RFC5 / RFC36 antibody

Principal name

RFC5 / RFC36 antibody

Alternative names for RFC5 / RFC36 antibody

Replication factor C subunit 5, Activator 1 subunit 5, RF-C 36 kDa subunit, Activator 1 36 kDa subunit, Replication factor C 36 kDa subunit

SwissProt ID

O94697 (Schpo), P34429 (Caeel), P38251 (Yeast), P40937 (Human), Q9D0F6 (Mouse)

Gene ID

5985 (RFC5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Ye (Yeast)

Available hosts

Goat, Rabbit, Mouse

Available applications

PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of RFC5 / RFC36 antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

General readings

Ohta,S., (2002) J. Biol. Chem. 277 (43), 40362-40367

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFC5 / RFC36 antibody

WB Suggested Anti-RFC5 Antibody Titration: 1 ug/ml; Positive Control: Jurkat cell lysate.RFC5 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFC5 / RFC36 (Center) antibody

Western blot analysis of anti-RFC5 Antibody (Center R185) (Cat.#AP12462PU-N) in Hela cell line lysates (35ug/lane). RFC5 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RFC5 / RFC36 antibody

RFC5 / RFC36 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RFC5 / RFC36 antibody

RFC5 / RFC36 Mouse IgG2a 1B1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RFC5 / RFC36 antibody

Human kidney; RFC5 antibody - N-terminal region (ARP46172_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

RFC5 / RFC36 antibody

Western Blot: RFC5 Antibody [NB100-235] - Goat Aff - Purified Hu PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

RFC5 / RFC36 antibody

Western Blot: RFC5 Antibody [NBP1-58108] - This Anti-RFC5 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Can, Hu P, WB
50 µg / €440.00
  Novus Biologicals Inc.

RFC5 / RFC36 antibody

Immunohistochemistry-Paraffin: RFC5 Antibody [NBP1-87137] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFC5 / RFC36

RFC5 / RFC36 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RFC5 / RFC36

RFC5 / RFC36 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RFC5 overexpression lysate

RFC5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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