RFC4 / RFC37 antibody
Principal name
RFC4 / RFC37 antibody
Alternative names for RFC4 / RFC37 antibody
Replication factor C subunit 4, Activator 1 subunit 4, RF-C 37 kDa subunit, Activator 1 37 kDa subunit, Replication factor C 37 kDa subunit
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), PLA/Proximity ligation assay (PLA), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of RFC4 / RFC37 antibody
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Gupte,R.S., (2005) Cell Cycle 4 (2), 323-329
Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320](GTX70285) antibody at 1:200 dilution.
Western blot analysis of RFC p37 protein expression in (A) HepG2, (B) LNCAP, (C) MCF7), (D) and SKOV3 whole cell lysates using GTX70285.
p37 antibody detects p37 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p37 protein stained by p37 antibody (GTX70287) diluted at 1:500.Blue: Hoechst 33342 staining.
Detection of human p37 protein in HeLa whole cell lysate using GeneTex p37 polyclonal antiserum GTX70287.
Western Blot: RFC4 Antibody [NB100-233] - _x000d_
Western Blot: RFC4 Antibody [NB100-234] - Whole cell extract (50mcg) from MCF7 and 293T or nuclear extract (50 mcg) from HeLa cells. Affinity purified goat anti-RFC4 used at 0.5 mcg/ml (A) and 1 mcg/ml (B). Chemiluminescence detection
Immunoperoxidase of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Western blot analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( Cat # H00005984-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody (M01), clone 1C12 (Cat # H00005984-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
RFC4 monoclonal antibody (M01), clone 1C12 Western Blot analysis of RFC4 expression in K-562 ( Cat # L009V1 ).
Western Blot analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescent analysis of HepG2 cells, using RFC4 antibody 10806-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
LNCaP cells were subjected to SDS PAGE followed by western blot with 10806-1-AP(RFC4 antibody) at dilution of 1:500
Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP(RFC4 antibody) at dilution of 1:500
MCF7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP(RFC4 antibody) at dilution of 1:500
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP( RFC4 Antibody) at dilution of 1:2000
Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP( RFC4 Antibody) at dilution of 1:2000
1X10^6 HepG2 cells were stained with 0.2ug RFC4 antibody (10806-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
RFC4 MaxPab polyclonal antibody. Western Blot analysis of RFC4 expression in human placenta.
Western Blot analysis of RFC4 expression in transfected 293T cell line (H00005984-T01) by RFC4 MaxPab polyclonal antibody.Lane 1: RFC4 transfected lysate(39.93 KDa).Lane 2: Non-transfected lysate.
RFC4 MaxPab polyclonal antibody. Western Blot analysis of RFC4 expression in HeLa.
Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.
RFC4 MaxPab polyclonal antibody. Western Blot analysis of RFC4 expression in human placenta.
Western Blot analysis of RFC4 expression in transfected 293T cell line (H00005984-T01) by RFC4 MaxPab polyclonal antibody.Lane 1: RFC4 transfected lysate(39.93 KDa).Lane 2: Non-transfected lysate.
RFC4 MaxPab polyclonal antibody. Western Blot analysis of RFC4 expression in HeLa.
RFC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RFC4 expression in human kidney.
Western Blot analysis of RFC4 expression in transfected 293T cell line (H00005984-T02) by RFC4 MaxPab polyclonal antibody.Lane 1: RFC4 transfected lysate(39.70 KDa).Lane 2: Non-transfected lysate.
RFC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RFC4 expression in HeLa.
Western Blot: RFC4 Lysate [NBL1-15294] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for RFC4
Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4(GTX104052) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4(GTX104052) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 (GTX27900) 10% SDS PAGE GTX104052 diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE GTX104052 diluted at 1:500
Human 721_B; WB Suggested Anti-RFC4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. RFC4 is supported by BioGPS gene expression data to be expressed in 721_B.; RFC4 antibody - middle region (ARP46087_P050) in Human 721_B cells using Western Blot
Immunohistochemistry: RFC4 Antibody [NBP1-33083] - Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using NBP1-33083.
Immunofluorescence: RFC4 Antibody [NBP1-33083] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using NBP1-33083.
Western Blot: RFC4 Antibody [NBP1-33083] - Western blot analysis of RFC4 on H1299 lysates (Lane A), Hela cells (Lane B) and Hep G2 lysates (Lane C) using NBP1-33083.
