Resistin antibody

Principal name

Resistin antibody

Alternative names for Resistin antibody

RETN, FIZZ3, HXCP1, RSTN, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor

SwissProt ID

Q762I5 (Bovin), Q99P87 (Mouse), Q9HD89 (Human)

Gene ID

57264 (Retn), 56729 (RETN)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat)

Available hosts

Goat, Rabbit, Chicken, Mouse, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP)

Background of Resistin antibody

Resistin, also known as Found In Inflammatory Zone 3 (FIZZ3) or Adipocyte Secreted Factor(ADSF), is a member of a protein family known as the Resistin-like molecules (RELMs). It is perhaps best known for its potential as a link between obesity and the development of insulin resistance . Other members of this family include RELM-./FIZZ1 and RELM-., which are described in rodents but as yet have no identified human counterparts, and RELM-./FIZZ2. The Resistin amino acid (aa) sequence contains a putative N-terminal signal sequence and a motif containing 11 cysteine residues, 10 of which are characteristic of the RELM family. The protein is thought to be secreted as a dimer and potentially exists in higher order molecular structures resulting from interactions between Resistin dimers or other members of the RELM family . A splice variant in the rat, lacking the signal sequence and localized predominantly to the nucleus, has also been described . A large 3' intron is the primary reason that the mouse genomic sequence is 3-fold larger than the corresponding human sequence . Mouse and human Resistin share only 53 percent identity at the aa level and exhibit differences in expression patterns . In mouse, expression appears primarily inadipose tissues . Although some human studies suggest Resistin is expressed by adipose tissues as well, the most significant source appears to be blood mononuclear cells . In humans, Resistin is also reported to be expressed by pre-adipocytes , placenta , pancreatic islets , and primary leukemia cells . A receptor for Resistin has not yet been described.

Resistin acquired initial attention as a potential link between obesity and glucose regulation. Serum levels were shown to increase in diet-induced and genetic forms of obesity in mice (ob/ob and db/db) and decrease in response to insulin sensitizing drugs (TZDs) . In addition, function-blocking Resistin antibodies enhanced insulin actions while treatment with recombinant Resistin caused glucose intolerance and insulin resistance . Resistin knockout mice exhibit decreased fasting blood glucose levels as a result of reduced hepatic output . To establish a physiological role in humans, several studies have examined whether altered circulating Resistin levels are associated with type 2 diabetes, insulin resistance, and/or obesity. Although some demonstrate significant correlations , others report no correlation , suggesting that in humans fundamental questions remain regarding ResistinÂ?s role in these pathophysiological processes . Resistin expression by human mononuclear cells could indicate a potential role in inflammation. In vitro, Resistin expression by these cells is enhanced by treatment with several pro-inflammatory cytokines including IL-1., TNF-., IFN-., or IL-6 . In addition, Resistin has been shown to activate endothelial cells in vitro, leading to the production of adhesion molecules, Endothelin-1, and chemokines .

