RAD23B antibody

Principal name

RAD23B antibody

Alternative names for RAD23B antibody

HR23B, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein

SwissProt ID

P54727 (Human), P54728 (Mouse), Q29RK4 (Bovin), Q4KMA2 (Rat), Q84L32 (Arath)

Gene ID

5887 (RAD23B), 4588745 (A1DB76)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Ye (Yeast), Can (Canine), S. cerevisiae (Saccharomyces cerevisiae), Bov (Bovine), Ze (Zebrafish), African clawed frog, Mky (Monkey), Hst (Hamster)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of RAD23B antibody

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

General readings

Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224 (2010).
Guillem, V.M., et al. Am. J. Hematol. 85(7):482-486 (2010).
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263 (2010).
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press.
Rajaraman, P., et al. Neuro-oncology 12(1):37-48 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD23B antibody

Western blot detection of hHR23b in A431,K562,Jurkat,C6,3T3 and Hela cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min. Mouse IgG2b 5H1-A10-A7 Purified Hst ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD23B antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RAD23B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804867) Mouse IgG1 OTI7G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

Immunoperoxidase of purified MaxPab antibody to RAD23B on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RAD23B antibody

Western blot analysis of extracts from A549 cells and HUVEC cells, using RAD23B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD23B (163-176) antibody

WB using Anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate.  Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown).   Primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at RT. Goat IgG Aff - Purified Can, Chimp, Hu, S. cerevisiae E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD23B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG1 OTI7F11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RAD23B Mouse monoclonal antibody. (TA804863; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI9F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG1 OTI13A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG1 OTI7C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG2b OTI12C10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG2a OTI13A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG1 OTI8E5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B (163-176) antibody

Figure 1. AP07626PU-N RAD23B antibody staining of Formalin-Fixed Paraffin-Embedded Human Prostate. Goat Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RAD23B control peptide

RAD23B Purified
50 µg / €190.00
  Acris Antibodies GmbH

RAD23B (N-term) antibody

Western blot analysis of RAD23B Antibody (N-term) Cat.-No AP53559PU-N in A549 cell line lysates (35ug/lane). This demonstrates the RAD23B antibody detected the RAD23B protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD23B antibody

Immunofluorescence of purified MaxPab antibody to RAD23B on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu, Rt E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RAD23B antibody

RAD23B MaxPab rabbit polyclonal antibody. Western Blot analysis of RAD23B expression in mouse kidney. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RAD23B antibody

RAD23B Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RAD23B antibody

Immunoperoxidase of monoclonal antibody to RAD23B on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml] Mouse IgG2b 3H7 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAD23B antibody

Western blot using RAD23B polyclonal antibody ( Cat # PAB10058 ) shows detection of a band at ~58 kDa ( arrowhead ) corresponding to RAD23B present in a HeLa whole cell lysate.
Pre-incubation of antibody with immunizing peptide completely blocks reactivity ( data notshown ).
Approximately 33 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS.
The membrane was washed and reacted with a 1:20,000 dilution of IRDyeTM800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature ( 800 nmchannel, green ).
Molecular weight estimation was made by comparison to prestained MW markers indicated at left ( 700 nm channel, red ).
IRDyeTM800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR,Inc. Chimp, Hu, Ms, Rt, Ye
0.1 mg / €560.00
  Abnova Taiwan Corp.

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