Retinoblastoma-associated protein / RB1 antibody

Principal name

Retinoblastoma-associated protein / RB1 antibody

Alternative names for Retinoblastoma-associated protein / RB1 antibody

Retinoblastoma 1, Rb, p105-Rb, pRb, pp110, OSRC

SwissProt ID

P06400 (Human), P13405 (Mouse), P33568 (Rat), Q90600 (Chick)

Gene ID

5925 (RB1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Mink, Por (Porcine), Mky (Monkey)

Available hosts

Rabbit, Rat, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Dot blot (Dot), ELISA (detection) (E(detection))

Background of Retinoblastoma-associated protein / RB1 antibody

Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene. The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1-S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated which may be sustained through mitosis by differential phosphorylation of state, up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling cell cycle through direct protein-protein interactions, by binding and inactivating the promoters of transcription factors, and through recruitment of histone deacetylase, which deacetylates promoter regions and enhances nucleosome formation, thereby masking transcription enhancing cis elements.

General readings

Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.
Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.
Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.
Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Retinoblastoma-associated protein / RB1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805656) Mouse IgG1 OTI3F11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Retinoblastoma-associated protein / RB1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805671) Mouse IgG1 OTI10H9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Retinoblastoma-associated protein / RB1 pSer807 antibody

Western blot analysis of in extracts from MOLT 4 cell using RB1 (phospho S807) monoclonal antibody, clone 5H12 ( Cat # MAB0005 ). Mouse IgG1 5H12 Hu E, IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

Retinoblastoma-associated protein / RB1 antibody

Colon carcinoma stained with Anti-RB antibody Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Colon carcinoma stained with Anti-RB antibody Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €450.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Colon carcinoma stained with Anti-RB antibody Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using Retinoblastoma (Ab-608) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

  Rabbit polyclonal Retinoblastoma (Ser608) antibody(Phospho-specific)  
Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using Retinoblastoma (Phospho-Ser608) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Predicted band size: 106 kDa . Positive control: K562 cells treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: K562 cells untreated with serum lysate Lane2: K562 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Predicted band size: 106 kDa . Positive control: NIH/3T3 cells treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: NIH/3T3 cells untreated with UV lysate Lane2: NIH/3T3 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of Retinoblastoma expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of Retinoblastoma phosphorylation expression in serum treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of Retinoblastoma phosphorylation expression in serum treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of Retinoblastoma phosphorylation expression in serum treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 (N-term) antibody

MOLT 4 extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Rb antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of Jurkat cell and A431 cell using RB1 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [TA336425] - analysis: HL60 cells were treated with camptothecin for 24 hours. Mouse IgG1 172C1094 Purified Hu, Ms C, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Retinoblastoma-associated protein / RB1 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Retinoblastoma-associated protein / RB1 Mouse IgG1 Rb1 (1F8) Purified Hu ICC/IF, IP, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

  Rabbit Polyclonal anti-Retinoblastoma (Rb) Antibody  
Human Colon Carcinoma stained with anti-Retinoblastoma (Rb) antibody. Rabbit Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

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