RAPTOR antibody

Principal name

RAPTOR antibody

Alternative names for RAPTOR antibody

RPTOR, KIAA1303, Regulatory-associated protein of mTOR, P150 target of rapamycin TOR-scaffold protein

SwissProt ID

Q55BR7 (Dicdi), Q8K4Q0 (Mouse), Q8N122 (Human)

Gene ID

57521 (RPTOR), 74370

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Dot blot (Dot), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of RAPTOR antibody

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 2). Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation (3). These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels (4). Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins (5).

General readings

Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (human)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAPTOR (C-term) antibody

Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 ug/ml. Rabbit IgG Aff - Purified Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAPTOR (N-term) antibody

Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAPTOR (Center) antibody

Western blot analysis of Raptor in L1210 cell lysate with Raptor (IN) antibody at (A) 2 and (B) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RAPTOR antibody

293T cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Raptor antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6232 Purified Hu P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

RAPTOR antibody

Western Blot: Raptor Antibody [TA337071] - Western blot analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000. Rabbit Purified Hu, Ms P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

RAPTOR (incl. pos. control) antibody

Detection of endogenous Raptor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20221PU-N Raptor antibody (Clone 10E10) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: HeLa 
Lane 2: HepG2 
Lane 3: HEK293
Lane 4: SH-SY5Y 
Lane 5: MDCK 
Lane 6: PC12
Lane 7: CMT-93 
Lane 8: Neuro 2A 
Lane 9: 3T3 Mouse IgG1 10E10 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RAPTOR antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

RAPTOR antibody

AP07764PU-N Raptor antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cortex. Rabbit Aff - Purified ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RAPTOR pSer863 antibody

Dot blot analysis of anti-raptor-pSer863 Pab (Cat#AP12910PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

RAPTOR (N-term) antibody

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RAPTOR Control Peptide

RAPTOR
50 µg / €160.00
  Acris Antibodies GmbH

RAPTOR Control Peptide

RAPTOR
50 µg / €160.00
  Acris Antibodies GmbH

RAPTOR Control Peptide

RAPTOR
50 µg / €160.00
  Acris Antibodies GmbH

RAPTOR antibody

Immunofluorescence of purified MaxPab antibody to RPTOR on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RAPTOR antibody

Immunoprecipitation of RPTOR transfected lysate using anti-RPTOR MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RPTOR MaxPab mouse polyclonal antibody (B01) (H00057521-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

RAPTOR antibody

RAPTOR Mouse IgG2a 3C2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

RAPTOR pSer863 antibody

Dot blot analysis of KIAA1303 (phospho S863) polyclonal antibody ( Cat # PAB1647 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml. Rabbit Hu Dot, E
0.4 ml / €400.00
  Abnova Taiwan Corp.

RAPTOR antibody

Immunocytochemistry of Rptor in L1210 cells with Rptor polyclonal antibody ( Cat # PAB13417 ) at 10 µg/ml. Rabbit Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

RAPTOR antibody

Immunocytochemistry of RPTOR in L1210 cells with RPTOR polyclonal antibody ( Cat # PAB13418 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

RAPTOR antibody

Immunocytochemistry staining of L1210 cells using RPTOR polyclonal antibody ( Cat # PAB13419 ) at 1µg/ml Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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