RFI / RNF34 antibody

Principal name

RFI / RNF34 antibody

Alternative names for RFI / RNF34 antibody

CARP1, CARP-1, RIFF, E3 ubiquitin-protein ligase RNF34, Caspases-8 and -10-associated RING finger protein 1, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein

SwissProt ID

Q5E9J6 (Bovin), Q6AYH3 (Rat), Q969K3 (Human), Q99KR6 (Mouse)

Gene ID

80196 (RNF34), 80751 (Rnf34)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Prim (Primate), Rt (Rat), Chk (Chicken), Bt (Bat), Eq (Equine), Fi (Fish), Hst (Hamster), Mky (Monkey), Op (Opossum), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), Bear, Orang-Utan, GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of RFI / RNF34 antibody

RNF34 contains a RINF finger, a motif known to be involved in protein-protein and protein-D interactions. This protein interacts with DJA3/hTid-1, which is a DJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis.The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-D interactions. This protein interacts with DJA3/hTid-1, which is a DJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Altertively spliced transcript variants encoding distinct isoforms have been reported.

General readings

1. Sasaki S, Nakamura T, Arakawa H, Mori M, Watanabe T, Nagawa H, Croce CM.
Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer.
Oncogene. 2002 Jul 25;21(32):5024-30.
PMID: 12118383

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFI / RNF34 (N-term) antibody

  Goat Anti-RNF34 / RFI (N Terminus) Antibody  
TA305687 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

RFI / RNF34 (1-373) antibody

  Rabbit polyclonal anti-RNF34 antibody  
WB using anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 µl of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Rabbit IgG Purified Bov, Can, Hu, Ms, Orang-Utan, Rt E, WB
0.5 mg / €419.00
  OriGene Technologies, Inc.

RFI / RNF34 antibody

  Rabbit Polyclonal Anti-RNF34 Antibody  
WB Suggested Anti-RNF34 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFI / RNF34 antibody

RFI / RNF34 Rabbit IgG Purified Bov, Can, Hu, Ms, Prim, Rt E, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

RFI / RNF34 (1-373) antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RFI / RNF34 (3-14) antibody

Human Prostate (formalin-fixed, paraffin-embedded) stained with RNF34 antibody AP22488PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bov, Elephant, Eq, Hst, Hu, Ms, Platypus, Rb, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

RFI / RNF34 (C-term) antibody

RNF34 Antibody (C-term) (Cat. #AP53691PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the RNF34 antibody detected the RNF34 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

RNF34 - human gene knockout kit via CRISPR

RNF34 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RNF34 - human gene knockout kit via CRISPR

RNF34 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RNF34 - human gene knockout kit via CRISPR

RNF34 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RNF34 - human gene knockout kit via CRISPR

RNF34 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rnf34 - mouse gene knockout kit via CRISPR

Rnf34 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rnf34 - mouse gene knockout kit via CRISPR

Rnf34 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rnf34 - mouse gene knockout kit via CRISPR

Rnf34 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rnf34 - mouse gene knockout kit via CRISPR

Rnf34 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFI / RNF34 (transcript variant 2)

  Recombinant protein of human ring finger protein 34 (RNF34), transcript variant 2  
RFI / RNF34 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RFI / RNF34 (1-373, His-tag)

RFI / RNF34 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

RFI / RNF34 (1-373, His-tag)

RFI / RNF34 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

RNF34 overexpression lysate

RNF34 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RNF34 (mGFP-tagged) - Human ring finger protein 34 (RNF34), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, RNF34 (mGFP-tagged) - Human ring finger protein 34 (RNF34), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €950.00
  OriGene Technologies, Inc.

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