RAPGEF3 antibody

Principal name

RAPGEF3 antibody

Alternative names for RAPGEF3 antibody

CGEF1, EPAC, EPAC1, EPAC 1, cAMP-GEFI, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, Rap1 guanine-nucleotide-exchange factor directly activated by cAMP, cAMP-regulated guanine nucleotide exchange factor I

SwissProt ID

O95398 (Human), Q8VCC8 (Mouse), Q9Z1C8 (Rat)

Gene ID

10411 (RAPGEF3), 223864 (Rapgef3), 59326

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RAPGEF3 antibody

General secondary messenger cAMP levels are altered by many extracellular physiological signals including neurotransmitters, immunomodulators and hormones via the activation of G-protein coupled receptor (GPCR) activation. Activation of GPCRs by ligand interaction stimulates the heterotrimeric G-proteins thereby increasing the activity of one or more subtypes of adenylate cyclases to increase the formation of cAMP. The increased intracellular levels of cAMP transduce the signal transduction via protein kinase A and via small GTPase, RAP 1 (1,2). The activation of RaP1 by cAMP is independent f PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represent a novel mechanism for cAMP signaling with in the cAMP cascade.
There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors (3). EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity [2]. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands (4, 5). Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex (6).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAPGEF3 antibody

  Rabbit anti-RAPGEF3 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using RAPGEF3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAPGEF3 antibody

  Rabbit Polyclonal Anti-RAPGEF3 Antibody  
WB Suggested Anti-RAPGEF3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RAPGEF3 antibody

  Rabbit Polyclonal Anti-RAPGEF3 Antibody  
Human CFBE Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

Rapgef3 - mouse gene knockout kit via CRISPR

Rapgef3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rapgef3 - mouse gene knockout kit via CRISPR

Rapgef3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rapgef3 - mouse gene knockout kit via CRISPR

Rapgef3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rapgef3 - mouse gene knockout kit via CRISPR

Rapgef3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAPGEF3 (transcript variant 2)

  Recombinant protein of human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2  
RAPGEF3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAPGEF3 (transcript variant 3)

  Recombinant protein of human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 3  
RAPGEF3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RAPGEF3 (mGFP-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, RAPGEF3 (mGFP-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, RAPGEF3 (mGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, RAPGEF3 (mGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, RAPGEF3 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, RAPGEF3 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, RAPGEF3 (Myc-DDK tagged) - Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 3 , 200 uL, >10^7 TU/mL

Lenti ORF particles, RAPGEF3 (Myc-DDK tagged) - Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 3 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rapgef3 (GFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rapgef3 (GFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,680.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rapgef3 (GFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rapgef3 (GFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,670.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rapgef3 (GFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rapgef3 (GFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,660.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,510.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,500.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rapgef3 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,490.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rapgef3 (GFP-tagged ORF) - Rat Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rapgef3 (GFP-tagged ORF) - Rat Rap guanine nucleotide exchange factor (GEF) 3 (Rapgef3), 200 uL, >10^7 TU/mL  
200 µl / €1,630.00
  OriGene Technologies, Inc.

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