Recoverin antibody

Principal name

Recoverin antibody

Alternative names for Recoverin antibody

RCVRN, RCV1, Protein CAR, Cancer-associated retinopathy protein

SwissProt ID

P21457 (Bovin), P34057 (Mouse), P35243 (Human), Q8MIH6 (Canfa), Q8VH47 (Rat)

Gene ID

5957 (RCVRN), 140936

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Rt (Rat), Ms (Mouse), Ze (Zebrafish), African clawed frog, Sh (Sheep), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Radioimmunoassay (R), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of Recoverin antibody

Light triggers the phototransduction cascade by activating the visual pigment rhodopsin. Phosphorylation of Rho by rhodopsin kinase is required for the recovery of sensitivity after intense illumination. Ca2+ ions act through Ca2+- binding proteins and are implicated in the desensitization of phototransduction. Recoverin is implicated in the regulation of rhodopsin kinase activity that contributes to the adaptation to background illumination in retinal photoreceptor cells. Recoverin, a Ca2+-binding photoreceptor protein, is recognized as an autoantigen of cancer-associated retinopathy (CAR), which is a rare paraneoplastic neurological syndrome characterized by the degeneration of retinal photoreceptors and associated with small-cell lung cancer. Recoverin is a heterogeneously myristoylated protein that inhibits rhodopsin kinase by inhibiting its phosphorylation. Ca2+ is required for Recoverin to bind rhodopsin kinase. In addition, the binding of Recoverin-rhodopsin kinase is weakened by autophosphorylation of the kinase and is strengthened by the presence of ADP. Upon accommodating two Ca2+ ions, Recoverin extrudes a myristoyl group and associates with the lipid bilayer membrane.

General readings

Miyagawa,Y., et al., (2003) Biochem. Biophys. Res. Commun. 300 (3), 669-673

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Recoverin antibody

Western Blot: Recoverin Antibody  -Western blot analysis of Recoverin expression in human brain using anti-RCVRN. Mouse IgG2a 6A55CD6 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Recoverin antibody

Western blot analysis of extracts from NIH-3T3 cells, using Recoverin antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Recoverin antibody

WB Suggested Anti-RCVRN Antibody; Titration: 1.0 ug/ml; Positive Control: Jurkat Whole Cell Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Recoverin antibody

WB Suggested Anti-RCVRN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Recoverin antibody

WB Suggested Anti-RCV1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Recoverin antibody

Western blot (WB) analysis of Recoverin Antibody (Cat.-No.: AP06684PU-N) in extracts from NIH-3T3 cells. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Recoverin (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Recoverin antibody

Recoverin Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Recoverin antibody

Western Blot analysis of RCVRN expression in transfected 293T cell line (H00005957-T01) by RCVRN MaxPab polyclonal antibody.

Lane 1: RCV1 transfected lysate(22 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Western Blot analysis of RCVRN expression in transfected 293T cell line (H00005957-T01) by RCVRN MaxPab polyclonal antibody.

Lane 1: RCV1 transfected lysate(22 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Immunoprecipitation of RCVRN transfected lysate using anti-RCVRN MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RCVRN MaxPab mouse polyclonal antibody (B01) (H00005957-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Recoverin antibody

Western Blot analysis of RCVRN expression in transfected 293T cell line (H00005957-T01) by RCVRN MaxPab polyclonal antibody.

Lane 1: RCVRN transfected lysate(23.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Recoverin antibody

Western Blot analysis of RCV1 expression in transfected 293T cell line by RCV1 monoclonal antibody (M01), clone 4C6.

Lane 1: RCV1 transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4C6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Detection limit for recombinant GST tagged RCV1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 3G10 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Recoverin Mouse IgG1 3G10 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Western Blot analysis of RCV1 expression in transfected 293T cell line by RCV1 monoclonal antibody (M03), clone 1D3.

Lane 1: RCV1 transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 1D3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Recoverin Mouse IgG1 1D3 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Detection limit for recombinant GST tagged RCV1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 5A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Western Blot analysis of RCV1 expression in transfected 293T cell line by RCV1 monoclonal antibody (M05), clone 2H4.

Lane 1: RCV1 transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 2H4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Recoverin antibody

Western Blot analysis of RCV1 expression in transfected 293T cell line by RCV1 monoclonal antibody (M06), clone 4D9.

Lane 1: RCV1 transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4D9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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