64.24 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RAD17 antibody

RAD17 monoclonal antibody (M01), clone 2G12 Western Blot analysis of RAD17 expression in HeLa NE ( Cat # L013V3 ). Mouse IgG1 2G12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAD17 antibody

Western blot analysis of Rad17 in A ) a partial recombinant protein containing phospho Ser647 and B ) the same sequence without the phospho serine, and was blocked using the non-phospho control of the immunizing peptide. Using Rad17 polyclonal antibody ( Cat # PAB0223 ) at 0.1 µg/ml. Rabbit Ms ICC/IF, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

RAD17 pSer645 antibody

Western blot analysis of extracts from HeLa cells treated with UV 15', using RAD17 (Phospho-Ser645) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

RAD17 pSer645 antibody

Western blot analysis of extracts from HeLa cells treated with UV 15', using RAD17 (Phospho-Ser645) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

RAD17 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RAD17 (Ab-645) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

RAD17 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RAD17 (Ab-645) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

RAD17 antibody

Hela, HEK293T, Xenopus laevis egg extract, mouse embryonic; WB Suggested Anti-RAD17 Antibody . . Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate. Primary Antibody Dilution : 1:500. Secondary Antibody : Anti-rabbit-HRP . Secondry Antibody Dilution : 1:3000. Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS; RAD17 antibody - C-terminal region (ARP30206_T100) in Hela, HEK293T, Xenopus laevis egg extract, mouse embryonic cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

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