ELISA: RFC4 Antibody [38240002]
RFC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RFC4 expression in HeLa.
RFC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RFC4 expression in human kidney.
Immunoprecipitation of RFC4 transfected lysate using anti-RFC4 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RFC4 MaxPab mouse polyclonal antibody (B01) (H00005984-B01).
Sample (30 ug of whole cell lysate) A: HeLa nucleus 10% SDS PAGE GTX113538 diluted at 1:500
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
Anti-RFC4 mouse monoclonal antibody (TA506183) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4(RC200426).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4 (RC200426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4 (RC211392, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC4.
ELISA: RFC4 Antibody
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308528 | RFC4 / RFC37 antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA506183 | RFC4 / RFC37 antibody |
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 |
Mouse | IgG1 | OTI1A8 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA590357 | RFC4 / RFC37 antibody |
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 |
Rabbit | IgG | Purified | Hu | E, P, WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| H00005984-M01 | RFC4 / RFC37 antibody |
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![Immunoperoxidase of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00005984-M01-3-5-1-L.jpg) |
Mouse | IgG | 1C12 | Purified | Hu | E, P, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA332718 | RFC4 / RFC37 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA339258 | RFC4 / RFC37 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| H00005984-B01 | RFC4 / RFC37 antibody |
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 |
Mouse | Serum | Hu | E(detection), WB |
50 µl /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| +2 additional images |
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| H00005984-B01P | RFC4 / RFC37 antibody |
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 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| +2 additional images |
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| H00005984-D01 | RFC4 / RFC37 antibody |
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 |
Rabbit | Serum | Hu | E(detection), IP, WB |
0.1 ml /
€380.00
|
||
| Abnova Taiwan Corp. | |||||||
| +2 additional images |
|||||||
| H00005984-D01P | RFC4 / RFC37 antibody |
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 |
Rabbit | Purified | Hu | E(detection), WB |
0.1 mg /
€380.00
|
||
| Abnova Taiwan Corp. | |||||||
| +2 additional images |
|||||||
| H00005984-M02 | RFC4 / RFC37 antibody |
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 |
Mouse | IgG2a | 1B2 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| ARP46087_P050 | RFC4 / RFC37 antibody |
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 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Hu, Ms, Rt | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| 38240002 | RFC4 / RFC37 antibody |
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![ELISA: RFC4 Antibody [38240002]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/RFC4 Antibody-ELISA-38240002-img0001.jpg) |
Rabbit | Hu | E, WB |
50 µg /
€400.00
|
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| Novus Biologicals Inc. | |||||||
| NB100-233 | RFC4 / RFC37 (300-350 ) antibody |
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![Western Blot: RFC4 Antibody [NB100-233] - _x000d_](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/p_3ebbe673900c8.jpg) |
Goat | Aff - Purified | Hu | PLA, WB |
0.1 mg /
€440.00
|
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| Novus Biologicals Inc. | |||||||
| NB100-234 | RFC4 / RFC37 (1-50) antibody |
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![Western Blot: RFC4 Antibody [NB100-234] - Whole cell extract (50mcg) from MCF7 and 293T or nuclear extract (50 mcg) from HeLa cells. Affinity purified goat anti-RFC4 used at 0.5 mcg/ml (A) and 1 mcg/ml (B). Chemiluminescence detection](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/p_3ebbe759900cb.jpg) |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€440.00
|
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| Novus Biologicals Inc. | |||||||
| NB100-403 | RFC4 / RFC37 (exon 2) antibody |
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 |
Rabbit | Purified | Hu | IP, PLA, WB |
0.1 ml /
€440.00
|
||
| Novus Biologicals Inc. | |||||||
| NBP1-33083 | RFC4 / RFC37 antibody |
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![Immunohistochemistry: RFC4 Antibody [NBP1-33083] - Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using NBP1-33083.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/111493_3.jpg) |
Rabbit | IgG | Hu | ICC/IF, P, WB |
0.1 ml /
€420.00
|
||
| Novus Biologicals Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300426 | RFC4 / RFC37 |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
|
| OriGene Technologies, Inc. | |||||
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| TP311392 | RFC4 / RFC37 (transcript variant 2) |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
|
| OriGene Technologies, Inc. | |||||
|
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| H00005984-P01-10 | RFC4 / RFC37 |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
