RFPL1 / RNF78 antibody
Principal name
RFPL1 / RNF78 antibody
Alternative names for RFPL1 / RNF78 antibody
Ret finger protein-like 1, RFPL1L, RING finger protein 78
SwissProt ID
O75677 (Human)
Gene ID
5988 (RFPL1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of RFPL1 / RNF78 antibody
- Kit Component:
- - KN211141G1, RFPL1 gRNA vector 1 in pCas-Guide vector
- KN211141G2, RFPL1 gRNA vector 2 in pCas-Guide vector
- KN211141D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of RFPL1 expression in transfected 293T cell line (H00005988-T01) by RFPL1 MaxPab polyclonal antibody.Lane 1: RFPL1 transfected lysate(31.68 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RFPL1 expression in transfected 293T cell line (H00005988-T01) by RFPL1 MaxPab polyclonal antibody.Lane 1: RFPL1 transfected lysate(31.68 KDa).Lane 2: Non-transfected lysate.
Anti-RFPL1 mouse monoclonal antibody (TA501492) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFPL1(RC211141).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFPL1 (RC211141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFPL1.
HEK293T cells transfected with either RC211141 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RFPL1 antibody(TA501492), and then analyzed by flow cytometry.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA501492 | RFPL1 / RNF78 antibody |
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| anti-RFPL1 mouse monoclonal antibody, clone OTI1D3 (formerly 1D3) | |||||||
 |
Mouse | IgG2a | OTI1D3 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN211141 | RFPL1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211141BN | RFPL1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211141LP | RFPL1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211141RB | RFPL1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311141 | RFPL1 / RNF78 |
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| Recombinant protein of human ret finger protein-like 1 (RFPL1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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Lysates
| Catalog No. | ||
|---|---|---|
| LY412149 | RFPL1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC211141L2V | Lenti ORF particles, RFPL1 (mGFP-tagged) - Human ret finger protein-like 1 (RFPL1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€989.00
|
|
| OriGene Technologies, Inc. | ||
| RC211141L1V | Lenti ORF particles, RFPL1 (Myc-DDK tagged) - Human ret finger protein-like 1 (RFPL1) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€886.00
|
|
| OriGene Technologies, Inc. | ||
