RBL2 / p130 antibody

Principal name

RBL2 / p130 antibody

Alternative names for RBL2 / p130 antibody

Retinoblastoma-like protein 2, RB2, 130 kDa retinoblastoma-associated protein, p130, Retinoblastoma-related protein 2, RBR-2, pRb2

SwissProt ID

O55081 (Rat), Q08999 (Human), Q64700 (Mouse)

Gene ID

5934 (RBL2), 19651 (Rbl2), 81758

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Chimp (Chimpanzee), Bov (Bovine), Can (Canine), Eq (Equine), Op (Opossum), Orang-Utan, GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of RBL2 / p130 antibody

RBL2(Retinoblastoma-like protein 2) is a protein that in humans is encoded by the RBL2 gene. It is also known as Retionblastoma-related gene (RB2), P130. Yeung et al. (1993) mapped the RBL2 gene to human chromosome 16q12.2 and rat chromosome 19, using fluorescence in situ hybridization and somatic hybrid cell analysis, respectively. Based on known syntenic relationships among human, rat and mouse, the data suggested that the mouse homolog resides on chromosome 8. Deletions of chromosome 16q have been found in several human neoplasms, including breast, ovarian, hepatic, and prostate cancers, which supports the involvement of RB2 in human cancer as a tumor suppressor gene. Kong et al. (2006)found that RBL2 and RINT1 were essential for telomere length control in human fibroblasts, with loss of either protein leading to longer telomeres. Williams et al. (2006)found that mouse fibroblasts lacking Rb were less susceptible to an oncogenic HRAS allele than wildtype cells. In contrast, p107 (RBL1;) -/- and p130 -/- fibroblasts were more susceptible to HRAS-mediated transformation than wildtype cells.

General readings

1. Bjerregaard, B., et al., Biol. Reprod. 70, 925-935 (2004)
2. Hansen, K., et al., Electrophoresis 20, 372-381 (1999)
Clone DCS-211 is used in these references.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RBL2 / p130 antibody

  Rabbit polyclonal anti-p130 Rb2 antibody  
IHC staining of mouse tissue using anti-pRb2/p130 antiserum. Slides were incubated at 20° C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min.  Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C.  Diaminobenzidine was used as the final chromogen. Rabbit Serum Hu, Ms, Rt C, P, WB
0.1 ml / €356.00
  OriGene Technologies, Inc.

RBL2 / p130 (SPA310) antibody

  Rabbit polyclonal pRb2 p130 antibody  
WB using Anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4?. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4?. ECL was used for detection. Rabbit IgG Aff - Purified Bov, Can, Chimp, Eq, Hu, Op, Orang-Utan, Rt E, IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

RBL2 / p130 antibody

  Rabbit Polyclonal anti-RBL2 Antibody  
Host: Rabbit; Target Name: RBL2; Sample Tissue: Hela Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RBL2 / p130 antibody

  Rabbit Polyclonal Anti-RBL2 Antibody  
Host: Rabbit; Target Name: RBL2; Sample Tissue: Liver Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RBL2 / p130 antibody

Figure 1. Western Blot analysis of AM05251PU-N antibody. Mouse IgG1 KAB40 Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

RBL2 / p130 antibody

Figure 1. Immunoprecipitation of RB2 from Jurkat cells with normal Mouse IgG (Lane 1) or AM20033AF-N (Lane 2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with RB2 antibody (AM20033AF-N). Mouse IgG1 DCS-211 Azide Free Hu ICC/IF, IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

RBL2 / p130 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, Cerebellum. Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

RBL2 / p130 (N-term) antibody

Western blot analysis of RBL2 / p130 Antibody (N-term) Cat.-No AP53602PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the RBL2 antibody detected the RBL2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RBL2 / p130 antibody

Immunofluorescence analysis of HeLa cells, using RBL2 (Ab-952) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €230.00
  Assay Biotech

RBL2 / p130 antibody

Immunofluorescence analysis of HeLa cells, using RBL2 (Ab-952) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

RBL2 - human gene knockout kit via CRISPR

RBL2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RBL2 - human gene knockout kit via CRISPR

RBL2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rbl2 - mouse gene knockout kit via CRISPR

Rbl2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rbl2 - mouse gene knockout kit via CRISPR

Rbl2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rbl2 - mouse gene knockout kit via CRISPR

Rbl2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rbl2 - mouse gene knockout kit via CRISPR

Rbl2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RBL2 / p130

  Purified recombinant protein of Homo sapiens retinoblastoma-like 2 (p130) (RBL2)  
RBL2 / p130 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

RBL2 overexpression lysate

RBL2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rbl2 (GFP-tagged) - Mouse retinoblastoma-like 2 (Rbl2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rbl2 (GFP-tagged) - Mouse retinoblastoma-like 2 (Rbl2), 200 uL, >10^7 TU/mL  
200 µl / €1,950.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rbl2 (Myc-DDK-tagged) - Mouse retinoblastoma-like 2 (Rbl2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rbl2 (Myc-DDK-tagged) - Mouse retinoblastoma-like 2 (Rbl2), 200 uL, >10^7 TU/mL  
200 µl / €1,760.00
  OriGene Technologies, Inc.

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