RAPGEF3 antibody

Principal name

RAPGEF3 antibody

Alternative names for RAPGEF3 antibody

CGEF1, EPAC, EPAC1, EPAC 1, cAMP-GEFI, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, Rap1 guanine-nucleotide-exchange factor directly activated by cAMP, cAMP-regulated guanine nucleotide exchange factor I

SwissProt ID

O95398 (Human), Q8VCC8 (Mouse), Q9Z1C8 (Rat)

Gene ID

10411

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of RAPGEF3 antibody

General secondary messenger cAMP levels are altered by many extracellular physiological signals including neurotransmitters, immunomodulators and hormones via the activation of G-protein coupled receptor (GPCR) activation. Activation of GPCRs by ligand interaction stimulates the heterotrimeric G-proteins thereby increasing the activity of one or more subtypes of adenylate cyclases to increase the formation of cAMP. The increased intracellular levels of cAMP transduce the signal transduction via protein kinase A and via small GTPase, RAP 1 (1,2). The activation of RaP1 by cAMP is independent f PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represent a novel mechanism for cAMP signaling with in the cAMP cascade.
There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors (3). EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity [2]. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands (4, 5). Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex (6).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAPGEF3 antibody

Western blot analysis of extracts from Jurkat and HeLa cells, using RAPGEF3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RAPGEF3 antibody

Western blot analysis of extracts of various cell lines, using RAPGEF3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAPGEF3 antibody

WB Suggested Anti-RAPGEF3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAPGEF3 antibody

Human CFBE Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAPGEF3 control peptide

RAPGEF3
0.25 mg / €250.00
  Acris Antibodies GmbH

RAPGEF3 (N-term) antibody

RAPGEF3 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

RAPGEF3 control peptide

RAPGEF3
0.25 mg / €250.00
  Acris Antibodies GmbH

RAPGEF3 (526-541) antibody

RAPGEF3 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

RAPGEF3 antibody

RAPGEF3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RAPGEF3 antibody

RAPGEF3 Rabbit Serum Hu, Ms IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

RAPGEF3 antibody

Western Blot analysis of RAPGEF3 expression in transfected 293T cell line (H00010411-T02) by RAPGEF3 MaxPab polyclonal antibody.

Lane 1: RAPGEF3 transfected lysate(99.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RAPGEF3 antibody

RAPGEF3 monoclonal antibody (M01), clone 2E5. Western Blot analysis of RAPGEF3 expression in A-431 ( Cat # L015V1 ). Mouse IgG2a 2E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAPGEF3 (N-term) antibody

Human COLO205; WB Suggested Anti-RAPGEF3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate; RAPGEF3 antibody - N-terminal region (ARP52139_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RAPGEF3 (Center) antibody

Human CFBE; RAPGEF3 antibody - middle region (ARP52140_P050) in Human CFBE cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RAPGEF3 antibody

Immunofluorescent analysis of EPAC1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RAPGEF3 antibody

Immunofluorescent analysis of EPAC1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RAPGEF3 antibody

Immunofluorescent analysis of EPAC1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

RAPGEF3 antibody

Western Blot: RAPGEF3 Antibody [NBP1-57044] - This Anti-RAPGEF3 antibody was used in Western Blot of COLO205       tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

RAPGEF3 antibody

RAPGEF3 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

RAPGEF3 antibody

Immunohistochemistry-Paraffin: RAPGEF3 Antibody (EPR1672) [NBP1-95559] human kidney tissue using Anti-Epac1. Rabbit EPR1672 Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

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