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RBFOX3 / NeuN antibody

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Principal name

RBFOX3 / NeuN antibody

Alternative names for RBFOX3 / NeuN antibody

Fox-1 homolog C, FOX3, RNA binding protein fox-1 homolog 3

SwissProt ID

A6NFN3 (Human), Q0VD23 (Bovin), Q8BIF2 (Mouse)

Gene ID

146713 (RBFOX3), 52897 (Rbfox3)

Ncbi ID

NP_001076044.1, NP_001076044, NM_001082575, BC054403, NM_001039167, NM_001039168, NM_001024931, NP_001020102.2

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Chicken, Rabbit

Available applications

Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of RBFOX3 / NeuN antibody

NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples.
Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro.
Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

AM10122SU-N - RBFOX3 / NeuN
Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with NeuN antibody Cat.-No. AM10122SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of granular neuronal cells.
AP31812PU-N - RBFOX3 / NeuN
AP31812PU-N - RBFOX3 / NeuN
CA1 region of a transgenic Mouse expressing the eGFP gene product under control of the Thy-1 promoter. Green is eGFP autofluorescence. Red is Cy-3 secondary antibody labeling Chicken anti-Neu-N (Fox-3) positive neuronal nuclei. Picture courtesy of Dr. Felix Eckenstein, University of Vermont.
TA309831 - RBFOX3 / NeuN
Western Blot: RBFOX/Neun Antibody (1B7) Western blot of crude rat brain extract stained with anti-RBFOX. RBFOX/Neun is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa.
TA309831 - RBFOX3 / NeuN
Immunofluorescence: RBFOX3/NeuN Antibody (1B7) - Rat brain neural cultures stained with anti-RBFOX (red), a chicken polyclonal antibody to GFAP (green) and DNA (blue). Anti-RBFOX reveals strong nuclear and distal cytoplasmic staining for RBFOX/Neun and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This RBFOX/Neun (1B7) antibody is therefore an excellent marker of neuronal cells.
TA309832 - RBFOX3 / NeuN
Immunohistochemistry: RBFOX/Neun Antibody - IHC analysis of RBFOX3/Neun in mouse brain using DAB with hematoxylin counterstain.
TA309832 - RBFOX3 / NeuN
Immunocytochemistry: RBFOX/Neun Antibody - NeuN/RBFOX3 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
TA323300 - RBFOX3 / NeuN
Predicted band size: 34 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
TA323300 - RBFOX3 / NeuN
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using RBFOX3 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification: x200)
TA323301 - RBFOX3 / NeuN
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using RBFOX3 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification: x200)
TA326863 - RBFOX3 / NeuN
Western blot analysis of extracts of various cell lines, using RBFOX3 antibody.

Items 1 to 20 of 22 total

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RBFOX3 / NeuN antibody
  Mouse Monoclonal RBFOX3/NeuN Antibody (1B7)  
Western Blot: RBFOX/Neun Antibody (1B7) Western blot of crude rat brain extract stained with anti-RBFOX. RBFOX/Neun is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa. Mouse IgG2a 1B7 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €416.00
  OriGene Technologies, Inc.
+1 additional image

RBFOX3 / NeuN antibody
  Rabbit Polyclonal RBFOX3/NeuN Antibody  
Immunohistochemistry: RBFOX/Neun Antibody - IHC analysis of RBFOX3/Neun in mouse brain using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF
0.1 ml / €451.00
  OriGene Technologies, Inc.
+1 additional image

RBFOX3 / NeuN antibody
  Anti-RBFOX3 Rabbit Polyclonal Antibody  
Predicted band size: 34 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

RBFOX3 / NeuN antibody
  Anti-RBFOX3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using RBFOX3 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

RBFOX3 / NeuN antibody
  NeuN Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using RBFOX3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

RBFOX3 / NeuN antibody
Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with NeuN antibody Cat.-No. AM10122SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of granular neuronal cells. Mouse IgG1 A60 Supernatant Hu, Ms P
1 ml / €450.00
  Acris Antibodies GmbH