General readings

1. Stejskal D, Karpisek M . Adipocyte fatty acid binding protein in a Caucasian population: a new marker of metabolic syndrome. Eur J Clin Invest . Sep;36 (9):621-5 (2006)
2. Yagmur E, Trautwein C, Gressner AM, Tacke F . Resistin serum levels are associated with insulin resistance, disease severity, clinical complications, and prognosis in patients with chronic liver diseases. Am J Gastroenterol . Jun;101 (6):1244-52 (2006)
3. Rothwell SE, Richards AM, Pemberton CJ . Resistin worsens cardiac ischaemia-reperfusion injury. Biochem Biophys Res Commun . Oct 13;349(1):400-7 (2006)
4. Kim KH. et al. A Cysteine-rich Adipose Tissue-specific Secretory Factors Inhibits Adipocte Differentiation. The Journal of Biological Chemistry. 276 (14), 11252-11256, (2001) .
5. Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA: . A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001) .
6. Steppan C.M. at al. A Family of Tissue-specific Resistin-like Molecules. Proc. Natl. Acad. Sci. USA. 98 (2), 502-506, (2001) .
7. Way J.M. et al. Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor g Agonists. The Journal of Biological Chemistry. 276 (28), 25651-25653, (2001) .
8. Way JM, Görgün CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver WR Jr., Willson TM, Kliewe r SA, Hotamisligil GS: . Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor ? Agonists. J Biol Chem. 276, 25651-25653, (2001) .
9. Banerjee RR, Lazar MA: . Dimerization of resistin and resistin-like moleules is etermined by a single cystein. J Biol Chem. 276, 25970-25973, (2001) .
10. Banerjee R.R., Lazar M.A. Dimerization of resistin and resistin-like moleules is etermined by a single cystein. The Journal of Biological Chemistry. 276, 25970-25973, (2001) .
11. Maebuchi M, Machidori M, Urade R, Ogawa T, Moriyama T: . Low resistin levels in adipose tissues and serum high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin. Archiv Biochem Biophys. 416, 164-170 (2003) .
12. Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA: . Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002) .
13. Hartman H.B., Lazar M.A. at al. Mechanisms Regulating Adipocyte Expression of Resistin. The Journal of Biological Chemistry. 277 (22), 19754-19761, (2002) .
14. Steppan CM, Lazar MA: . Resistin and obesity-associated insulin resistance. TRENDS Endocrinol Metab. 13, 18-23, (2002) .
15. Steppan C.M., Lazar M.A. Resistin and obesity-associated insulin resistance. TRENDS in Endocrinology and Metabolism. 13 (1), 18-23, (2002) .
16. Juan CC. at al. Suppressed Gene Expression of Adipocyte Resistin in an Insulin-resistant Rat Model Probably by Elevated Free Fatty Acids. Biochemical and Biophysical Research Communications. 289, 1328-1333, (2001) .
17. Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA: . The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
18. Steppan C.M. et al. The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001) .
19. Pravenec M, Kazdová L, Landa V, Zídek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW: .
Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001) .
20. Fasshauer M. at al. Tumor Necrosis Factor a Is a Negative Regulator of Resistin Gene Expression and Secretion in 3T3-L1 Adipocytes. Biochemical and Biophysical Research Communications. 208, 1027-1031, (2001) .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Resistin antibody

Western Blot: Resistin Antibody at a dilution of 1:500-1:1000. Rabbit IgG Purified Hu C, F, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Resistin antibody

Predicted cell location: Cytoplasm . Positive control: Human gastric and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using RETN antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Resistin antibody

Predicted cell location: Cytoplasm . Positive control: Human ovarian and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using RETN antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Resistin antibody

To detect hResistin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hResistin. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Resistin antibody

To detect hResistin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Resistin (TA328435) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Resistin antibody

To detect mResistin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mResistin. Goat IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Resistin antibody

To detect mResistin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Resistin (TA328554) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mResistin. Goat IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Resistin antibody

Resistin Mouse IgG2b Azide Free Hu E, P, WB
0.5 mg / €220.00
  Acris Antibodies GmbH

Resistin (30-49) antibody

Resistin Goat Purified Hu E
0.1 mg / €500.00
  Acris Antibodies GmbH

Resistin (30-49) antibody

Resistin Goat Purified Hu E
10 µg / €160.00
  Acris Antibodies GmbH

Resistin (30-49) antibody

Resistin Goat Serum Hu E
0.1 ml / €410.00
  Acris Antibodies GmbH

Resistin (30-49) antibody

Resistin Goat Serum Hu E
20 µl / €150.00
  Acris Antibodies GmbH

Resistin (30-49) antibody

Resistin Rabbit Serum Hu E
0.1 ml / €470.00
  Acris Antibodies GmbH

Resistin (30-49) antibody

Resistin Rabbit Serum Hu E
20 µl / €170.00
  Acris Antibodies GmbH

Resistin (33-90) antibody

Resistin Rabbit Serum Hu E
0.1 ml / €410.00
  Acris Antibodies GmbH

Resistin (33-90) antibody

Resistin Rabbit Serum Hu E
20 µl / €150.00
  Acris Antibodies GmbH

Resistin (51-108) antibody

Resistin Rabbit Serum Hu E
0.1 ml / €470.00
  Acris Antibodies GmbH

Resistin (51-108) antibody

Resistin Rabbit Serum Hu E
20 µl / €170.00
  Acris Antibodies GmbH

Resistin (64-88) antibody

Resistin Chicken Purified Hu E
0.1 mg / €450.00
  Acris Antibodies GmbH

Resistin (64-88) antibody

Resistin Chicken Purified Hu E
10 µg / €160.00
  Acris Antibodies GmbH

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