RFI / RNF34 antibody

Principal name

RFI / RNF34 antibody

Alternative names for RFI / RNF34 antibody

CARP1, CARP-1, RIFF, E3 ubiquitin-protein ligase RNF34, Caspases-8 and -10-associated RING finger protein 1, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein

SwissProt ID

Q5E9J6 (Bovin), Q6AYH3 (Rat), Q969K3 (Human), Q99KR6 (Mouse)

Gene ID

80196 (RNF34)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Mky (Monkey), Ms (Mouse), Rt (Rat), Prim (Primate), Chk (Chicken), Orang-Utan, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of RFI / RNF34 antibody

RNF34 contains a RINF finger, a motif known to be involved in protein-protein and protein-D interactions. This protein interacts with DJA3/hTid-1, which is a DJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis.The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-D interactions. This protein interacts with DJA3/hTid-1, which is a DJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Altertively spliced transcript variants encoding distinct isoforms have been reported.

General readings

1. Sasaki S, Nakamura T, Arakawa H, Mori M, Watanabe T, Nagawa H, Croce CM.
Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer.
Oncogene. 2002 Jul 25;21(32):5024-30.
PMID: 12118383

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFI / RNF34 (N-term) antibody

TA305687 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

RFI / RNF34 (1-373) antibody

WB using anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 µl of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Rabbit IgG Purified Bov, Can, Hu, Ms, Orang-Utan, Rt E, WB
0.5 mg / €325.00
  OriGene Technologies, Inc.

RFI / RNF34 antibody

WB Suggested Anti-RNF34 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFI / RNF34 antibody

RFI / RNF34 Rabbit IgG Purified Bov, Can, Hu, Ms, Prim, Rt E, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

RFI / RNF34 (1-373) antibody

Brain, cortex: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RFI / RNF34 (3-14) antibody

Human Prostate (formalin-fixed, paraffin-embedded) stained with RNF34 antibody AP22488PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen E, P
50 µg / €470.00
  Acris Antibodies GmbH

RFI / RNF34 (C-term) antibody

RNF34 Antibody (C-term) (Cat. #AP53691PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the RNF34 antibody detected the RNF34 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RFI / RNF34 antibody

Immunofluorescence of purified MaxPab antibody to RNF34 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

RFI / RNF34 antibody

RFI / RNF34 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RFI / RNF34 antibody

RNF34 polyclonal antibody ( Cat # PAB6360 ) ( 3.8µg/ml ) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu E, P
0.1 mg / €400.00
  Abnova Taiwan Corp.

RFI / RNF34 antibody

RFI / RNF34 Goat Bov, Hu, Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

RFI / RNF34 antibody

Western blot using RNF34 polyclonal antibody ( Cat # PAB11257 ) shows detection of human RNF34 ( arrowhead ) in lysate.
Lanes corresponding to empty vector 293T cell lysate ( mock, lane 1 ); RNF34 transfected lysate ( lane 2 ) and RFFL transfected lysate ( lane 3 ), are shown using 20 µg of lysate per lane.
Lysates were prepared from equivalent numbers of cells.
The primary antibody diluted to 1:1,000.
Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD. Rabbit Bov, Hu, Ms, Rt E, ICC/IF, WB
0.5 mg / €560.00
  Abnova Taiwan Corp.

RFI / RNF34 antibody

Human Muscle; WB Suggested Anti-RNF34 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; RNF34 antibody - middle region (ARP40158_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RFI / RNF34 antibody

Immunohistochemistry:RNF34 / RFI (N Terminus) Antibody [NB100-1202]-(3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P
0.1 mg / €400.00
  Novus Biologicals Inc.

RFI / RNF34 antibody

Immunocytochemistry: RNF34 Antibody [NBP1-77904] - Immunocytochemistry: RNF34 Antibody [NBP1-77904] Jurkat cells with DR4 antibody at 10 ug/ml. Rabbit Purified Hu E, ICC/IF, P, WB
0.1 mg / €480.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFI / RNF34 (transcript variant 2)

RFI / RNF34 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RFI / RNF34 (1-373, His-tag)

RFI / RNF34 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

RFI / RNF34 (1-373, His-tag)

RFI / RNF34 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

RFI / RNF34

RFI / RNF34 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RNF34 293T Cell Transient Overexpression Lysate(Denatured)

RNF34 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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