RAD18 / RNF73 antibody

Principal name

RAD18 / RNF73 antibody

Alternative names for RAD18 / RNF73 antibody

E3 ubiquitin-protein ligase RAD18, Postreplication repair protein RAD18, RING finger protein 73

SwissProt ID

O74747 (Schpo), P10862 (Yeast), Q9NS91 (Human), Q9QXK2 (Mouse)

Gene ID

56852 (RAD18), 58186 (Rad18)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunofluorescence (IF)

Background of RAD18 / RNF73 antibody

RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Tsuji,Y., (2008) Genes Cells 13 (4), 343-354

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD18 / RNF73 antibody

  Rabbit polyclonal antibody to RAD18 (RAD18 homolog (S. cerevisiae))  
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308755 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

  Rabbit polyclonal anti-RAD18 antibody  
Western blot analysis of extracts from LOVO cells, HepG2 cells and K562 cells, using RAD18 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

  Rabbit Polyclonal Anti-RAD18 Antibody  
WB Suggested Anti-RAD18 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

  Rabbit Polyclonal Anti-RAD18 Antibody  
WB Suggested Anti-RAD18 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

  Rabbit anti-RAD18 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using RAD18 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

  Mouse Monoclonal RAD18 Antibody (79B1048.1)  
Western Blot: RAD18 Antibody (79B1048.1) [TA336384] - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate. Mouse IgG1 79B1048.1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD18 / RNF73 antibody

  Rabbit Polyclonal Anti-RAD18 Antibody  
Western blot analysis on LOVO cell lysate using RAD18 Antibody Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

RAD18 - human gene knockout kit via CRISPR

RAD18 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RAD18 - human gene knockout kit via CRISPR

RAD18 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RAD18 - human gene knockout kit via CRISPR

RAD18 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RAD18 - human gene knockout kit via CRISPR

RAD18 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAD18 / RNF73

  Recombinant protein of human RAD18 homolog (S. cerevisiae) (RAD18)  
RAD18 / RNF73 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

RAD18 overexpression lysate

RAD18 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RAD18 (mGFP-tagged) - Human RAD18 homolog (S. cerevisiae) (RAD18), 200 uL, >10^7 TU/mL

Lenti ORF particles, RAD18 (mGFP-tagged) - Human RAD18 homolog (S. cerevisiae) (RAD18), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, RAD18 (Myc-DDK tagged) - Human RAD18 homolog (S. cerevisiae) (RAD18) , 200 uL, >10^7 TU/mL

Lenti ORF particles, RAD18 (Myc-DDK tagged) - Human RAD18 homolog (S. cerevisiae) (RAD18) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rad18 (GFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rad18 (GFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,190.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rad18 (GFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rad18 (GFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,130.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,070.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,010.00
  OriGene Technologies, Inc.

19 Item(s)

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