RBFOX3 / NeuN antibody

Principal name

RBFOX3 / NeuN antibody

Alternative names for RBFOX3 / NeuN antibody

Fox-1 homolog C, FOX3, RNA binding protein fox-1 homolog 3

SwissProt ID

A6NFN3 (Human), Q0VD23 (Bovin), Q8BIF2 (Mouse)

Gene ID

146713 (RBFOX3), 52897 (Rbfox3)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Ft (Ferret), Salamander, Chk (Chicken), Por (Porcine)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of RBFOX3 / NeuN antibody

NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples.
Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro.
Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

General readings

Mullen RJ et al. NeuN, a neuronal specific nuclear protein in vertebrates. Development. 116(1):201-11 (2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RBFOX3 / NeuN antibody

Western Blot: RBFOX/Neun Antibody (1B7) Western blot of crude rat brain extract stained with anti-RBFOX. RBFOX/Neun is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa. Mouse IgG2a 1B7 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RBFOX3 / NeuN antibody

Immunohistochemistry: RBFOX/Neun Antibody - IHC analysis of RBFOX3/Neun in mouse brain using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

RBFOX3 / NeuN antibody

Predicted band size: 34 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RBFOX3 / NeuN antibody

Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using RBFOX3 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

RBFOX3 / NeuN antibody

Western blot analysis of extracts of various cell lines, using RBFOX3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RBFOX3 / NeuN antibody

Formalin-Fixed, Paraffin-Embedded Human cerebellum stained with NeuN antibody Cat.-No. AM10122SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of granular neuronal cells. Mouse IgG1 A60 Supernatant Hu, Ms P
1 ml / €410.00
  Acris Antibodies GmbH

RBFOX3 / NeuN antibody

RBFOX3 / NeuN Chicken Ig Aff - Purified Hu, Ms ICC/IF, P
0.1 mg / €440.00
  Acris Antibodies GmbH

RBFOX3 / NeuN antibody

FOX3 (red) and and beta-tubulin (Green) staining of cochlear ganglion of a neonatal mouse. Protocol on Data-sheet. Chicken Aff - Purified Ms, Rt C, ICC/IF
0.1 ml / €180.00
  Neuromics Antibodies

RBFOX3 / NeuN antibody

RBFOX3 / NeuN Rabbit Aff - Purified Bov, Hu, Ms ICC/IF, P
25 µl / €200.00
  Novus Biologicals Inc.

RBFOX3 / NeuN antibody

Immunohistochemistry-Paraffin: RBFOX/Neun Antibody [NBP1-89821] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

RBFOX3 overexpression lysate

RBFOX3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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