RAD23B antibody

Principal name

RAD23B antibody

Alternative names for RAD23B antibody

HR23B, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein

SwissProt ID

P54727 (Human), P54728 (Mouse), Q29RK4 (Bovin), Q4KMA2 (Rat), Q84L32 (Arath)

Gene ID

5887 (RAD23B)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Ye (Yeast), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Mky (Monkey), S. cerevisiae (Saccharomyces cerevisiae), Hst (Hamster)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of RAD23B antibody

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-D glycosylase (MPG), which suggested a role in D damage recognition in base excision repair. This protein contains an N-termil ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Altertive splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011].

General readings

Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224 (2010).
Guillem, V.M., et al. Am. J. Hematol. 85(7):482-486 (2010).
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263 (2010).
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press.
Rajaraman, P., et al. Neuro-oncology 12(1):37-48 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD23B antibody

  Mouse Monoclonal hHR23b Antibody  
Western blot detection of hHR23b in A431,K562,Jurkat,C6,3T3 and Hela cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min. Mouse IgG2b 5H1-A10-A7 Purified Hst ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD23B antibody

  RAD23B mouse monoclonal antibody, clone OTI7G10 (formerly 7G10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RAD23B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804867) Mouse IgG1 OTI7G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

  Rabbit polyclonal anti-RAD23B antibody  
Western blot analysis of extracts from A549 cells and HUVEC cells, using RAD23B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD23B (163-176) antibody

  Goat polyclonal anti-HR23B antibody  
WB using Anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate.  Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown).   Primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at RT. Goat IgG Aff - Purified Can, Chimp, Hu, S. cerevisiae E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

RAD23B antibody

  Mouse Monoclonal anti-Rad23B Antibody  
Jurkat whole cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-Rad23B antibody (clone 11A2SC). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse 11A2SC Purified Hu WB
25 µg / €280.00
  OriGene Technologies, Inc.

RAD23B antibody

  RAD23B mouse monoclonal antibody, clone OTI7F11 (formerly 7F11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG1 OTI7F11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

  RAD23B mouse monoclonal antibody, clone OTI9F8 (formerly 9F8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RAD23B Mouse monoclonal antibody. (TA804863; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI9F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

  RAD23B mouse monoclonal antibody, clone OTI13A3 (formerly 13A3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG1 OTI13A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

  RAD23B mouse monoclonal antibody, clone OTI7C5 (formerly 7C5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG1 OTI7C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

  RAD23B mouse monoclonal antibody, clone OTI12C10 (formerly 12C10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG2b OTI12C10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

  RAD23B mouse monoclonal antibody, clone OTI13A2 (formerly 13A2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG2a OTI13A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B antibody

  RAD23B mouse monoclonal antibody, clone OTI8E5 (formerly 8E5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD23B (RC202185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD23B. Mouse IgG1 OTI8E5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD23B (163-176) antibody

Figure 1. AP07626PU-N RAD23B antibody staining of Formalin-Fixed Paraffin-Embedded Human Prostate. Goat Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RAD23B (N-term) antibody

Western blot analysis of RAD23B Antibody (N-term) Cat.-No AP53559PU-N in A549 cell line lysates (35ug/lane). This demonstrates the RAD23B antibody detected the RAD23B protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD23B antibody

Western blot analysis of extracts from A549/HuvEc cells, using RAD23B Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

RAD23B antibody

Western blot analysis of extracts from A549/HuvEc cells, using RAD23B Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

RAD23B - human gene knockout kit via CRISPR

RAD23B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RAD23B - human gene knockout kit via CRISPR

RAD23B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RAD23B - human gene knockout kit via CRISPR

RAD23B - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RAD23B - human gene knockout kit via CRISPR

RAD23B - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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