RFC4 / RFC37 antibody
Principal name
RFC4 / RFC37 antibody
Alternative names for RFC4 / RFC37 antibody
Replication factor C subunit 4, Activator 1 subunit 4, RF-C 37 kDa subunit, Activator 1 37 kDa subunit, Replication factor C 37 kDa subunit
Ncbi ID
9606, NP_002907, NP_002907.1, NM_181573, NM_002916, NP_853551, NP_663455.1, NM_145480
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of RFC4 / RFC37 antibody
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
General readings
Gupte,R.S., (2005) Cell Cycle 4 (2), 323-329
Immunoperoxidase of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Western blot analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( Cat # H00005984-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody (M01), clone 1C12 (Cat # H00005984-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
RFC4 monoclonal antibody (M01), clone 1C12 Western Blot analysis of RFC4 expression in K-562 ( Cat # L009V1 ).
Western Blot analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.
RFC4 MaxPab polyclonal antibody. Western Blot analysis of RFC4 expression in human placenta.
Western Blot analysis of RFC4 expression in transfected 293T cell line (H00005984-T01) by RFC4 MaxPab polyclonal antibody.Lane 1: RFC4 transfected lysate(39.93 KDa).Lane 2: Non-transfected lysate.
RFC4 MaxPab polyclonal antibody. Western Blot analysis of RFC4 expression in HeLa.
Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.
RFC4 MaxPab polyclonal antibody. Western Blot analysis of RFC4 expression in human placenta.
Western Blot analysis of RFC4 expression in transfected 293T cell line (H00005984-T01) by RFC4 MaxPab polyclonal antibody.Lane 1: RFC4 transfected lysate(39.93 KDa).Lane 2: Non-transfected lysate.
RFC4 MaxPab polyclonal antibody. Western Blot analysis of RFC4 expression in HeLa.
RFC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RFC4 expression in human kidney.
Western Blot analysis of RFC4 expression in transfected 293T cell line (H00005984-T02) by RFC4 MaxPab polyclonal antibody.Lane 1: RFC4 transfected lysate(39.70 KDa).Lane 2: Non-transfected lysate.
RFC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RFC4 expression in HeLa.
Human 721_B; WB Suggested Anti-RFC4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. RFC4 is supported by BioGPS gene expression data to be expressed in 721_B.; RFC4 antibody - middle region (ARP46087_P050) in Human 721_B cells using Western Blot
RFC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RFC4 expression in HeLa.
RFC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RFC4 expression in human kidney.
Immunoprecipitation of RFC4 transfected lysate using anti-RFC4 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RFC4 MaxPab mouse polyclonal antibody (B01) (H00005984-B01).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
Anti-RFC4 mouse monoclonal antibody (TA506183) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4(RC200426).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4 (RC200426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFC4 rabbit polyclonal antibody. (TA590357)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4 (RC211392, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC4.
ELISA: RFC4 Antibody
Sample (30 ug of whole cell lysate). A: H1299. B: Hela. C: Hep G2 . 10% SDS PAGE. TA308528 diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: mouse liver. 10% SDS PAGE. TA308528 diluted at 1:500.
Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4(TA308528) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4(TA308528) antibody at 1:200 dilution.
Western blot analysis of extracts of various cell lines, using RFC4 antibody.
Immunofluorescence analysis of U20S cell using RFC4 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-RFC4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysateRFC4 is supported by BioGPS gene expression data to be expressed in 721_B
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308528 | RFC4 / RFC37 antibody |
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| Rabbit Polyclonal antibody to RFC4 (replication factor C (activator 1) 4, 37kDa) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€416.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA506183 | RFC4 / RFC37 antibody |
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| RFC4 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8) | |||||||
 |
Mouse | IgG1 | OTI1A8 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA590357 | RFC4 / RFC37 antibody |
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| Rabbit Polyclonal RFC4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
50 µg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
||||||
| TA332718 | RFC4 / RFC37 antibody |
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| Rabbit anti-RFC4 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA339258 | RFC4 / RFC37 antibody |
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| Rabbit Polyclonal Anti-RFC4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN314691 | Rfc4 - mouse gene knockout kit via CRISPR |
||
 |
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314691BN | Rfc4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314691LP | Rfc4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314691RB | Rfc4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300426 | RFC4 / RFC37 (transcript variant 1) |
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| Recombinant protein of human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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| TP311392 | RFC4 / RFC37 (transcript variant 2) |
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| Recombinant protein of human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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Lysates
| Catalog No. | ||
|---|---|---|
| LY405706 | RFC4 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY419019 | RFC4 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC200426L2V | Lenti ORF particles, RFC4 (mGFP-tagged) - Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC211392L2V | Lenti ORF particles, RFC4 (mGFP-tagged) - Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC211392L1V | Lenti ORF particles, RFC4 (Myc-DDK tagged) - Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 2 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205589L2V | Lenti ORF particles, Rfc4 (GFP-tagged) - Mouse replication factor C (activator 1) 4 (Rfc4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| MR205589L1V | Lenti ORF particles, Rfc4 (Myc-DDK-tagged) - Mouse replication factor C (activator 1) 4 (Rfc4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
