RAF1 antibody

Principal name

RAF1 antibody

Alternative names for RAF1 antibody

C-RAF, Raf-1, cRaf, RAF proto-oncogene serine/threonine-protein kinase

SwissProt ID

A7E3S4 (Bovin), P04049 (Human), P05625 (Chick), P09560 (Xenla), P11345 (Rat), Q99N57 (Mouse)

Gene ID

5894 (RAF1), 24703 (Raf1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Xen (Xenopus), Por (Porcine)

Available hosts

Rabbit, Mouse, Rat, Chicken

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), ELISA (detection) (E(detection)), Frozen Sections (C), Flow Cytometry (F)

Background of RAF1 antibody

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAF1 antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAF1 antibody Cat.-No AM06729PU-N with DAB staining. Mouse IgG1 4G4 Purified Hu E, F, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RAF1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PDGFRB and RAF1. Huh7 cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-RAF1 mouse monoclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2b 1H4 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAF1 antibody

Western blot analysis of extracts from HT-29 cells, using Raf1 (Ab-341) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

Western blot analysis of extracts from HeLa cells, treated with UV (5mins), using Raf1 (Ab-621) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

Western blot analysis of extracts from CoLo cells, using C-RAF (Ab-642) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

Anti-RAF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG1 Purified Hu E
50 µg / €345.00
  OriGene Technologies, Inc.

RAF1 (N-term) antibody

Western blot analysis of C-raf in 3T3 cell lysate with C-raf antibody at (A) 0.5 and (B) 1 ug/mL. Chicken Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

Western blot analysis of C-RAF expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

Western blot analysis of C-RAF expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

Western blot analysis on Jurkat cell lysate using C-RAF Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAF1 pSer621 antibody

RAF1 Mouse IgG2a 6B4 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RAF1 antibody

Immunoblot Analysis A431 cell lysate probed with mab C-Raf (PBB-1) at 0.1 µg/ml. Mouse IgG1 PBB-1 Purified Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

RAF1 (N-term) antibody

RAF1 Mouse IgG1 540 Purified Hu E, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

RAF1 (N-term) antibody

RAF1 Mouse IgG1 863 Purified Hu E, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

RAF1 (N-term) antibody

RAF1 Mouse IgG2b 163 Purified Hu E, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

RAF1 control peptide

RAF1
50 µg / €160.00
  Acris Antibodies GmbH

RAF1 Control Peptide

RAF1 Purified
50 µg / €190.00
  Acris Antibodies GmbH

RAF1 antibody

RAF1 Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

RAF1 pSer259 antibody

RAF1 Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

RAF1 pSer259 antibody

Immunohistochemistry (IHC) analyzes of Raf1 pSer259 antibody (Cat.-No.: AP01674PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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