RAIDD antibody

Principal name

RAIDD antibody

Alternative names for RAIDD antibody

CRADD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain, RIP-associated protein with a death domain

SwissProt ID

O88843 (Mouse), P78560 (Human)

Gene ID

8738 (CRADD), 12905 (Cradd)

Available reactivities

Hu (Human), Hst (Hamster), Mky (Monkey), Ms (Mouse), Prim (Primate), Bear, Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of RAIDD antibody

Apoptosis is a major form of cell death characterized by severa l morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signaling pathway that leads to the recruitment and activation of caspases, a family of cysteine-containing , aspartate-specific proteases. Adaptor molecules that contai n protein-protein interaction motifs mediate the coupling of caspases to signaling pathway. For example, in the TNF receptor I mediated apoptosis pathway, the apoptotic signal is transduced through interaction of the oligomerized receptor death domain (DD) with a set of cy toplasmic adaptor/signaling molecules including FADD, TRADD and RIP. RAIDD (also known as CRADD) is another candidate adaptor molecule. It has a bipartite architecture comprising a carboxy-terminal DD and an amino-terminal caspase recruitment domain (CARD) highly homologous with the sequence of the prodomain of human caspase-2 and C. elegans CED-3. RAIDD interacts with RIP on respective DD, and with caspase-2 or CED-3 on respective CARD. Thus it may play a role in the TNF-receptor I mediated apoptosis by recruiting caspase-2 to the pathway. However, it has been remained unclea r about the significance of caspase-2 activation through the RIP/RAIDD pathway for death induction at least in some situation, because targeted disruption of RIP in mice, as well as mutational ablation of RIP function in cultured cells did not interfere with death induction by TNF nor by Fas, while NF- κ B activation through RIP was abolished in these cells.

General readings

Park HH., (2006). J Mol Biol. 357(2):358-64.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAIDD (Center) antibody

  Rabbit Polyclonal RAIDD Antibody  
Western blot analysis of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAIDD (C-term) antibody

  Rabbit Polyclonal RAIDD Antibody  
Western blot analysis of RAIDD in HeLa total cell lysate with RAIDD antibody at 1 ug/ml. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAIDD antibody

  Rabbit Polyclonal Anti-CRADD Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (CRADD Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAIDD (full length) antibody

Western blot analysis of RAIDD expression in Jurkat (1), HeLa (2), WR19L (3) and PC12 (4) using AM26605AF-N. Mouse IgG1 4B12 Azide Free Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

RAIDD antibody

Western blot analysis: The Recombinant Human CRADD (50, 100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT14G8 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

RAIDD antibody

Western blot analysis: The Recombinant Human CRADD (50, 100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT14G8 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

RAIDD antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RAIDD (112-126) antibody

Testis, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Elephant, Hst, Hu, Mky, Ms, Prim P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RAIDD (99-117) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human RAIDD (SP1261) Rabbit Purified Hu ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

RAIDD (99-117) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human RAIDD (SP1261) Rabbit IgG Purified Hu ICC/IF, WB
50 µg / €280.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CRADD - human gene knockout kit via CRISPR

CRADD - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CRADD - human gene knockout kit via CRISPR

CRADD - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CRADD - human gene knockout kit via CRISPR

CRADD - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CRADD - human gene knockout kit via CRISPR

CRADD - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cradd - mouse gene knockout kit via CRISPR

Cradd - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cradd - mouse gene knockout kit via CRISPR

Cradd - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cradd - mouse gene knockout kit via CRISPR

Cradd - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cradd - mouse gene knockout kit via CRISPR

Cradd - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAIDD

  Recombinant protein of human CASP2 and RIPK1 domain containing adaptor with death domain (CRADD)  
RAIDD Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAIDD (1-199, His-tag)

RAIDD Human Purified > 95 % >/= 95% by SDS PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

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