RAD50 antibody

Principal name

RAD50 antibody

Alternative names for RAD50 antibody

DNA repair protein RAD50

SwissProt ID

O44199 (Caeel), P12753 (Yeast), P70388 (Mouse), Q92878 (Human), Q960E0 (Drome), Q9JIL8 (Rat), Q9SL02 (Arath), Q9UTJ8 (Schpo), Q9W252 (Drome)

Gene ID

10111 (RAD50)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Chk (Chicken), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of RAD50 antibody

RAD50 (D repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and sigling of D double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint sigling, and as repair-effector in two competing DSB repair pathways: homologous recombition (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintence. RAD50 is required to bind D ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining D templates, and also stimulate the activity of D ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombition etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD50 antibody

  Rabbit Polyclonal Antibody against RAD50 (zinc hook domain)  
Detection of Rad50. Lane 1: 25 ng purified hMre11-hRad50 complex. Lane 2: 25 ug crude HeLa extract Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

  Rabbit polyclonal anti-RAD50 antibody  
Western blot analysis of extracts from COLO205 cells, 293 cells and HUVEC cells, using RAD50 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

  Anti-RAD50 Rabbit Polyclonal Antibody  
Predicted band size: 154 kDa . Positive control: 293T, Hela, K562, NIH/3T3 and RAW264.7 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lane3: K562 cell lysate Lane4: NIH/3T3 cell lysate Lane5: RAW264.7 cell lysate Lysates: 40 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

  Anti-RAD50 Rabbit Polyclonal Antibody  
Predicted band size: 154 kDa . Positive control: 293T and Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

  Rabbit Polyclonal Anti-RAD50 Antibody  
Host: Rabbit; Target Name: RAD50; Sample Tissue: PANC1 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

  Rabbit Polyclonal Rad50 Antibody  
Western Blot: RAD50 Antibody [TA336262] - Detection of Rad50 in HeLa nuclear extracts. Rabbit Purified Hst, Hu, Ms IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

  Rabbit Polyclonal RAD50 Antibody  
ELISA: RAD50 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD50 antibody

Detection of human Rad50 immunoprecipitated from nuclear extracts of HeLa cells by Western blotting using Rabbit anti Human Rad50 (AP05756PU-N) Rabbit IgG Purified Hu, Ms, Rt IP, WB
50 µg / €320.00
  Acris Antibodies GmbH

RAD50 antibody

Western blot analysis of extracts from COLO205/293/HuvEc cells, using RAD50 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

RAD50 antibody

Western blot analysis of extracts from COLO205/293/HuvEc cells, using RAD50 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

RAD50 - human gene knockout kit via CRISPR

RAD50 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RAD50 - human gene knockout kit via CRISPR

RAD50 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RAD50 - human gene knockout kit via CRISPR

RAD50 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RAD50 - human gene knockout kit via CRISPR

RAD50 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RAD50 (mGFP-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50), 200 uL, >10^7 TU/mL

Lenti ORF particles, RAD50 (mGFP-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50), 200 uL, >10^7 TU/mL

Lenti ORF particles, RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50), 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

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