RALB antibody

Principal name

RALB antibody

Alternative names for RALB antibody

Ral-B, Ras-related protein Ral-B

SwissProt ID

P11234 (Human), P36860 (Rat), Q9JIW9 (Mouse)

Gene ID

5899 (RALB), 116546 (Ralb)

Available reactivities

Hu (Human), Ms (Mouse), Dros (Drosophila), Mky (Monkey), Rt (Rat), Can (Canine), Bov (Bovine), Eq (Equine), Gt (Goat), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RALB antibody

RalB is a member of the Ras-like GTP binding protein family. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family.These proteins are characterized by a molecular weight between
20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsicGTPase activity. These proteins relay signals from the plasmamembrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene.
Ras and Ras-like proteins function as switches, cycling between 2 distinct conformational states, active when GTP is bound and inactive when GDP is bound. Affinity precipitation assays can be used to determine the activation status of Ras and Ras-like family members. This assay is based on the ability of the Ras family effector proteins to interact specifically with the activated GTP-bound form of their target GTPases.

General readings

1. Zhao, Z. & Rivkees, S.A., Tissue-specific expression of GTPase RalA and RalB during. embryogenesis and regulation by epithelial-mesenchymal interaction. Mech. Dev. 2000, 97,
201-204.
2. Bhullar, R.P. & Yang, S., Immunodetection of ralA and ralB GTP-binding proteins in various rat tissues and platelets. Mol. Cell Biochem. 1998, 179, 49-55.
3. Jilkina, O. & Bhullar, R.P., Generation of antibodies specific for the RalA and RalB GTP-binding proteins and determination of their concentration and distribution in human platelets. Biochim. Biophys. Acta 1996, 1314, 157-166.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RALB antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RALB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505880) Mouse IgG1 OTI2C4 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RALB antibody

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. TA308189 diluted at 1:1000 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RALB antibody

Gel: 10%SDS-PAGE, Lysate: 30 µg, Lane: Mouse lung tissue, Primary antibody: RALB Antibody at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RALB antibody

Predicted band size: 24kDa. Positive control: Mouse lung tissue lysate . Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RALB antibody

WB Suggested Anti-RALB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: ACHN cell lysateRALB is strongly supported by BioGPS gene expression data to be expressed in Human ACHN cells Rabbit IgG Purified Bov, Can, Eq, Gt, Hu, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RALB antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4D1 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

RALB antibody

RALB MaxPab polyclonal antibody. Western Blot analysis of RALB expression in human placenta. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RALB antibody

RALB MaxPab polyclonal antibody. Western Blot analysis of RALB expression in human placenta. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RALB antibody

RALB MaxPab rabbit polyclonal antibody. Western Blot analysis of RALB expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RALB antibody

Immunoperoxidase of monoclonal antibody to RALB on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG2a 4D1 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RALB antibody

RALB Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RALB antibody

Western blot analysis of RALB expression in HepG2 (A); Jurkat (B); A549 (C); NIH3T3 (D); PC12 (E) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RALB antibody

Western blot analysis of RALB expression in HepG2 (A); Jurkat (B); A549 (C); NIH3T3 (D); PC12 (E) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RALB antibody

Western blot analysis of RALB expression in HepG2 (A); Jurkat (B); A549 (C); NIH3T3 (D); PC12 (E) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

RALB antibody

Western Blot: RalB Antibody [NBP1-32696] - (30 ug of whole cell lysate) A: Hep G2  12% SDS PAGE diluted at 1:1000 Rabbit IgG Hu WB
0.1 ml / €420.00
  Novus Biologicals Inc.

RALB antibody

RALB Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RALB

RALB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

RALB

RALB Human Purified
25 µg / €400.00
  Acris Antibodies GmbH

RALB (1-203, His-tag)

RALB Human Purified > 90 % E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

RALB (1-203, His-tag)

RALB Human Purified > 90 % E. coli
50 µg / €370.00
  Acris Antibodies GmbH

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