RELA / NF-kB p65 antibody

Principal name

RELA / NF-kB p65 antibody

Alternative names for RELA / NF-kB p65 antibody

NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

SwissProt ID

P98152 (Chick), Q04206 (Human), Q04207 (Mouse), Q04865 (Xenla)

Gene ID

5970 (RELA), 19697 (Rela)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Por (Porcine), Gorilla, Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Chimp (Chimpanzee), Av (Birds), Rb (Rabbit), Prim (Primate), Eq (Equine)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), PLA/Proximity ligation assay (PLA), ChIP assay (ChIP)

Background of RELA / NF-kB p65 antibody

NFkB (Nuclear Factor NF-kappa-B) is a pleiotropic transcription factor that plays a role in many biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. It is found as a homo- or heterodimeric complex containing the Rel-like domain containing proteins NFkB p65 (RELA/p65), RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The heterodimeric NFkB p65/p50 complex is the most abundant one. The dimers bind to kappa-B sites at their target genes, with the affinity of the interaction dependent on the subunit composition of the dimer. Furthermore, different dimers act as transcriptional activators or repressors, with the NFkB p65/p50 and p65-c-Rel complexes acting as activators.
NFkB activity is controlled by several different mechanisms, including post-translational modifications, subcellular localisation and interactions with other coactivators or corepressors. NFkB complexes are held in the cytoplasm in an inactive state by interaction with members of the NFkB inhibitor (IkB) family. Typically, phosphorylation of IkB by IkB kinases (IKKs) in response to different activators leads to degradation of the inhibitor, allowing NFkB to translocate into the nucleus. The inhibitory effect of IkBs is primarily exerted through their interaction with NfKB p65.
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3.
Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.

General readings

Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, and Aggarwal BB (2002) Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol 169-11: 6490-7.
General Reference(s): Baldwin, A.S. (2001) Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kB. J. Clinical Investigation 107: 241-246.
Baldwin, A.S., Jr. (2001) Series introduction: the Clin Cantranscription factor NF-kB and human disease. J. Clinical Investigation 107: 3-6.
References for KBM-5 Cells: Beran, M. et al., (1993) Biological properties and growth in SCID mice of a new myelogenous leukemia cell line (KBM-5) derived from chronic myelogenous leukemia cells in the blastic phase. Cancer Res. 53(15):Can't "last" outside a block at D:websitesrockland-inccgi-bingen.cgi line 83, chunk 71. 3603-10.
Beran, M. et al., (1998) Selective inhibition of cell proliferation and BCR-ABL phosphorylation in acute lymphoblastic leukemia cells expressing Mr 190,000 BCR-ABL protein by a tyrosine kinase inhibitor (CGP-57148). Clin Cancer Res. 4(7):1661-72.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RELA / NF-kB p65 pSer276 antibody

Anti-pS529 shows phospho p65 staining in carcinoma cells. Rabbit Serum Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 pSer529 antibody

TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Data contributed by Aggarwal BB, personal communication. Rabbit Serum Hu E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 antibody

anti NFKB p65 (Rel A) monoclonal antibody TA319553 was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4°C and detected with HRP conjugated Rb-anti Mouse antibody 1:40,000 in MB-070 30 min RT. Mouse 27F9.G4 Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 antibody

Immunohistochemical staining of paraffin-embedded Human melanoma tissue using Rabbit polyclonal anti-RELA antibody at (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min). Rabbit IgG Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RELA / NF-kB p65 antibody

Hum. Adult Placenta; Host:Rabbit. Target Name:RELA. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; RELA antibody - middle region (ARP30371_P050) in Hum. Adult Placenta cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RELA / NF-kB p65 antibody

Western blot analysis of NF-kappaB p65 expression in Hela (A); mouse spleen (B); rat heart (C) whole cell lysates. Mouse Aff - Purified Hu, Ms, Rt ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RELA / NF-kB p65 antibody

Western blot analysis of NF-kappaB p65 expression in Hela (A); mouse spleen (B); rat heart (C) whole cell lysates. Mouse Aff - Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RELA / NF-kB p65 antibody

Western blot analysis of NF-kappaB p65 expression in Hela (A); mouse spleen (B); rat heart (C) whole cell lysates. Mouse Aff - Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

RELA / NF-kB p65 antibody

Immunohistochemistry-Paraffin: NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS276 shows phospho p65 staining in carcinoma cells. Rabbit Purified Hu E, P, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

RELA / NF-kB p65 antibody

Human prostate stained with Anti-NFkappaB p65 antibody Rabbit Purified Hu, Rb, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

RELA / NF-kB p65 antibody

Human prostate stained with Anti-NFkappaB p65 antibody Rabbit Purified Hu, Rb, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

RELA / NF-kB p65 antibody

Human prostate stained with Anti-NFkappaB p65 antibody Rabbit Purified Hu, Rb, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

RELA / NF-kB p65 antibody

Western blot analysis of extracts from NIH-3T3, using NF-kappaB p65 (Ab-311) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 antibody

Western blot analysis of extracts from K562/RAW264.7. cells, using NF-kappaB p65 (Ab-276) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 pSer536 antibody

Western blot using affinity purified anti-p65 (RelA) pS536 antibody shows detection of p65 phosphorylated at S536. The control blot (left lane) contains 100 ng of purified p65-GST fusion protein. The band is seen (right lane) when this protein is phosphorylated at S536 by IKKß. Personal Communication. J. Brady, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 pSer276 antibody

Affinity purified Anti-NFKB p65 (Rel A) pS276 TA319204 lot 24040 was probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 (NLS specific) antibody

Immunochemical's Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells.  Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody.  Rabbit Aff - Purified Hu E, EMSA, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 antibody

Western Blot of Rabbit anti-NFkB p65 (Rel A) antibody. Lane 1: HeLa cell extract. Lane 2: HeLa cell extract. Load: 35 µg per lane. Primary antibody: NFkB p65 Rel A antibody at 1:5000 for 2 H at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT. Block: 5% BLOTTO 2 H at RT. Predicted/Observed size: ~65 kDa, ~65 kDa for NFkb p65 Rel A. Other band(s): None. Rabbit Serum Hu, Ms, Rt E, EMSA, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 antibody

NFKBp65 Antibody (pS536) (Cat. #TA324641) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow). Rabbit IgG Purified Hu, Ms, Por ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

RELA / NF-kB p65 antibody

RELA Antibody (Center) (Cat. #TA324680) western blot analysis in Hela,K562,Raji,Ramos cell line lysates (35ug/lane).This demonstrates the RELA antibody detected the RELA protein (arrow). Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

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