Retinoblastoma-associated protein / RB1 antibody

Principal name

Retinoblastoma-associated protein / RB1 antibody

Alternative names for Retinoblastoma-associated protein / RB1 antibody

Retinoblastoma 1, Rb, p105-Rb, pRb, pp110, OSRC

SwissProt ID

P06400 (Human), P13405 (Mouse), P33568 (Rat), Q90600 (Chick)

Gene ID

5925 (RB1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), Chimp (Chimpanzee), Mink, Por (Porcine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Dot blot (Dot), ELISA (detection) (E(detection))

Background of Retinoblastoma-associated protein / RB1 antibody

Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene. The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1-S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated which may be sustained through mitosis by differential phosphorylation of state, up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling cell cycle through direct protein-protein interactions, by binding and inactivating the promoters of transcription factors, and through recruitment of histone deacetylase, which deacetylates promoter regions and enhances nucleosome formation, thereby masking transcription enhancing cis elements.

General readings

1. Aagaard L; Lukas J; Bartkova J; Kjerulff AA; Strauss M; Bartek J. Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines. International Journal of Cancer, 1995, 61(1):115-20.
2. Bartkova J; Lukas J; Muller H; Strauss M; Gusterson B; Bartek J. Abnormal patterns of D-type cyclin expression and G1 regulation in human head and neck cancer. Cancer Research, 1995, 55:949-56.
3. Bartkova J; Lukas J; Muller H; Lutzhoft D; Strauss M; Bartek J. Cyclin D1 protein expression and function in human breast cancer. International Journal of Cancer, 1994, 57(3):353-61.
4. Bartkova J; Lukas J; Strauss M; Bartek J. The PRAD-1/cyclin D1 oncogene product accumulates aberrantly in a subset of colorectal carcinomas. International Journal of Cancer, 1994, 58(4):568-73.
5. Hickman ES; Picksley SM; Vousden KH. Cells expressing HPV16E7 continue cell cycle progression following DNA damage induced p53 activation. Oncogene, 1994, 9:2177-81.
6. Lukas J; Jadayel D; Bartkova J; Nacheva E; Dyer MJ; Strauss M; Bartek J. BCL-1/cyclin D1 oncoprotein oscillates and subverts the G1 phase control in B-cell neoplasms carrying the t(11;14)
translocation. Oncogene, 1994, 9(8):2159-67. 7. Lukas J; Muller H; Bartkova J; Spitkovsky D; Kjerulff AA; Jansen-Durr P; Strauss M; Bartek J. DNA tumor virus oncoproteins and retinoblastoma gene mutations share the ability to relieve the cell's requirement for cyclin D1 function in G1. Journal of Cell Biology, 1994, 125(3):625-38.
8. Bartek J, Vojtesek B, Grand RJA, Gallimore PH, and Lane DP. Cellular localization and T antigen binding of the retinoblastoma protein. Oncogene 7:101-108, 1992.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Retinoblastoma-associated protein / RB1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805656) Mouse IgG1 OTI3F11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Retinoblastoma-associated protein / RB1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805671) Mouse IgG1 OTI10H9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Retinoblastoma-associated protein / RB1 pSer807 antibody

Western blot analysis of in extracts from MOLT 4 cell using RB1 (phospho S807) monoclonal antibody, clone 5H12 ( Cat # MAB0005 ). Mouse IgG1 5H12 Hu E, IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

Retinoblastoma-associated protein / RB1 antibody

Colon carcinoma stained with Anti-RB antibody Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Colon carcinoma stained with Anti-RB antibody Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €440.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Colon carcinoma stained with Anti-RB antibody Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using Retinoblastoma (Ab-608) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of Retinoblastoma expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 (N-term) antibody

MOLT 4 extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Rb antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western blot analysis of Jurkat cell and A431 cell using RB1 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [TA336425] - analysis: HL60 cells were treated with camptothecin for 24 hours. Mouse IgG1 172C1094 Purified Hu, Ms C, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Retinoblastoma-associated protein / RB1 antibody

Human recombinant protein fragment corresponding to amino acids 309-590 of human RB1(NP_000312) produced in E.coli. Mouse IgG2a OTI9E10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Retinoblastoma-associated protein / RB1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805648) Mouse IgG1 OTI1C3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Retinoblastoma-associated protein / RB1 antibody

Human recombinant protein fragment corresponding to amino acids 309-590 of human RB1(NP_000312) produced in E.coli. Mouse IgG1 OTI11C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Retinoblastoma-associated protein / RB1 antibody

Human recombinant protein fragment corresponding to amino acids 309-590 of human RB1(NP_000312) produced in E.coli. Mouse IgG1 OTI1B1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Retinoblastoma-associated protein / RB1 antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with  Retinoblastoma (Rb) Ab (Cat.AM00202) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells. Mouse IgG1 1F8 Supernatant Hu C, P, WB
1 ml / €860.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with  Retinoblastoma (Rb) Ab (Cat.AM00202) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells. Mouse IgG1 1F8 Supernatant Hu C, P
0.1 ml / €250.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Retinoblastoma-associated protein / RB1 Mouse IgG1 XZ104 Purified Hu IP
0.1 mg / €360.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Retinoblastoma-associated protein / RB1 Mouse IgG1 XZ133 Purified Chk, Hu, Ms, Rt IP
0.1 mg / €360.00
  Acris Antibodies GmbH

Retinoblastoma-associated protein / RB1 antibody

Retinoblastoma-associated protein / RB1 Mouse IgG1 C36 Purified Chk, Hu, Ms, Rt IP
0.1 mg / €360.00
  Acris Antibodies GmbH

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