RFX1 antibody

Principal name

RFX1 antibody

Alternative names for RFX1 antibody

Enhancer factor C, EF-C, MHC class II regulatory factor RFX1, Transcription factor RFX1

SwissProt ID

P22670 (Human), P48377 (Mouse), P48743 (Yeast)

Gene ID

5989 (RFX1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), Por (Porcine), Rb (Rabbit), GP (Guinea Pig), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP)

Background of RFX1 antibody

RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX1 is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Wang,K.R., (2007) J. Biol. Chem. 282 (36), 26167-26177

18 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFX1 (C-term) antibody

RFX1 Antibody (C-term) (Cat. #TA324605) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RFX1 antibody detected the RFX1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

RFX1 antibody

WB Suggested Anti-RFX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFX1 antibody

WB Suggested Anti-RFX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysateRFX1 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFX1 antibody

WB Suggested Anti-RFX1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, GP, Hu, Ms, Por, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFX1 antibody

WB Suggested Anti-Rfx1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:2500; Positive Control: Mouse Spleen Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFX1 antibody

Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RFX1 antibody

RFX1 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

RFX1 antibody

RFX1 Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

RFX1 antibody

Human 293T; WB Suggested Anti-RFX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. RFX1 is supported by BioGPS gene expression data to be expressed in HEK293T.; RFX1 antibody - N-terminal region (ARP38199_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

RFX1 antibody

Transfected 293T; WB Suggested Anti-RFX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; RFX1 antibody - middle region (ARP38200_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

RFX1 antibody

Human MCF-7; WB Suggested Anti-RFX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: MCF7 cell lysate; RFX1 antibody - C-terminal region (P100690_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

RFX1 antibody

Western Blot: RFX1 Antibody [NBP1-52652]-Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.   Affinity purified rabbit anti-RFX1 antibody  used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies NBP1-52653 and NBP1-52654, which recognize downstream epitopes. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

RFX1 antibody

Western Blot: RFX1 Antibody [NBP1-52653]-Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-RFX1 antibody used for IP at 6 mcg/mg lysate.  RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies NBP1-52652 and NBP1-52654, which recognize other epitopes. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

RFX1 antibody

Western Blot: RFX1 Antibody [NBP1-52654]-Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Affinity purified rabbit anti-RFX1 antibody  used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies NBP1-52652 and NBP1-52653, which recognize upstream epitopes. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFX1

RFX1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

RFX1

RFX1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RFX1

RFX1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

RFX1 overexpression lysate

RFX1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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