RAD54L antibody

Principal name

RAD54L antibody

Alternative names for RAD54L antibody

RAD54A, RAD54 homolog, hRAD54, hHR54, DNA repair and recombination protein RAD54-like

SwissProt ID

O12944 (Chick), P32863 (Yeast), P41410 (Schpo), P70270 (Mouse), Q92698 (Human)

Gene ID

8438 (RAD54L)

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F)

Background of RAD54L antibody

This protein belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination.The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Liu, Y., et al. Carcinogenesis 31(10):1762-1769 (2010).
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224 (2010).
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press.
Guey, L.T., et al. Eur. Urol. 57(2):283-292 (2010).
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853 (2009).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD54L antibody

Western blot analysis of extracts from HeLa cells, Jurkat cells and 293 cells, using RAD54 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD54L antibody

WB Suggested Anti-RAD54L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD54L (1-17) antibody

Figure 1. Model for generation of supercoiling by hRad54 translocation along DNA. The hRad54 complex and plasmid DNA are indicated by the shaded oval and black line, respectively. (A) Movement of the hRad54 complex by tracking along the helical path of DNA is indicated by the arrows. When the complex is free to rotate around the DNA, no change in supercoiling will be induced in the plasmid DNA. (B) When the hRad54 complex tracks along the helix, while being prevented from rotating around the DNA, positive supercoils will arise ahead of the protein complex and negative supercoils behind it. These supercoils can freely distribute along the plasmid and therefore they will cancel each other out. (C) The interaction of two hRad54 complexes on a plasmid will divide the plasmid into two domains. Because the plasmid is singly nicked, one domain will contain a nick, whereas the other contains two covalently closed DNA strands. Depending on the position of the nick relative to the movement of the  protein complex along the DNA, topoisomers containing either negative or positive supercoils will result after ligation of the nick. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

RAD54L (C-term) antibody

Flow cytometric analysis of NCI-H460 cells using RAD54L Antibody (C-term) Cat.-No AP53565PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD54L antibody

RAD54L Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RAD54L antibody

RAD54L Mouse IgG3 4G2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAD54L antibody

Human Placenta; WB Suggested Anti-RAD54L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; RAD54L antibody - N-terminal region (ARP36355_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

RAD54L antibody

Western blot:RAD54L Antibody [NBP1-31892]- (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE Rabbit IgG Hu WB
0.1 ml / €420.00
  Novus Biologicals Inc.

RAD54L antibody

RAD54L Mouse 38.4 Hu E, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

RAD54L antibody

Western Blot: RAD54L Antibody [NBP1-58228] - This Anti-RAD54L antibody was used in Western Blot of Placenta tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAD54L (transcript variant 1)

RAD54L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAD54L (transcript variant 2)

RAD54L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAD54L

RAD54L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RAD54L

RAD54L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RAD54L overexpression lysate

RAD54L overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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