RFX5 antibody

Principal name

RFX5 antibody

Alternative names for RFX5 antibody

Regulatory factor X 5, DNA-binding protein RFX5

SwissProt ID

P48382 (Human)

Gene ID

5993 (RFX5)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rb (Rabbit), Rt (Rat), Bov (Bovine), Por (Porcine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), ELISA (detection) (E(detection))

Background of RFX5 antibody

RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. RFX is a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.

General readings

Nagarajan,U.M., (2004) J. Immunol. 173 (1), 410-419

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFX5 antibody

Staining of Daudi lysate using RFX5 Antibody Cat.-No AP32341PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

RFX5 antibody

Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 µg per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none. Rabbit Ig Fraction Hu, Ms E, EMSA, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RFX5 antibody

WB Suggested Anti-RFX5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RFX5 antibody

WB Suggested Anti-RFX5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysateRFX5 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFX5 antibody

RFX5 Rabbit Serum Hu E, EMSA, IP
0.1 ml / €440.00
  Acris Antibodies GmbH

RFX5 antibody

RFX5 Rabbit Ig Fraction Hu E, EMSA, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

RFX5 antibody

RFX5 Rabbit Serum Hu, Ms E, EMSA, IP
0.1 ml / €440.00
  Acris Antibodies GmbH

RFX5 (N-term) antibody

RFX5 Rabbit Serum Hu, Ms E, EMSA, IP
0.1 ml / €440.00
  Acris Antibodies GmbH

RFX5 (N-term) antibody

RFX5 Rabbit Ig Fraction Hu, Ms E, EMSA, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

RFX5 antibody

RFX5 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RFX5 antibody

Western Blot analysis of RFX5 expression in transfected 293T cell line (H00005993-T01) by RFX5 MaxPab polyclonal antibody.

Lane 1: RFX5 transfected lysate(65.30 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RFX5 antibody

RFX5 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RFX5 antibody

RFX5 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

RFX5 antibody

Western Blot analysis of RFX5 expression in transfected 293T cell line (H00005993-T02) by RFX5 MaxPab polyclonal antibody.

Lane 1: RFX5 transfected lysate(65.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RFX5 antibody

RFX5 Mouse IgG2a 3B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RFX5 antibody

RFX5 polyclonal antibody ( Cat # PAB11300 ) ( amino acids 320-494 ) is shown to detect RFX5 present in Raji B cell nuclear extract lysates.
Detection occurs using a 1 : 2,500 dilution of antibody. Rabbit Hu E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

RFX5 antibody

Transfected 293T; WB Suggested Anti-RFX5 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; RFX5 antibody - N-terminal region (ARP37992_T100) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Can, Hu, Rb WB
0.1 mg / €310.00
  AVIVA Systems Biology

RFX5 antibody

Human 721_B; WB Suggested Anti-RFX5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. RFX5 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; RFX5 antibody - C-terminal region (ARP37993_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

RFX5 antibody

Western Blot: RFX5 Antibody [NB100-195] - HeLa Cell Nuclear Extract Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

RFX5 antibody

RFX5 Rabbit Agarose Hu IP
0.1 mg / €400.00
  Novus Biologicals Inc.

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