Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Ze (Zebrafish)
Goat, Mouse, Rabbit
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of RanBP7 / Importin-7 antibody
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq]
Gorlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, Prehn S, Izaurralde E.A novel class of RanGTP binding proteins.J Cell Biol 1997 Jul 14;138(1):65-80 PMID: 9214382
GTX15840 staining (0.1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IPO7 polyclonal antibody ( Cat # PAB6218 ) staining ( 0.1 µg/ml ) of human brain lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:500.
Importin 7 antibody [C3], C-term detects IPO7 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IPO7 protein stained by Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:500.Blue: Hoechst 33343 staining.
Western Blot: Importin-7 Antibody [NBP1-46167]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Importin 7 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Importin 7 was also immunoprecipitated by rabbit anti-Importin 7 antibody NBP1-46168, which recognizes a downstream epitope.
Western Blot: Importin-7 Antibody [NBP1-46168]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-Importin 7 antibody used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Importin 7 was also immunoprecipitated by rabbit anti-Importin 7 antibody NBP1-46167, which recognizes an upstream epitope.