RAN antibody

Principal name

RAN antibody

Alternative names for RAN antibody

GTPase Ran, ARA24, GTP-binding nuclear protein Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24

SwissProt ID

O17915 (Caeel), P42558 (Chick), P52301 (Xenla), P62825 (Canfa), P62826 (Human), P62827 (Mouse), P62828 (Rat), P79735 (Danre), Q3T054 (Bovin), Q9CWI7 (Human), Q9UEU9 (Canfa), Q9VZ23 (Drome)

Gene ID

5901 (RAN), 6330572 (def)

Available reactivities

Bov (Bovine), Hst (Hamster), Hu (Human), Ms (Mouse), Xen (Xenopus), Rt (Rat), Can (Canine), Ce (C. elegans), Dros (Drosophila), Ze (Zebrafish), Ye (Yeast), Mky (Monkey), Eq (Equine), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of RAN antibody

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq]

General readings

General / background references:Wente SR Gatekeepers of the nucleus. Science 288:1374-7 (2000). PubMed PMID: 10827939
Nakielny S & Dreyfuss G Transport of proteins and RNAs in and out of the nucleus. Cell 99:677-90 (1999). PubMed PMID: 10619422
Hieda M et al. A monoclonal antibody to the COOH-terminal acidic portion of Ran inhibits both the recycling of Ran and nuclear protein import in living cells. J Cell Biol 144:645-55 (1999). PubMed PMID: 10037787
Avis JM & Clarke PR Ran, a GTPase involved in nuclear processes: its regulators and effectors. J Cell Sci 109 ( Pt 10):2423-7 (1996). PubMed PMID: 8923203

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAN antibody

Western blot analysis of extracts of various cell lines, using RAN antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAN (197-216) antibody

Human breast stained with Anti-Ran, cat# AP15754PU. Rabbit Purified Hu, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

RAN (197-216) antibody

Human breast stained with Anti-Ran, cat# AP15754PU. Rabbit Purified Hu, Ms, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

RAN (197-216) antibody

Human breast stained with Anti-Ran, cat# AP15754PU. Rabbit Purified Hu, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

RAN (N-term) antibody

TA302950 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

RAN (N-term) antibody

TA302950 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAN antibody

Western blot analysis of HepG2 cell lysates with anti-RAN antibody (1:200). This antibody identified a band of ~23kDa in HepG2 cell lysates. Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAN antibody

Predicted band size: 25kDa. Positive control: Hela, NIH/3T3 and HepG2 cell, Mouse testis tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: NIH/3T3 cell lysate Lane3: HepG2 cell lysate Lane4: Mouse testis tissue lysate Lysates: 30 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAN antibody

Predicted band size: 25kDa. Positive control: Hela, NIH/3T3 and HepG2 cell, Mouse testis tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: NIH/3T3 cell lysate Lane3: HepG2 cell lysate Lane4: Mouse testis tissue lysate Lysates: 30 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAN antibody

WB Suggested Anti-RAN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAN antibody

Western blot analysis of RAN expression in LOVO cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAN (197-210) antibody

TA348979 (1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAN antibody

Immunocytochemical detecton of Ran on 4% PFA fixed HeLa cells with AM26498AF-N. Mouse IgG2b ARAN1 Azide Free Hst, Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

RAN antibody

Immunohistochemistry(IHC) analyzes of RAN antibody (Cat.-No.: AP06300PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RAN (N-term) antibody

(LEFT) Western blot analysis of RAN Antibody (N-term) (AP17702PU-N) in HL-60 cell line lysates (35ug/lane).RAN (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of RAN antibody (N-term) (AP17702PU-N) in NIH-3T3 cell line lysates (35ug/lane). RAN (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RAN antibody

RAN polyclonal antibody (A01), Lot # 050921JC01 Western Blot analysis of RAN expression in Hela NE ( Cat # L013V3 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RAN antibody

RAN polyclonal antibody ( Cat # PAB6653 ) staining ( 1 µg/ml ) of A-431 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

RAN antibody

Immunohistochemical staining of RAN on formalin fixed, paraffin embedded human breast with RAN polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

RAN antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RAN Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

RAN antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RAN Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

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