RAG2 antibody

Principal name

RAG2 antibody

Alternative names for RAG2 antibody

RAG-2, V(D)J recombination-activating protein 2

SwissProt ID

O13034 (Danre), P21784 (Mouse), P25022 (Chick), P34089 (Rabit), P55895 (Human), Q91830 (Xenla)

Gene ID

5897 (RAG2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of RAG2 antibody

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAG2 antibody

Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA307957 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAG2 antibody

Western blot analysis of extracts of various cell lines, using RAG2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAG2 antibody

Western blot analysis using RAG2 mouse mAb against RAG2(AA: 350-527)-hIgGFc transfected HEK293 (1)cell lysate. Mouse IgG1 4D5 Ascites Hu E, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

RAG2 (C-term) antibody

Formalin fixed and paraffin embedded human tonsil tissue stained with RAG2 Antibody (C-term) Cat.-No  AP53570PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAG2 antibody

Immunofluorescence of purified MaxPab antibody to RAG2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RAG2 antibody

RAG2 Rabbit Serum Hu, Rt E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

RAG2 antibody

Western Blot analysis of RAG2 expression in transfected 293T cell line (H00005897-T02) by RAG2 MaxPab polyclonal antibody.

Lane 1: RAG2 transfected lysate(59.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RAG2 antibody

Detection limit for recombinant GST tagged RAG2 is approximately 1ng/ml as a capture antibody. Mouse IgG2b 2G8 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAG2 antibody

Western Blot: RAG2 Antibody (4D5) [NBP1-47482] - Western blot analysis using RAG2 mouse mAb against RAG2-hIgGFc transfected HEK293 (1)cell lysate. Mouse IgG1 4D5 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAG2

RAG2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RAG2

RAG2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RAG2

RAG2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RAG2

RAG2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

RAG2 (Human) IP-WB Antibody Pair

RAG2 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RAG2 293T Cell Transient Overexpression Lysate(Denatured)

RAG2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

RAG2 overexpression lysate

RAG2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

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