RASA1 / RasGAP antibody

Principal name

RASA1 / RasGAP antibody

Alternative names for RASA1 / RasGAP antibody

Ras GTPase-activating protein 1, GAP, Ras p21 protein activator, p120GAP, RASA, p120RASGAP

SwissProt ID

P09851 (Bovin), P20936 (Human), P50904 (Rat)

Gene ID

5921 (RASA1)

Available reactivities

Bov (Bovine), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Av (Birds)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection))

Background of RASA1 / RasGAP antibody

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq]. Transcript Variant: This variant (1) lacks the alternatively spliced insert found in variant 2, resulting in translation of the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

General / background references:Hu KQ & Settleman J Tandem SH2 binding sites mediate the RasGAP-RhoGAP interaction: a conformational mechanism for SH3 domain regulation. EMBO J 16:473-83 (1997). PubMed PMID: 9034330
Foster R et al. p190 RhoGAP, the major RasGAP-associated protein, binds GTP directly. Mol Cell Biol 14:7173-81 (1994). PubMed PMID: 7935432
Settleman J et al. Association between GTPase activators for Rho and Ras families. Nature 359:153-4 (1992). PubMed PMID: 1522900

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RASA1 / RasGAP antibody

Anti-RASA1 mouse monoclonal antibody (TA505899) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASA1(RC205473). Mouse IgG1 OTI1H2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASA1 / RasGAP antibody

Anti-RASA1 mouse monoclonal antibody (TA505900) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASA1(RC205473). Mouse IgG1 OTI2H1 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RASA1 / RasGAP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASA1 (RC205473, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASA1. Mouse IgG1 OTI1A4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASA1 / RasGAP antibody

Anti-RASA1 mouse monoclonal antibody (TA505935) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASA1(RC205473). Mouse IgG1 OTI4E2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASA1 / RasGAP control peptide

RASA1 / RasGAP Purified WB
50 µg / €190.00
  Acris Antibodies GmbH

RASA1 / RasGAP antibody

Western blot analysis of Ras GAP with lysates from Jutkat cells (Lane 1), mouse small intestine (Lane 2) and rat kidney (Lane 3). Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

RASA1 / RasGAP (C-term) antibody

Western blot analysis of RASA1 / RasGAP (C-term) Cat.-No AP53583PU-N in 293 cell line lysates (35ug/lane). This demonstrates the RASA1 antibody detected the RASA1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RASA1 / RasGAP antibody

RASA1 / RasGAP Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

Western Blot analysis of RASA1 expression in transfected 293T cell line (H00005921-T01) by RASA1 MaxPab polyclonal antibody.

Lane 1: RASA1 transfected lysate(95.7 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

RASA1 / RasGAP Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

Immunoprecipitation of RASA1 transfected lysate using anti-RASA1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RASA1 MaxPab mouse polyclonal antibody (B01) (H00005921-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

Immunoperoxidase of monoclonal antibody to RASA1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 6 ug/ml] Mouse IgG1 2C12 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

RASA1 / RasGAP Mouse IgG1 2C12 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

RASA1 / RasGAP Mouse IgG1 1E6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

RASA1 / RasGAP Mouse IgG1 3D4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

Western blot analysis of RASA1 with lysates from Jutkat cells ( Lane 1 ) , mouse small intestine ( Lane 2 ) and rat kidney ( Lane 3 ) . Rabbit Bov, Hu, Ms, Rt C, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

RASA1 / RasGAP antibody

Immunohistochemistry: RasGAP Antibody [NB100-80043] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-RasGap Rabbit Polyclonal antibody Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

RASA1 / RasGAP antibody

RASA1 / RasGAP Mouse IgG B4F8 Purified Bov, Hu, Ms, Rt ICC/IF, IP, WB
50 µg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RASA1 / RasGAP (transcript variant 1)

RASA1 / RasGAP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RASA1 / RasGAP

RASA1 / RasGAP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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