RAD52 antibody

Principal name

RAD52 antibody

Alternative names for RAD52 antibody

DNA repair protein RAD52 homolog

SwissProt ID

P06778 (Yeast), P39022 (Chick), P43351 (Human), P43352 (Mouse)

Gene ID

5893 (RAD52), 175893 (gar-2)

Available reactivities

Hu (Human), Ms (Mouse), Mam (Mammalia), Bov (Bovine), Mky (Monkey), Por (Porcine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunofluorescence (IF), Immunoprecipitation (IP)

Background of RAD52 antibody

DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved. Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule. RAD54B is significantly homologous to the RAD54 recombination gene. Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination.

General readings

Liu, Y., et al. Carcinogenesis 31(10):1762-1769 (2010).
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press.
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224 (2010).
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010).
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD52 antibody

WB analysis using Anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B).   ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Peptide competition (panel A) blocks the specific staining of this band.  The blot was incubated with a 1:1000 dilution of the antibody followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L]  diluted 1:5,000. Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD52 antibody

Predicted band size: 46 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 30 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD52 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using RAD52 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD52 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG 5H9 Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RAD52 antibody

RAD52 Rabbit IgG Purified Hu E, WB
50 µg / €320.00
  Acris Antibodies GmbH

RAD52 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Prostate. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RAD52 antibody

Immunohistochemistry analyzes of Rad52 antibody (AP20268PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RAD52 (Center) antibody

Western blot analysis of RAD52 Antibody (Center) Cat.-No AP53563PU-N in CEM cell line lysates (35ug/lane). This demonstrates the RAD52 antibody detected the RAD52 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD52 (C-term) antibody

Western blot analysis of RAD52 Antibody (C-term) Cat.-No AP53564PU-N in NCI-H292 cell line lysates (35ug/lane). This demonstrates the RAD52 antibody detected the RAD52 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD52 antibody

RAD52 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

RAD52 antibody

Western blot analysis is shown using RAD52 polyclonal antibody ( Cat # PAB10230 ) to detect human RAD52 present in a HeLa nuclear extract ( panel B ).
Approximately 30 µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.
Comparison to a molecular weight marker ( not shown ) indicates a band of ~ 63.0 kDa is detected.
Peptide competition ( panel A ) blocks the specific staining of this band.
The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDyeTM800 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 5,000 for 45 min. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

RAD52 pTyr104 antibody

Immunohistochemistry analysis of paraffin-embedded human testis, using RAD52 (Phospho-Tyr104) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

RAD52 pTyr104 antibody

Immunohistochemistry analysis of paraffin-embedded human testis, using RAD52 (Phospho-Tyr104) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

RAD52 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using RAD52 (Ab-104) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

RAD52 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using RAD52 (Ab-104) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

RAD52 pTyr104 antibody

Western blot analysis of RAD52 (pY104) expression in HepG2 (A); mouse spleen (B); rat spleen (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Por, Rt WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

RAD52 pTyr104 antibody

Western blot analysis of RAD52 (pY104) expression in HepG2 (A); mouse spleen (B); rat spleen (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Por, Rt WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

RAD52 pTyr104 antibody

Western blot analysis of RAD52 (pY104) expression in HepG2 (A); mouse spleen (B); rat spleen (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Por, Rt WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

RAD52 antibody

RAD52 Rabbit Aff - Purified Hu, Mam IF, IP, WB
50 µg / €410.00
  Funakoshi Co., Ltd.

RAD52 antibody

RAD52 Mouse 5H9 Purified Hu P, WB
50 µg / €480.00
  Novus Biologicals Inc.

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