RAF1 antibody

Principal name

RAF1 antibody

Alternative names for RAF1 antibody

C-RAF, Raf-1, cRaf, RAF proto-oncogene serine/threonine-protein kinase

SwissProt ID

A7E3S4 (Bovin), P04049 (Human), P05625 (Chick), P09560 (Xenla), P11345 (Rat), Q99N57 (Mouse)

Gene ID

5894 (RAF1), 24703 (Raf1), 110157

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Xen (Xenopus), Por (Porcine)

Available hosts

Rabbit, Mouse, Rat, Chicken

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of RAF1 antibody

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAF1 antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAF1 antibody Cat.-No AM06729PU-N with DAB staining. Mouse IgG1 4G4 Purified Hu E, F, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RAF1 antibody

  Rabbit Anti-Raf-1 (Ser301) Antibody (Phospho-Specific)  
Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf-1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho Ser301 Raf-1 antibody. The immunolabeling of Raf-1 is completely eliminated by treatment with λ-Ptase. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Anti-Raf-1 (Ser642) Antibody (Phospho-Specific)  
RAF1 Rabbit IgG Purified Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit polyclonal Raf1 (Ab-341) antibody  
Western blot analysis of extracts from HT-29 cells, using Raf1 (Ab-341) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit polyclonal Raf1 (Ab-621) antibody  
Western blot analysis of extracts from HeLa cells, treated with UV (5mins), using Raf1 (Ab-621) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit polyclonal C-RAF (Ser642) antibody(Phospho-specific)  
RAF1 Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit polyclonal C-RAF (Thr269) antibody(Phospho-specific)  
RAF1 Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit polyclonal C-RAF (Ab-642) antibody  
Western blot analysis of extracts from CoLo cells, using C-RAF (Ab-642) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Mouse Anti-Human RAF1/RAF Monoclonal Antibody  
Anti-RAF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG1 Purified Hu E
50 µg / €400.00
  OriGene Technologies, Inc.

RAF1 (N-term) antibody

  Chicken Polyclonal C-raf Antibody  
Western blot analysis of C-raf in 3T3 cell lysate with C-raf antibody at (A) 0.5 and (B) 1 ug/mL. Chicken Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Polyclonal C-RAF Antibody  
Western blot analysis of C-RAF expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Polyclonal C-RAF (Ser259) Antibody (Phospho-specific)  
Western blot analysis of C-RAF phosphorylation expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Polyclonal C-RAF (Ser296) Antibody (Phospho-specific)  
Western blot analysis of C-RAF phosphorylation expression in PMA treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Polyclonal C-RAF (Ser338) Antibody (Phospho-specific)  
Western blot analysis of C-RAF phosphorylation expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Polyclonal C-RAF (Ser621) Antibody (Phospho-specific)  
Western blot analysis of C-RAF phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Polyclonal C-RAF Antibody  
Western blot analysis of C-RAF expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Polyclonal Anti-C-RAF Antibody  
Western blot analysis on Jurkat cell lysate using C-RAF Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAF1 antibody

  Rabbit Polyclonal Anti-RAF1 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (RAF1 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAF1 pSer621 antibody

RAF1 Mouse IgG2a 6B4 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RAF1 antibody

Immunoblot Analysis A431 cell lysate probed with mab C-Raf (PBB-1) at 0.1 µg/ml. Mouse IgG1 PBB-1 Purified Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

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