RAG1 / RNF74 antibody

Principal name

RAG1 / RNF74 antibody

Alternative names for RAG1 / RNF74 antibody

RAG-1, V(D)J recombination-activating protein 1, RING finger protein 74

SwissProt ID

O13033 (Danre), P15918 (Human), P15919 (Mouse), P24271 (Chick), P34088 (Rabit), Q867B5 (Pig), Q91829 (Xenla)

Gene ID

5896 (RAG1), 19373, 2958967 (P22882)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of RAG1 / RNF74 antibody

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1.

General readings

"The V(D)J recombination activating gene, RAG-1." Schatz D.G., Oettinger M.A., Baltimore D. Cell 59:1035-1048(1989) [PubMed: 2598259] [Abstract]

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAG1 / RNF74 (C-term) antibody

Mouse Liver; WB Suggested Anti-Rag1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Liver; Rag1 antibody - C-terminal region (AP46008PU-N) in Mouse Liver cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  Acris Antibodies GmbH

RAG1 / RNF74 (C-term) antibody

Mouse Liver; WB Suggested Anti-Rag1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Liver; Rag1 antibody - C-terminal region (ARP42949_P050) in Mouse Liver cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

RAG1 / RNF74 antibody

RAG1 / RNF74 Rabbit Aff - Purified Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAG1 / RNF74

RAG1 / RNF74 Human
2 µg / €300.00
  Abnova Taiwan Corp.

RAG1 / RNF74

RAG1 / RNF74 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RAG1 / RNF74

RAG1 / RNF74 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

RAG1 overexpression lysate

RAG1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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