RAD50 antibody

Principal name

RAD50 antibody

Alternative names for RAD50 antibody

DNA repair protein RAD50

SwissProt ID

O44199 (Caeel), P12753 (Yeast), P70388 (Mouse), Q92878 (Human), Q960E0 (Drome), Q9JIL8 (Rat), Q9SL02 (Arath), Q9UTJ8 (Schpo), Q9W252 (Drome)

Gene ID

10111 (RAD50), 855471

Available reactivities

Hu (Human), Hst (Hamster), Ms (Mouse), Rt (Rat), Ye (Yeast), Chk (Chicken), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E)

Background of RAD50 antibody

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. The hMre11-hRad50-NBS1 protein plays a central role in the human cellular DNA-Damage response, with recent studies indicating that these proteins help link DNA-damage detection to DNA-repair and cell cycle-checkpoint functions. This protein complex has been implicated in the activation of cell cycle-regulatory pathways, due to the fact that the NBS1 gene mutates in the chromosomal-instability syndrome, Nijmegen breakdown syndrome (NBS), which is characterized by increased cancer incidence, cell cycle checkpoint defects, and ionizing radiation sensitivity. HMre11-hRad50 foci form in response to DNA double-strand breaks and rely upon a DNA damage-induced signaling pathway. However, hMre11 migrates to sites of damage while hRad51 does not localize at these sites. These findings are consistent with the distinct role of these proteins in DNA repair.

General readings

1. Carney, J.P., et al. The hMre11/hRad50 Protein Complex and Nijmegen Breakage Syndrome: Linkage of Double-Strand Break Repair to the Cellular DNA Damage Response. Cell. 93: 477-486, 1998. (Western Blot, Immunoprecipitation, Immunofluorescence, Human)
2. Lim, et al. ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway. Nature. 404: 613-617, 2000.
See individual datasheets.
1. Takemura, Haruyuki., et al. DNA: replication, repair, and recombination: Defective MRE11-dependent activation of CHk2 by Ataxia Telangiectasia Mutated in Colorectal Carcinoma Cells in Response to Replication-dependent DNA Double Strand Breaks. 281: 30814-30823, 2006.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD50 antibody

Detection of Rad50. Lane 1: 25 ng purified hMre11-hRad50 complex. Lane 2: 25 ug crude HeLa extract Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Western blot analysis of extracts from COLO205 cells, 293 cells and HUVEC cells, using RAD50 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Predicted band size: 154 kDa . Positive control: 293T, Hela, K562, NIH/3T3 and RAW264.7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lane3: K562 cell lysate Lane4: NIH/3T3 cell lysate Lane5: RAW264.7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Predicted band size: 154 kDa . Positive control: 293T and Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Host: Rabbit; Target Name: RAD50; Sample Tissue: PANC1 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Western Blot: RAD50 Antibody [TA336262] - Detection of Rad50 in HeLa nuclear extracts. Rabbit Purified Hst, Hu, Ms IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

ELISA: RAD50 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Detection of human Rad50 immunoprecipitated from nuclear extracts of HeLa cells by Western blotting using Rabbit anti Human Rad50 (AP05756PU-N) Rabbit IgG Purified Hu, Ms, Rt IP, WB
50 µg / €320.00
  Acris Antibodies GmbH

RAD50 antibody

Western blot analysis of RAD50 in 25 ng purified hMre11-hRAD50 complex ( Lane 1 ) and 25 µg crude HeLa extract ( Lane 2 : ) with RAD50 polyclonal antibody ( Cat# PAB12052 ). Rabbit Hu IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

RAD50 antibody

RAD50 Rabbit Hst, Hu, Ms IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

RAD50 antibody

Western blot analysis of extracts from COLO205/293/HuvEc cells, using RAD50 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

RAD50 antibody

Western blot analysis of extracts from COLO205/293/HuvEc cells, using RAD50 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

RAD50 antibody

Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RAD50 antibody

Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RAD50 antibody

Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

RAD50 antibody

ELISA: RAD50 Antibody [22100002] Rabbit Hu E, WB
50 µg / €440.00
  Novus Biologicals Inc.

RAD50 antibody

RAD50 Rabbit Serum Hst, Hu, Ms IP, WB
25 µl / €200.00
  Novus Biologicals Inc.

RAD50 antibody

Immunohistochemistry:  Mre11-Rad50-NBS1 Complex Antibody [NB100-914] - MRE11 staining in the human epidermis detected. Rabbit var. Hu, Ms P, WB
3 vials / €620.00
  Novus Biologicals Inc.

RAD50 antibody

RAD50 Rabbit Hu IP, WB
25 µl / €170.00
  Novus Biologicals Inc.

RAD50 antibody

Immunohistochemistry-Paraffin: RAD50 Antibody [NB100-1488] - See Notes, IHC Caption B Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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