RBL2 / p130 antibody

Principal name

RBL2 / p130 antibody

Alternative names for RBL2 / p130 antibody

Retinoblastoma-like protein 2, RB2, 130 kDa retinoblastoma-associated protein, p130, Retinoblastoma-related protein 2, RBR-2, pRb2

SwissProt ID

O55081 (Rat), Q08999 (Human), Q64700 (Mouse)

Gene ID

5934 (RBL2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Dky (Donkey), Mky (Monkey), Chimp (Chimpanzee), Bov (Bovine), Can (Canine), Eq (Equine), Op (Opossum), Orang-Utan, GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of RBL2 / p130 antibody

RBL2(Retinoblastoma-like protein 2) is a protein that in humans is encoded by the RBL2 gene. It is also known as Retionblastoma-related gene (RB2), P130. Yeung et al. (1993) mapped the RBL2 gene to human chromosome 16q12.2 and rat chromosome 19, using fluorescence in situ hybridization and somatic hybrid cell analysis, respectively. Based on known syntenic relationships among human, rat and mouse, the data suggested that the mouse homolog resides on chromosome 8. Deletions of chromosome 16q have been found in several human neoplasms, including breast, ovarian, hepatic, and prostate cancers, which supports the involvement of RB2 in human cancer as a tumor suppressor gene. Kong et al. (2006)found that RBL2 and RINT1 were essential for telomere length control in human fibroblasts, with loss of either protein leading to longer telomeres. Williams et al. (2006)found that mouse fibroblasts lacking Rb were less susceptible to an oncogenic HRAS allele than wildtype cells. In contrast, p107 (RBL1;) -/- and p130 -/- fibroblasts were more susceptible to HRAS-mediated transformation than wildtype cells.

General readings

1. Bjerregaard, B., et al., Biol. Reprod. 70, 925-935 (2004)
2. Hansen, K., et al., Electrophoresis 20, 372-381 (1999)
Clone DCS-211 is used in these references.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RBL2 / p130 antibody

IHC staining of mouse tissue using anti-pRb2/p130 antiserum. Slides were incubated at 20° C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min.  Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C.  Diaminobenzidine was used as the final chromogen. Rabbit Serum Hu, Ms, Rt C, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RBL2 / p130 (SPA310) antibody

WB using Anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4?. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4?. ECL was used for detection. Rabbit IgG Aff - Purified Bov, Can, Chimp, Eq, Hu, Op, Orang-Utan, Rt E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RBL2 / p130 antibody

Host: Rabbit; Target Name: RBL2; Sample Tissue: Liver Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RBL2 / p130 antibody

Figure 1. Western Blot analysis of AM05251PU-N antibody. Mouse IgG1 KAB40 Purified Hu, Ms, Rt WB
0.1 mg / €360.00
  Acris Antibodies GmbH

RBL2 / p130 antibody

RBL2 / p130 Mouse IgG1 DCS211.6 Purified Hu, Ms WB
0.1 mg / €340.00
  Acris Antibodies GmbH

RBL2 / p130 antibody

Figure 1. Immunoprecipitation of RB2 from Jurkat cells with normal Mouse IgG (Lane 1) or AM20033AF-N (Lane 2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with RB2 antibody (AM20033AF-N). Mouse IgG1 DCS-211 Azide Free Hu ICC/IF, IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RBL2 / p130 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, Cerebellum. Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

RBL2 / p130 (SPA310) antibody

WB using Anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4?. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4?. ECL was used for detection. Rabbit IgG Aff - Purified Bov, Can, Chimp, Eq, Hu, Op, Orang-Utan, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

RBL2 / p130 (N-term) antibody

Western blot analysis of RBL2 / p130 Antibody (N-term) Cat.-No AP53602PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the RBL2 antibody detected the RBL2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RBL2 / p130 antibody

RBL2 / p130 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RBL2 / p130 antibody

RBL2 / p130 Mouse IgG2a 4D7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

RBL2 / p130 antibody

RBL2 / p130 Mouse IgG2a 2H7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

RBL2 / p130 antibody

RBL2 / p130 Mouse IgM 1F11 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

RBL2 / p130 antibody

RBL2 monoclonal antibody, clone KAB40 ( Cat # MAB2026 ) recognizes both the phosphorylated and unphosphorylated forms of p130 at aMW of 130kDa. The primary antibody diluted at 1 : 1000. Hu, Mky, Ms
0.1 mg / €400.00
  Abnova Taiwan Corp.

RBL2 / p130 antibody

Immunoblot analysis of cell lysates. Lane 1 : Jurkat T cell lysate and lane 2 : Hela cell lysate. Rabbit Hu WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

RBL2 / p130 antibody

Western blot using RBL2 polyclonal antibody ( Cat # PAB11266 ) shows detection of endogenous RBL2 protein in whole LNCaP cell extracts.
The band at ~130 kDa ( arrowhead ).
The primary antibody was diluted 1 : 500.
Personal communication, Ang Sun, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA. Rabbit Chimp, Hu E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

RBL2 / p130 antibody

Immunofluorescence analysis of HeLa cells, using RBL2 (Ab-952) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

RBL2 / p130 antibody

Immunofluorescence analysis of HeLa cells, using RBL2 (Ab-952) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

RBL2 / p130 antibody

RBL2 / p130 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

RBL2 / p130 antibody

Immunohistochemistry: Rb2 / p130 Antibody (EP2141Y) [NB100-80045] - Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Rb2/p130 Rabbit IgG EP2141Y Hu F, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

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