RFWD2 / RNF200 antibody

Principal name

RFWD2 / RNF200 antibody

Alternative names for RFWD2 / RNF200 antibody

RING finger protein 200, COP1, E3 ubiquitin-protein ligase RFWD2, RING finger and WD repeat domain protein 2, Constitutive photomorphogenesis protein 1 homolog

SwissProt ID

Q8NHY2 (Human), Q9R1A8 (Mouse)

Gene ID

64326 (RFWD2), 26374 (Rfwd2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of RFWD2 / RNF200 antibody

Rfwd2 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It is involved in JUN ubiquitination and degradation and directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Rfwd2 probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.

General readings

Evidence for functional conservation of a mammalian homologue of the light-responsive plant protein COP1." Wang H., Kang D., Deng X.-W., Wei N. Curr. Biol. 9:711-714(1999) [PubMed: 10395541] [Abstract]

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFWD2 / RNF200 antibody

Western blot analysis of extracts from LOVO cells, using RFWD2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RFWD2 / RNF200 antibody

Western blot analysis of extracts from 3T3 cells, using RFWD2 (Ab-387) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RFWD2 / RNF200 (Center) antibody

Mouse Thymus; WB Suggested Anti-Rfwd2 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Thymus; Rfwd2 antibody - middle region (AP46009PU-N) in Mouse Thymus cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  Acris Antibodies GmbH

RFWD2 / RNF200 antibody

RFWD2 / RNF200 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RFWD2 / RNF200 antibody

Immunofluorescence of monoclonal antibody to RFWD2 on NIH/3T3 cell. [antibody concentration 10 ug/ml] Mouse IgG2b 1E4 Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RFWD2 / RNF200 pSer387 antibody

Western blot analysis of extracts from K562 cells treated with UV 15', using RFWD2 (Phospho-Ser387) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

RFWD2 / RNF200 pSer387 antibody

Western blot analysis of extracts from K562 cells treated with UV 15', using RFWD2 (Phospho-Ser387) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

RFWD2 / RNF200 antibody

Western blot analysis of extracts from LOVO cells, using RFWD2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

RFWD2 / RNF200 antibody

Western blot analysis of extracts from LOVO cells, using RFWD2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

RFWD2 / RNF200 (Center) antibody

Mouse Thymus; WB Suggested Anti-Rfwd2 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Thymus; Rfwd2 antibody - middle region (ARP42955_P050) in Mouse Thymus cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

RFWD2 / RNF200 antibody

RFWD2 / RNF200 Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

RFWD2 / RNF200 antibody

RFWD2 / RNF200 Rabbit IgG Aff - Purified Hu, Ms E, WB
50 µg / €290.00
  Novus Biologicals Inc.

RFWD2 / RNF200 antibody

Immunohistochemistry-Paraffin: COP1 Antibody [NBP1-85516] - Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFWD2 / RNF200 (transcript variant 1)

RFWD2 / RNF200 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RFWD2 / RNF200 (transcript variant 2)

RFWD2 / RNF200 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RFWD2 / RNF200

RFWD2 / RNF200 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RFWD2 / RNF200

RFWD2 / RNF200 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RFWD2 overexpression lysate

RFWD2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

RFWD2 overexpression lysate

RFWD2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

19 Item(s)

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