RFXAP antibody

Principal name

RFXAP antibody

Alternative names for RFXAP antibody

RFX-associated protein, Regulatory factor X-associated protein, RFX DNA-binding complex 36 kDa subunit

SwissProt ID

O00287 (Human), Q8VCG9 (Mouse)

Gene ID

5994 (RFXAP), 170767 (Rfxap)

Available reactivities

Hu (Human), Ms (Mouse), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ELISA (detection) (E(detection)), ChIP assay (ChIP)

Background of RFXAP antibody

The identification of transacting factors controlling major histocompatibility complex (MHC) class II gene transcription via the proximal enhancer of the promoter has been greatly facilitated by a genetic approach, namely, the analysis of cell lines that are characterized by regulatory defects abolishing transcription of these genes (Mach et al., 1996). Most of these MHC class II regulatory mutants are cell lines derived from patients suffering from MHC class II deficiency, also referred to as the bare lymphocyte syndrome type II, a rare autosomal recessive disorder characterized by the lack of constitutive and inducible MHC class II expression in all cell types and tissues. The genetic lesions responsible for this lack of expression lie not in MHC class II genes themselves, but in transacting regulatory genes required for their transcription (Mach et al., 1996). Durand et al. (1997) noted that at least 3 different complementation groups (groups A, B, and C) have been identified by means of somatic cell fusion experiments. the defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC class II promoters Durand et al. (1997) isolated a novel gene that encodes the 36-kD subunit of RFX. They called this subunit RFX-associated protein (RFXAP) because it is a subunit of the RFX complex and interacts with RFX5, yet it does not contain the characteristic RFX DNA-binding motif.

General readings

Long,A.B., (2006) Mol. Immunol. 43 (5), 395-409

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFXAP antibody

WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Muscle Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody

WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Mouse Kidney Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody

WB Suggested Anti-RFXAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Pancreas Rabbit IgG Purified GP, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody

ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the antibody against RFXAP and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 ug of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Image shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel). Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody

RFXAP Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RFXAP antibody

RFXAP Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RFXAP antibody

RFXAP MaxPab rabbit polyclonal antibody. Western Blot analysis of RFXAP expression in human kidney. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RFXAP antibody

Detection limit for recombinant GST tagged RFXAP is approximately 3ng/ml as a capture antibody. Mouse IgG2a 1B5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

RFXAP antibody

ChIP results obtained with the RFXAP polyclonal antibody ( Cat # PAB0661 ) . ChIP assays were performed using NALM cells ( a cell line derived from human pre-B leukemia ) . Rabbit Hu ChIP
50 µg / €470.00
  Abnova Taiwan Corp.

RFXAP antibody

Human Pancreas; WB Suggested Anti-RFXAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Pancreas; RFXAP antibody - middle region (ARP33226_P050) in Human Pancreas cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RFXAP (Center) antibody

Mouse Muscle; WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Muscle; Rfxap antibody - middle region (ARP37454_P050) in Mouse Muscle cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

RFXAP (N-term) antibody

Mouse Kidney; WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Mouse Kidney; Rfxap antibody - N-terminal region (ARP37455_P050) in Mouse Kidney cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

RFXAP antibody

RFXAP Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

RFXAP antibody

RFXAP Rabbit Agarose Hu IP
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFXAP

RFXAP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RFXAP

RFXAP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RFXAP

RFXAP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RFXAP

RFXAP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RFXAP 293T Cell Transient Overexpression Lysate(Denatured)

RFXAP 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

RFXAP overexpression lysate

RFXAP overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

per page
  • LinkedIn