RBFOX3 / NeuN antibody
RBFOX3 / NeuN Chicken Ig Aff - Purified Hu, Ms ICC/IF, P
0.1 mg / €500.00
  Acris Antibodies GmbH
+1 additional image

RBFOX3 / NeuN antibody
FOX3 (red) and and beta-tubulin (Green) staining of cochlear ganglion of a neonatal mouse. Protocol on Data-sheet. Chicken Aff - Purified Ms, Rt C, ICC/IF
0.1 ml / €210.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

RBFOX3 - human gene knockout kit via CRISPR
RBFOX3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RBFOX3 - human gene knockout kit via CRISPR
RBFOX3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RBFOX3 - human gene knockout kit via CRISPR
RBFOX3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RBFOX3 - human gene knockout kit via CRISPR
RBFOX3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rbfox3 - mouse gene knockout kit via CRISPR
Rbfox3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rbfox3 - mouse gene knockout kit via CRISPR
Rbfox3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

RBFOX3 overexpression lysate
RBFOX3 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, D11Bwg0517e (GFP-tagged) - Mouse DNA segment, Chr 11, Brigham & Women's Genetics 0517 expressed (cDNA clone MGC:62856 IMAGE:6494361), 200 uL, >10^7 TU/mL
Lenti ORF particles, D11Bwg0517e (GFP-tagged) - Mouse DNA segment, Chr 11, Brigham & Women's Genetics 0517 expressed (cDNA clone MGC:62856 IMAGE:6494361), 200 uL, >10^7 TU/mL  
200 µl / €1,206.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, D11Bwg0517e (Myc-DDK-tagged) - Mouse DNA segment, Chr 11, Brigham & Women's Genetics 0517 expressed (cDNA clone MGC:62856 IMAGE:6494361), 200 uL, >10^7 TU/mL
Lenti ORF particles, D11Bwg0517e (Myc-DDK-tagged) - Mouse DNA segment, Chr 11, Brigham & Women's Genetics 0517 expressed (cDNA clone MGC:62856 IMAGE:6494361), 200 uL, >10^7 TU/mL  
200 µl / €1,082.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Rbfox3 (GFP-tagged) - Mouse RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Rbfox3 (GFP-tagged) - Mouse RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,020.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Rbfox3 (GFP-tagged) - Mouse RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, Rbfox3 (GFP-tagged) - Mouse RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €907.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Rbfox3 (Myc-DDK-tagged) - Mouse RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Rbfox3 (Myc-DDK-tagged) - Mouse RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €907.00
  OriGene Technologies, Inc.
 

Items 1 to 20 of 22 total

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22 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (6)
  2. Lysate (1)
  3. Mouse Lenti ORF Particle (7)
  4. Primary Antibody (8)
Clonality
  1. Polyclonal (6)
  2. Monoclonal (2)
Target
  1. RBFOX3 / NeuN (8)
Reactivity
  1. Mouse (Ms) (8)
  2. Human (Hu) (7)
  3. Rat (Rt) (5)
  4. Bovine (Bov) (1)
  5. Porcine (Por) (1)
Applications
  1. Immunocytochemistry/​Immunofluorescence (ICC/IF) (4)
  2. Paraffin Sections (P) (4)
  3. Western blot / Immunoblot (WB) (3)
  4. Frozen sections (C) (2)
  5. Enzyme Immunoassay (E) (2)
Presentation
  1. Purified (5)
  2. Aff - Purified (2)
  3. Supernatant (1)
Host
  1. Rabbit (4)
  2. Chicken (2)
  3. Mouse (2)
Images
  1. 1 image (8)
Images: Applications
  1. IHC (3)
  2. Western blot / Immunoblot (WB) (3)
  3. Immunofluorescence (IF) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  5. Paraffin Sections (P) (1)
Species (CRISPR)
  1. Human (4)
  2. Mouse (2)
Donor Vector
  1. Luciferase-Puro (2)
  2. RFP-BSD (2)
  3. GFP-Puro (1)
  4. mBFP-Neo (1)
Manufacturer
  1. Acris - OriGene (21)
  2. Neuromics Antibodies (1)
Service
  • OriGene EU
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  • D-32052 Herford
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  • fax: +49-5221-34606-11
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