RAD50 antibody

Principal name

RAD50 antibody

Alternative names for RAD50 antibody

DNA repair protein RAD50

SwissProt ID

O44199 (Caeel), P12753 (Yeast), P70388 (Mouse), Q92878 (Human), Q960E0 (Drome), Q9JIL8 (Rat), Q9SL02 (Arath), Q9UTJ8 (Schpo), Q9W252 (Drome)

Gene ID

10111 (RAD50)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Chk (Chicken), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of RAD50 antibody

RAD50 (D repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and sigling of D double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint sigling, and as repair-effector in two competing DSB repair pathways: homologous recombition (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintence. RAD50 is required to bind D ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining D templates, and also stimulate the activity of D ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombition etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).

15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD50 antibody

Detection of Rad50. Lane 1: 25 ng purified hMre11-hRad50 complex. Lane 2: 25 ug crude HeLa extract Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Western blot analysis of extracts from COLO205 cells, 293 cells and HUVEC cells, using RAD50 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Predicted band size: 154 kDa . Positive control: 293T, Hela, K562, NIH/3T3 and RAW264.7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lane3: K562 cell lysate Lane4: NIH/3T3 cell lysate Lane5: RAW264.7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Predicted band size: 154 kDa . Positive control: 293T and Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Host: Rabbit; Target Name: RAD50; Sample Tissue: PANC1 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Western Blot: RAD50 Antibody [TA336262] - Detection of Rad50 in HeLa nuclear extracts. Rabbit Purified Hst, Hu, Ms IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

ELISA: RAD50 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Detection of human Rad50 immunoprecipitated from nuclear extracts of HeLa cells by Western blotting using Rabbit anti Human Rad50 (AP05756PU-N) Rabbit IgG Purified Hu, Ms, Rt IP, WB
50 µg / €320.00
  Acris Antibodies GmbH

RAD50 antibody

Western blot analysis of RAD50 in 25 ng purified hMre11-hRAD50 complex ( Lane 1 ) and 25 µg crude HeLa extract ( Lane 2 : ) with RAD50 polyclonal antibody ( Cat# PAB12052 ). Rabbit Hu IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

RAD50 antibody

RAD50 Rabbit Hst, Hu, Ms IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

RAD50 antibody

Western blot analysis of extracts from COLO205/293/HuvEc cells, using RAD50 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

RAD50 antibody

Western blot analysis of extracts from COLO205/293/HuvEc cells, using RAD50 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

RAD50 antibody

Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RAD50 antibody

Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RAD50 antibody

Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

15 Item(s)

per page
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