RAD18 / RNF73 antibody

Principal name

RAD18 / RNF73 antibody

Alternative names for RAD18 / RNF73 antibody

E3 ubiquitin-protein ligase RAD18, Postreplication repair protein RAD18, RING finger protein 73

SwissProt ID

O74747 (Schpo), P10862 (Yeast), Q9NS91 (Human), Q9QXK2 (Mouse)

Gene ID

56852 (RAD18)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunofluorescence (IF)

Background of RAD18 / RNF73 antibody

RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Tsuji,Y., (2008) Genes Cells 13 (4), 343-354

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD18 / RNF73 antibody

Immunoperoxidase of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml] Mouse IgG2b 3H7 Purified Hu, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAD18 / RNF73 antibody

Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308755 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

Western blot analysis of extracts from LOVO cells, HepG2 cells and K562 cells, using RAD18 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

WB Suggested Anti-RAD18 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

WB Suggested Anti-RAD18 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

Western blot analysis of extracts of various cell lines, using RAD18 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

Western Blot: RAD18 Antibody (79B1048.1) [TA336384] - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate. Mouse IgG1 79B1048.1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

Western blot analysis on LOVO cell lysate using RAD18 Antibody Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

RAD18 / RNF73 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RAD18 / RNF73 antibody

RAD18 / RNF73 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RAD18 / RNF73 antibody

RAD18 / RNF73 Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

RAD18 / RNF73 antibody

RAD18 MaxPab rabbit polyclonal antibody. Western Blot analysis of RAD18 expression in mouse stomach. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RAD18 / RNF73 antibody

RAD18 monoclonal antibody (M03), clone 2B9. Western Blot analysis of RAD18 expression in Hela NE ( Cat # L013V3 ). Mouse IgG2b 2B9 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAD18 / RNF73 antibody

Human 721_B; WB Suggested Anti-RAD18 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate; RAD18 antibody - middle region (ARP43266_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

RAD18 / RNF73 antibody

RAD18 / RNF73 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RAD18 / RNF73 antibody

Western blot analysis of RAD18 expression in MCF7 (A); A549 (B); Jurkat (C); HCT116 (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Por IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RAD18 / RNF73 antibody

Western blot analysis of RAD18 expression in MCF7 (A); A549 (B); Jurkat (C); HCT116 (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Por IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RAD18 / RNF73 antibody

Western blot analysis of RAD18 expression in MCF7 (A); A549 (B); Jurkat (C); HCT116 (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Por IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

RAD18 / RNF73 antibody

RAD18 / RNF73 Rabbit Purified Hu IF, IP, WB
0.1 mg / €510.00
  Funakoshi Co., Ltd.

RAD18 / RNF73 antibody

Western Blot: RAD18 Antibody [NB100-61062] - Detection of Human RAD18 by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAD18 antibody NB100-61062 used for IP at 3 mcg/mg lysate. RAD18 was also immunoprecipitated by rabbit anti- RAD18 antibody NB100-61063, which recognizes a downstream epitope.  Detection: Chemiluminescence with an exposure time of 3 seconds. Rabbit IgG Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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