RASSF1 antibody

Principal name

RASSF1 antibody

Alternative names for RASSF1 antibody

RASSF1A, RDA32, REH3P21, NORE2A, Ras association domain-containing protein 1

SwissProt ID

Q99MK9 (Mouse), Q9NS22 (Human), Q9NS23 (Human)

Gene ID

11186 (RASSF1), 56289 (Rassf1), 28844

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of RASSF1 antibody

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Potential tumor suppressor. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. More specifically, RASSF1a inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. It also inhibits the activation of STK3 and STK4 and may play an additional role as a scaffold protein, directing these enzymes to sites of activation. Isoform C has been shown not to perform these roles, and no function has been identified for this isoform.

General readings

Agathanggelou A, et al. (2005) Cancer Res. 65, 3497-3508
Lerman MI. et. al. (2000) Cancer Res. 60, 6116-6133
Strunnikova M. et. al. (2005) Mol Cell Biol. 25, 3923-3933
Dammann R. et. al. (2005) Histol Histopathol. 20, 645-663
Rossa W.K, et al. (2007) American Journal of pathology. 170, 941-950

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RASSF1 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502487) Mouse IgG1 OTI8E4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASSF1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RASSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502449) Mouse IgG1 OTI2B11 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RASSF1 antibody

Sample(30 ug whole cell lysate). A:293T. 12% SDS PAGE. TA308998diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RASSF1 antibody

RASSF1 Mouse eB114-10H1 Purified Hu IP, WB
25 µg / €120.00
  OriGene Technologies, Inc.

RASSF1 antibody

Predicted band size: 39 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RASSF1 antibody

Predicted band size: 39 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RASSF1 antibody

Western blot analysis of 293T cell lysate using RASSF1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RASSF1 antibody

Anti-RASSF1 mouse monoclonal antibody (TA502480) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1(RC213525). Mouse IgG1 OTI3c2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASSF1 antibody

Anti-RASSF1 mouse monoclonal antibody (TA502481) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1(RC213525). Mouse IgG1 OTI2A9 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASSF1 antibody

Anti-RASSF1 mouse monoclonal antibody (TA502486) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1(RC213525). Mouse IgG2b OTI8E2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASSF1 antibody

Anti-RASSF1 mouse monoclonal antibody (TA502510) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1(RC213525). Mouse IgG1 OTI9F5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASSF1 antibody

Anti-RASSF1 mouse monoclonal antibody (TA502511) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1(RC213525). Mouse IgG1 OTI9A5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASSF1 antibody

Anti-RASSF1 mouse monoclonal antibody (TA502512) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1(RC213525). Mouse IgG1 OTI8A11 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RASSF1 (135-147) antibody

Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

RASSF1 (Isoform A) antibody

Immunocytochemistry: A431 cells were labeled with anti-RASSF1a antibody (clone 3F3) and detection was using a biotinylated secondary antibody and Texas-red conjugated Streptavidin. Mouse IgG1 3F3 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

RASSF1 (Isoform A) antibody

Immunocytochemistry: A431 cells were labeled with anti-RASSF1a antibody (clone 3F3) and detection was using a biotinylated secondary antibody and Texas-red conjugated Streptavidin. Mouse IgG1 3F3 Purified Hu E, ICC/IF, WB

Regular Price: 50 µl / €230.00

Special Price: 50 µl / €161.00

  Acris Antibodies GmbH

RASSF1 antibody

RASSF1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RASSF1 antibody

Immunocytochemistry of A - 431 cells were labeled with RASSF1 monoclonal antibody , clone 3F3 ( Cat # MAB1056 ) , and detection was using a biotinylated secondary antibody and Texas - red conj streptavidin. Mouse IgG1 3F3 Hu E, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

RASSF1 antibody

RASSF1 Goat Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

RASSF1 (C-term) antibody

RASSF1 Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

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