Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken)
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP)
Background of RanBP13 / Importin-13 antibody
**Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application. Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. Expressed in fetal brain, heart, intestine and kidney. Belongs to the importin beta family. Contains 14 HEAT repeats. Contains 1 importin N-terminal domain.
Immunohistochemical of paraffin-embedded human gliomas using 11696-2-AP(IPO13 antibody) at dilution of 1:50 (under 10x lens)
Western Blot: Importin-13 Antibody [NBP1-31508] - Western Blot analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody, diluted at 1:500._x000d_A: Non-transfected lysate. _x000d_B: IPO13 transfected lysate.
Immunoprecipitation: Importin-13 Antibody [NBP1-31508] - IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-NBP1-31508. The precipitated IPO13 was detected by NBP1-31508 diluted at 1:1000.
Importin 13 antibody detects Importin 13 protein by western blot analysis. Non-transfected (-) and Importin 13-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Importin 13 antibody (GTX115274) diluted by 1:1000.