RFX5 antibody

Principal name

RFX5 antibody

Alternative names for RFX5 antibody

Regulatory factor X 5, DNA-binding protein RFX5

SwissProt ID

P48382 (Human)

Gene ID

5993 (RFX5)

Available reactivities

Can (Canine), Hu (Human), Rb (Rabbit), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA)

Background of RFX5 antibody

RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. RFX is a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.

General readings

Nagarajan,U.M., (2004) J. Immunol. 173 (1), 410-419

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFX5 antibody

Staining of Daudi lysate using RFX5 Antibody Cat.-No AP32341PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RFX5 antibody

  Rabbit polyclonal anti-RFX5 antibody  
Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 µg per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none. Rabbit Ig Fraction Hu, Ms E, EMSA, IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

RFX5 antibody

  Rabbit Polyclonal Anti-RFX5 Antibody  
WB Suggested Anti-RFX5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RFX5 antibody

  Rabbit Polyclonal Anti-RFX5 Antibody  
WB Suggested Anti-RFX5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysateRFX5 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFX5 antibody

RFX5 Rabbit Serum Hu E, EMSA, IP
0.1 ml / €450.00
  Acris Antibodies GmbH

RFX5 antibody

RFX5 Rabbit Ig Fraction Hu E, EMSA, IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

RFX5 antibody

RFX5 Rabbit Serum Hu, Ms E, EMSA, IP
0.1 ml / €450.00
  Acris Antibodies GmbH

RFX5 (N-term) antibody

RFX5 Rabbit Serum Hu, Ms E, EMSA, IP
0.1 ml / €450.00
  Acris Antibodies GmbH

RFX5 (N-term) antibody

RFX5 Rabbit Ig Fraction Hu, Ms E, EMSA, IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

RFX5 - human gene knockout kit via CRISPR

RFX5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RFX5 - human gene knockout kit via CRISPR

RFX5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RFX5 - human gene knockout kit via CRISPR

RFX5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RFX5 - human gene knockout kit via CRISPR

RFX5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFX5 (transcript variant 2)

  Recombinant protein of human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2  
RFX5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

RFX5 overexpression lysate

RFX5 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

RFX5 overexpression lysate

RFX5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

RFX5 overexpression lysate

RFX5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RFX5 (mGFP-tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFX5 (mGFP-tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFX5 (mGFP-tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, RFX5 (mGFP-tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, RFX5 (Myc-DDK tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, RFX5 (Myc-DDK tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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