RAD9A antibody

Principal name

RAD9A antibody

Alternative names for RAD9A antibody

RAD-9A, Cell cycle checkpoint control protein RAD9A, EC=3.1.11.2, DNA repair exonuclease rad9 homolog A

SwissProt ID

Q4R5X9 (Macfa), Q99638 (Human), Q9Z0F6 (Mouse)

Gene ID

5883 (RAD9A), 19367 (Rad9)

Available reactivities

Hu (Human), Ye (Yeast), Ms (Mouse), Can (Canine), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of RAD9A antibody

Cells respond to DNA damage by activating a network of signaling pathways that control cell cycle progression and DNA repair. Cell cycle checkpoints are mechanisms that transiently delay cell cycle progression when DNA is damaged or DNA replication is incomplete. In the fission yeast Schizosaccharomyces pombe, a group of six such checkpoint control genes have been identified and include rad1+, rad3+, rad9+, rad17+, rad26+ and hus1+. Mutations in any one of these genes render cells sensitive to gamma-rays, UV light or the DNA synthesis inhibitor hydroxyurea (HU) and eliminate the ability of cells to delay entry into mitosis after treatment with these agents. All of these genes apparently link abnormal DNA structures to cell cycle control. As for cell cycle-related genes in general, these checkpoint control genes are highly conserved throughout evolution. Human and mouse versions of several of the S.pombe genes have been isolated, providing strong evidence that checkpoint control mechanisms are also highly conserved. In mammals, these genes are thought to maintain genomic stability, especially in the presence of DNA damage. Therefore, when these genes are altered, genomic instability may occur and lead to cancer. The biochemical activities of most of the checkpoint control gene products are not well established, although progress has been made towards learning more about their function. For example, examination of the structure of the protein encoded by human or S.pombe rad9 reveals a BH3-like domain in the N-terminal region that can bind the anti-apoptotic proteins Bcl-2 and Bcl-XL. Furthermore, overexpression of the gene from either organism in human cells can cause apoptosis in a BH3 domain-dependent manner. Both S.pombe and human versions of the protein can bind two other checkpoint control proteins, Hus1p (HUS1p) and Rad1p (RAD1p). Human RAD9 protein binds HUS1 and RAD1 proteins at its C-terminal region, suggesting that RAD9 has at least two functional domains, one involved in apoptosis and the other in cell cycle checkpoint control. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphosphorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate.

General readings

This product has been used in:Komatsu K et al. PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition. Oncogene 19:5291-7 (2000). PubMed PMID: 11077446
General / background references:Brondello JM et al. Basis for the checkpoint signal specificity that regulates Chk1 and Cds1 protein kinases. Mol Cell Biol 19:4262-9 (1999). PubMed PMID: 10330167
Russell P Checkpoints on the road to mitosis. Trends Biochem Sci 23:399-402 (1998). PubMed PMID: 9810229
Lieberman HB et al. A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene. Proc Natl Acad Sci U S A 93:13890-5 (1996). PubMed PMID: 8943031

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAD9A antibody

Sample (30 ug of whole cell lysate). A: A431 . B: Hela. 10% SDS PAGE. TA308749 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD9A antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500434, Dilution 1:50) Mouse IgG2a OTI7B11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RAD9A antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500435, Dilution 1:50) Mouse IgG2a OTI5D9 Purified Can, Hu, Mky P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RAD9A antibody

Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9Amouse monoclonal antibody. (Dilution 1:50) Mouse 7B11 Aff - Purified Hu P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

RAD9A antibody

Western Blot: Rad9 Antibody - Analysis of Rad9 on HeLa whole cell extracts using Rad9 antibody. Rabbit IgG Purified Hu C, WB
50 µl / €325.00
  OriGene Technologies, Inc.

RAD9A (BH3 Domain) antibody

Western blot analysis of anti-Rad9 BH3 domain  Pab (Cat. #TA302093) in HL-60 cell lysate. Rad9 BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD9A (C-term) antibody

TA302588 (0.3µg/ml) staining of A431cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD9A antibody

Predicted band size: 42 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD9A antibody

WB Suggested Anti-RAD9A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysateRAD9A is supported by BioGPS gene expression data to be expressed in MCF7 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAD9A antibody

Western Blot: Rad9 Antibody (93A535) [TA336578] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test. Mouse IgG1 93A535 Purified Hu C, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAD9A antibody

Western blot detection of RAD9A in Hela,MCF7,3T3,COS7 and Rat testis cell lysates using RAD9A mouse mAb (1:500 diluted).Predicted band size:43KDa.Observed band size:55KDa. Mouse IgG2b 3A3-A7-F8 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAD9A antibody

RAD9A Rabbit IgG Purified Ye E, WB
50 µg / €310.00
  Acris Antibodies GmbH

RAD9A pSer272 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD9A pSer277 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD9A pSer328 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD9A pSer387 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD9A pTyr28 antibody

The anti-Phospho-Rad9 pTyr28 Pab (Cat.#AP12692PU-N) is used in Western blot to detect Phospho-Rad9 pTyr28 in HL30 tissue lysate Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD9A (Center) antibody

Western blot analysis of anti-Rad9 Antibody (Center) in Hela cell line lysates (35ug/lane). Rad9 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD9A antibody

Formalin fixed, paraffin embedded human breast carcinoma stained with RAD9A Antibody Cat.-No AP53568PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAD9A (pan reactive) antibody

RAD9A Rabbit Aff - Purified Ye E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

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