RFC5 / RFC36 antibody

Principal name

RFC5 / RFC36 antibody

Alternative names for RFC5 / RFC36 antibody

Replication factor C subunit 5, Activator 1 subunit 5, RF-C 36 kDa subunit, Activator 1 36 kDa subunit, Replication factor C 36 kDa subunit

SwissProt ID

O94697 (Schpo), P34429 (Caeel), P38251 (Yeast), P40937 (Human), Q9D0F6 (Mouse)

Gene ID

5985 (RFC5), 72151 (Rfc5)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of RFC5 / RFC36 antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

General readings

Ohta,S., (2002) J. Biol. Chem. 277 (43), 40362-40367

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFC5 / RFC36 antibody

  Rabbit Polyclonal Anti-RFC5 Antibody  
WB Suggested Anti-RFC5 Antibody Titration: 1 ug/ml; Positive Control: Jurkat cell lysate.RFC5 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFC5 / RFC36 (Center) antibody

Western blot analysis of anti-RFC5 Antibody (Center R185) (Cat.#AP12462PU-N) in Hela cell line lysates (35ug/lane). RFC5 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

RFC5 - human gene knockout kit via CRISPR

RFC5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RFC5 - human gene knockout kit via CRISPR

RFC5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RFC5 - human gene knockout kit via CRISPR

RFC5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RFC5 - human gene knockout kit via CRISPR

RFC5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rfc5 - mouse gene knockout kit via CRISPR

Rfc5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rfc5 - mouse gene knockout kit via CRISPR

Rfc5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rfc5 - mouse gene knockout kit via CRISPR

Rfc5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rfc5 - mouse gene knockout kit via CRISPR

Rfc5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

RFC5 overexpression lysate

RFC5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RFC5 (mGFP-tagged) - Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFC5 (mGFP-tagged) - Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFC5 (mGFP-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFC5 (mGFP-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFC5 (mGFP-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFC5 (mGFP-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFC5 (mGFP-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFC5 (mGFP-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFC5 (Myc-DDK tagged) - Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, RFC5 (Myc-DDK tagged) - Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFC5 (Myc-DDK-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFC5 (Myc-DDK-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFC5 (Myc-DDK-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFC5 (Myc-DDK-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFC5 (Myc-DDK-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFC5 (Myc-DDK-tagged)-Human replication factor C (activator 1) 5, 36.5kDa (RFC5), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rfc5 (GFP-tagged) - Mouse replication factor C (activator 1) 5 (Rfc5), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rfc5 (GFP-tagged) - Mouse replication factor C (activator 1) 5 (Rfc5), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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