RFFL / RNF189 antibody

Principal name

RFFL / RNF189 antibody

Alternative names for RFFL / RNF189 antibody

CARP2, CARP-2, Fring, RNF34L, RIFIFYLIN, E3 ubiquitin-protein ligase rififylin, Caspases-8 and -10-associated RING finger protein 2, FYVE-RING finger protein Sakura, RING finger protein 189, RING finger protein 34-like

SwissProt ID

Q6ZQM0 (Mouse), Q8CIN9 (Rat), Q8WZ73 (Human)

Gene ID

117584 (RFFL)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of RFFL / RNF189 antibody

RFFL, also known as Rififylin, RING finger and FYVE-like domaincontaining protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kappaB activation. RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein.

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFFL / RNF189 antibody

WB using anti-RFFL antibody shows detection of RFFL (arrowhead) in lysate. Lanes correspond to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 µl of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RFFL. Primary antibody was used at a 1:1,000 dilution. Rabbit IgG Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.5 mg / €325.00
  OriGene Technologies, Inc.

RFFL / RNF189 (1-363) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex. Rabbit Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RFFL / RNF189 antibody

RFFL / RNF189 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RFFL / RNF189 antibody

Western blot analysis of RFFL over-expressed 293 cell line, cotransfected with RFFL Validated Chimera RNAi ( Cat # H00117584-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFFL monoclonal antibody (M01), clone 3A4 (Cat # H00117584-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG2a 3A4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RFFL / RNF189 antibody

Immunohistochemistry of RFFL polyclonal antibody ( Cat # PAB11254 ) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden. Rabbit Bov, Hu, Ms, Rt E, P, WB
0.5 mg / €560.00
  Abnova Taiwan Corp.

RFFL / RNF189 antibody

RFFL / RNF189 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RFFL / RNF189 antibody

Western Blot: RFFL Antibody [NBP1-55375] - This Anti-RFFL antibody was used in Western Blot of THP-1       tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

RFFL / RNF189 antibody

Immunohistochemistry-Paraffin: Rffl Antibody [NBP1-83426] Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

RFFL / RNF189 antibody

Immunohistochemistry-Paraffin: Rffl Antibody [NBP1-83427] Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFFL / RNF189

RFFL / RNF189 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RFFL / RNF189

RFFL / RNF189 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RFFL / RNF189

RFFL / RNF189 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RFFL / RNF189

RFFL / RNF189 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

13 Item(s)

per page
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