RANBP1 antibody

Principal name

RANBP1 antibody

Alternative names for RANBP1 antibody

Ran-binding protein 1, Ran-specific GTPase-activating protein

SwissProt ID

P34022 (Mouse), P43487 (Human), Q3T0M7 (Bovin)

Gene ID

5902 (RANBP1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Eq (Equine), Ye (Yeast)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of RANBP1 antibody

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. [provided by RefSeq]

General readings

Bischoff FR et al. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J 14:705-15 (1995). PubMed PMID: 7882974

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RANBP1 (C-term) antibody

TA302686 (0.05µg/ml) staining of HELA lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence. Goat Aff - Purified Hu E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

RANBP1 (C-term) antibody

TA302686 (0.05µg/ml) staining of HELA lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RANBP1 (1-187) antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308769 diluted at 1:1000. Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RANBP1 antibody

RANBP1 Rabbit IgG Purified Bov, Eq, Hu, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

RANBP1 antibody

RANBP1 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RANBP1 antibody

RANBP1 polyclonal antibody ( Cat # PAB6654 ) staining ( 0.1 µg/ml ) of HepG2 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

RANBP1 antibody

Human 721_B; WB Suggested Anti-RANBP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 721_B cell lysate. RANBP1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; RANBP1 antibody - C-terminal region (ARP46083_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RANBP1 antibody

Western blot analysis of RANBP1 expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RANBP1 antibody

Western blot analysis of RANBP1 expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RANBP1 antibody

Western blot analysis of RANBP1 expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

RANBP1 antibody

NB 100-1443 staining (0.1µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

RANBP1 antibody

WB and IP: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody BL1968 NB100-79815 at 5 mcg/0.5 mg lysate. Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

RANBP1 antibody

Immunoprecipitation: RanBP1 Antibody [NB100-79815] - Detection of Human RanBP1 by Immunoprecipitation  followed by Western Blot. Samples: Whole cell lysate (0.5 mg)  from HeLa cells. Antibodies: Affinity purified rabbit anti-  RanBP1 antibody BL1968 (Cat. No. NB100-79815) used at 5  mcg/0.5 mg lysate for IP. RanBP1 was also immunoprecipitated  using rabbit anti-RanBP1 antibody BL1966 (Cat. No.  NB100-79814) at 5 mcg/0.5 mg lysate. Immunoprecipitated  RanBP1 was blotted using NB100-79814 at 1 mcg/ml. Detection:  Chemiluminescence with an exposure time of 5 minutes (A and  B).  25043 West Rabbit IgG Aff - Purified Hu IP
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RANBP1

RANBP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RANBP1

RANBP1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

RANBP1 (full length, N-term HIS tag)

RANBP1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

RANBP1 (1-201, His-tag)

RANBP1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

RANBP1 (1-201, His-tag)

RANBP1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

RANBP1

RANBP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RANBP1

RANBP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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