RAPGEF4 antibody

Principal name

RAPGEF4 antibody

Alternative names for RAPGEF4 antibody

CGEF2, EPAC2, EPAC 2, cAMP-GEFII, Rap guanine nucleotide exchange factor 4, Exchange factor directly activated by cAMP 2, Exchange protein directly activated by cAMP 2, cAMP-regulated guanine nucleotide exchange factor II

SwissProt ID

Q8WZA2 (Human), Q9EQZ6 (Mouse), Q9Z1C7 (Rat)

Gene ID

11069 (RAPGEF4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of RAPGEF4 antibody

General secondary messenger cAMP levels are altered by many extracellular physiological signals including neurotransmitters, immunomodulators and hormones via the activation of G-protein coupled receptor (GPCR) activation. Activation of GPCRs by ligand interaction stimulates the heterotrimeric G-proteins thereby increasing the activity of one or more subtypes of adenylate cyclases to increase the formation of cAMP. The increased intracellular levels of cAMP transduce the signal transduction via protein kinase A and via small GTPase, RAP 1 (1,2). The activation of RaP1 by cAMP is independent f PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represent a novel mechanism for cAMP signaling with in the cAMP cascade.
There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors (3). EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity [2]. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands (4, 5). Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex (6).

General readings

Ueno H, Shibasaki T, Iwanaga T, et al. Characterization of the gene EPAC2: structure, chromosomal localization, tissue expression, and identification of the liver-specific isoform. Genomics 2001; 78:91-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAPGEF4 (N-term) antibody

Western blot analysis of EPAC2 in rat liver tissue lysate with EPAC2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAPGEF4 control peptide

RAPGEF4
0.25 mg / €250.00
  Acris Antibodies GmbH

RAPGEF4 (743-761) antibody

RAPGEF4 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

RAPGEF4 (N-term) Control Peptide

Western blot analysis in rat liver tissue lysate with RAPGEF4 / EPAC2 antibody Cat.-No. AP55604PU-N at1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
50 µg / €160.00
  Acris Antibodies GmbH

RAPGEF4 antibody

Western Blot analysis of RAPGEF4 expression in transfected 293T cell line (H00011069-T01) by RAPGEF4 MaxPab polyclonal antibody.

Lane 1: RAPGEF4 transfected lysate(111.21 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RAPGEF4 antibody

RAPGEF4 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RAPGEF4 antibody

Detection limit for recombinant GST tagged RAPGEF4 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2b 1C11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RAPGEF4 (transcript variant 1)

RAPGEF4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAPGEF4

RAPGEF4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RAPGEF4

RAPGEF4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RAPGEF4

RAPGEF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RAPGEF4

RAPGEF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RAPGEF4 293T Cell Transient Overexpression Lysate(Denatured)

RAPGEF4 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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