RBCK1 / RNF54 antibody

Principal name

RBCK1 / RNF54 antibody

Alternative names for RBCK1 / RNF54 antibody

RING finger protein 54, C20orf18, UBCE7IP3, XAP3, XAP4, RanBP-type and C3HC4-type zinc finger-containing protein 1, Ubiquitin-conjugating enzyme 7-interacting protein 3, Hepatitis B virus X-associated protein 4, HBV-associated factor 4

SwissProt ID

Q62921 (Rat), Q9BYM8 (Human), Q9WUB0 (Mouse)

Gene ID

10616 (RBCK1), 24105 (Rbck1), 60383

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of RBCK1 / RNF54 antibody

Kit Component:
- KN203906G1, C20orf18 gRNA vector 1 in pCas-Guide vector
- KN203906G2, C20orf18 gRNA vector 2 in pCas-Guide vector
- KN203906D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Yoshimoto,N., (2005) Biochem. Biophys. Res. Commun. 335 (2), 550-557

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RBCK1 / RNF54 (N-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RBCK1 / RNF54 antibody

Western Blot analysis of RBCK1 expression in transfected 293T cell line (H00010616-T01) by RBCK1 MaxPab polyclonal antibody.

Lane 1: C20orf18 transfected lysate(25.3 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RBCK1 / RNF54 antibody

Western Blot analysis of RBCK1 expression in transfected 293T cell line (H00010616-T01) by RBCK1 MaxPab polyclonal antibody.

Lane 1: C20orf18 transfected lysate(25.3 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RBCK1 / RNF54 antibody

Western Blot analysis of RBCK1 expression in transfected 293T cell line (H00010616-T02) by RBCK1 MaxPab polyclonal antibody.

Lane 1: RBCK1 transfected lysate(25.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

RBCK1 / RNF54 antibody

RBCK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of RBCK1 expression in human stomach. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RBCK1 / RNF54 antibody

Western Blot analysis of C20orf18 expression in transfected 293T cell line by C20orf18 monoclonal antibody (M01), clone 3C3.

Lane 1: C20orf18 transfected lysate(25.7 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 3C3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RBCK1 / RNF54 antibody

RBCK1 / RNF54 Mouse IgG2a 1B7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

RBCK1 / RNF54 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with RBCK1 polyclonal antibody ( Cat # PAB1765 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

RBCK1 / RNF54 antibody

Human Jurkat; WB Suggested Anti-C20orf18 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate. RBCK1 is supported by BioGPS gene expression data to be expressed in Jurkat.; C20orf18 antibody - middle region (ARP43073_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

RBCK1 - human gene knockout kit via CRISPR

RBCK1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RBCK1 - human gene knockout kit via CRISPR

RBCK1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RBCK1 - human gene knockout kit via CRISPR

RBCK1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RBCK1 - human gene knockout kit via CRISPR

RBCK1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RBCK1 / RNF54 (transcript variant 2)

RBCK1 / RNF54 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RBCK1 / RNF54

RBCK1 / RNF54 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RBCK1 / RNF54

RBCK1 / RNF54 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RBCK1 / RNF54

RBCK1 / RNF54 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RBCK1 / RNF54

RBCK1 / RNF54 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

C20orf18 293T Cell Transient Overexpression Lysate(Denatured)

C20orf18 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

RBCK1 overexpression lysate

RBCK1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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