RANTES / CCL5 antibody

Principal name

RANTES / CCL5 antibody

Alternative names for RANTES / CCL5 antibody

C-C motif chemokine 5, D17S136E, SCYA5, Small-inducible cytokine A5, SIS-delta, EoCP, T-cell-specific protein RANTES, cell-specific protein P228, Eosinophil-chemotactic cytokine

SwissProt ID

O97919 (Bovin), P13501 (Human), P30882 (Mouse), P50231 (Rat), Q29288 (Pig)

Gene ID

20304 (Ccl5), 6352 (CCL5), 4506847, 81780

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Rat, Goat, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT)

Background of RANTES / CCL5 antibody

RANTES is a member of the beta (C-C) chemokine subfamily, which also includes the human proteins MCP1, MCP2, MIP1 alpha, MIP1 beta, I309, and their murine counterparts. Mouse RANTES cDNA encodes a 91 amino acid residue precursor polypeptide with a 23 amino acid residue hydrophobic signal peptide that is cleaved to produce the 68 amino acid residue mature protein (approximately 7.9 kDa). Human and mouse RANTES share approximately 85 % amino acid identity. RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) was first discovered by subtractive hybridization as a transcript expressed in T cells but not B cells. Eosinophillic chemotactic substances released by thrombin stimulated human platelets is identical to RANTES. In addition to T cells and platelets, RANTES is expressed by renal tubular epithelium , synovial fibroblasts, and selected tumor cells (rhabdomyosarcoma cells and MG63 osteosarcoma cells). The mouse RANTES gene maps to chromosome 11. The chemokine RANTES is a chemoattractant for monocytes, T lymphocytes , and NK (natural killer) cells. RANTES selectively attracts T cells of the CD4+/CD45RO+ phenotype in vitro. RANTES can also chemoattract and degranulate eosinophils , as well as chemoattract and induce histamine release from basophils. RANTES has been shown to inhibit HIV-1 entry via human CCR5. RANTES and MIP alpha bind to chemokine receptors (CCR1, CCR4, and CCR5) and induce activation of STAT signaling pathways. Because of its in vitro biological activities, RANTES may play an important role in mediating immune and inflammatory responses.

General readings

General / background references:Bleul CC et al. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature 382:829-33 (1996). PubMed PMID: 8752280
Tashiro K et al. Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science 261:600-3 (1993). PubMed PMID: 8342023
Schall TJ et al. Molecular cloning and expression of the murine RANTES cytokine: structural and functional conservation between mouse and man. Eur J Immunol 22:1477-81 (1992). PubMed PMID: 1376260
Schall TJ et al. Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine RANTES. Nature 347:669-71 (1990). PubMed PMID: 1699135

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RANTES / CCL5 antibody

Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Mouse IgG2b 8.PP.11 Azide Free Hu E, NEUT, P
50 µg / €345.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

Immunoblot with Anti-Mouse CCL5 (RANTES) Purified.  Raw 264.7 cells were treated with Brefeldin A (lane 1) or LPS and Brefeldin A (lane 2) for 16 hours prior to lysis. Mouse R6G9 Purified Ms WB
25 µg / €120.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

To detect hRANTES by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hRANTES. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

To detect hRANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human RANTES (TA328431) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

To detect mRANTES by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mRANTES. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

To detect mRANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RANTES (TA328548) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRANTES. Rabbit IgG Purified E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

To detect Rat RANTES by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat RANTES. Rabbit IgG Purified Rt E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

To detect Rat RANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat RANTES (TA328592) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat RANTES. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

Western blot analysis of extracts of various cell lines, using CCL5 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

WB Suggested Anti-CCL5 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RANTES / CCL5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCL5 (RC203799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCL5. Mouse IgG1 OTI2A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RANTES / CCL5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCL5 (RC203799, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCL5. Mouse IgG1 OTI10G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RANTES / CCL5 antibody

Immunohistochemistry analysis of RANTES Antibody (Cat.-No AP20618PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RANTES / CCL5 antibody

RANTES / CCL5 Rabbit Biotin Rt E, WB
25 µg / €230.00
  Acris Antibodies GmbH

RANTES / CCL5 antibody

RANTES / CCL5 Rabbit Biotin Rt E, WB
50 µg / €340.00
  Acris Antibodies GmbH

RANTES / CCL5 antibody

RANTES / CCL5 Rabbit Aff - Purified Rt E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

RANTES / CCL5 antibody

RANTES / CCL5 Rabbit Aff - Purified Rt E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

RANTES / CCL5 antibody

RANTES / CCL5 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

RANTES / CCL5 antibody

RANTES / CCL5 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

RANTES / CCL5 antibody

Immunohistochemistry: The RANTES antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.0 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an AEC chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

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