Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to Acris Antibodies Inc. (North America)
Home » Target » RFXAP antibody

RFXAP antibody

(reset)

Principal name

RFXAP antibody

Alternative names for RFXAP antibody

RFX-associated protein, Regulatory factor X-associated protein, RFX DNA-binding complex 36 kDa subunit

SwissProt ID

O00287 (Human), Q8VCG9 (Mouse)

Gene ID

5994 (RFXAP), 170767 (Rfxap)

Ncbi ID

NP_000529.1, NP_000529, NM_133231, 9606, NM_000538, NP_573494.1

Available reactivities

Hu (Human), Ms (Mouse), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ELISA (detection) (E(detection)), ChIP assay (ChIP)

Background of RFXAP antibody

The identification of transacting factors controlling major histocompatibility complex (MHC) class II gene transcription via the proximal enhancer of the promoter has been greatly facilitated by a genetic approach, namely, the analysis of cell lines that are characterized by regulatory defects abolishing transcription of these genes (Mach et al., 1996). Most of these MHC class II regulatory mutants are cell lines derived from patients suffering from MHC class II deficiency, also referred to as the bare lymphocyte syndrome type II, a rare autosomal recessive disorder characterized by the lack of constitutive and inducible MHC class II expression in all cell types and tissues. The genetic lesions responsible for this lack of expression lie not in MHC class II genes themselves, but in transacting regulatory genes required for their transcription (Mach et al., 1996). Durand et al. (1997) noted that at least 3 different complementation groups (groups A, B, and C) have been identified by means of somatic cell fusion experiments. the defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC class II promoters Durand et al. (1997) isolated a novel gene that encodes the 36-kD subunit of RFX. They called this subunit RFX-associated protein (RFXAP) because it is a subunit of the RFX complex and interacts with RFX5, yet it does not contain the characteristic RFX DNA-binding motif.

General readings

Long,A.B., (2006) Mol. Immunol. 43 (5), 395-409

20188-1-AP - RFXAP
HeLa cells were subjected to SDS PAGE followed by western blot with 20188-1-AP(RFXAP antibody) at dilution of 1:300
20188-1-AP - RFXAP
HeLa cells were subjected to SDS PAGE followed by western blot with 20188-1-AP(RFXAP antibody) at dilution of 1:1000
ARP33226_P050 - RFXAP
Human Pancreas; WB Suggested Anti-RFXAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Pancreas; RFXAP antibody - middle region (ARP33226_P050) in Human Pancreas cells using Western Blot
ARP37454_P050 - RFXAP
Mouse Muscle; WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Muscle; Rfxap antibody - middle region (ARP37454_P050) in Mouse Muscle cells using Western Blot
ARP37455_P050 - RFXAP
Mouse Kidney; WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Mouse Kidney; Rfxap antibody - N-terminal region (ARP37455_P050) in Mouse Kidney cells using Western Blot
H00005994-D01P - RFXAP
RFXAP MaxPab rabbit polyclonal antibody. Western Blot analysis of RFXAP expression in human kidney.
H00005994-D01P - RFXAP
Western Blot analysis of RFXAP expression in transfected 293T cell line (H00005994-T02) by RFXAP MaxPab polyclonal antibody.Lane 1: RFXAP transfected lysate(28.30 KDa).Lane 2: Non-transfected lysate.
H00005994-M01 - RFXAP
Detection limit for recombinant GST tagged RFXAP is approximately 3ng/ml as a capture antibody.
PAB0661 - RFXAP
ChIP results obtained with the RFXAP polyclonal antibody ( Cat # PAB0661 ) . ChIP assays were performed using NALM cells ( a cell line derived from human pre-B leukemia ) .

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFXAP antibody
WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Muscle Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody
WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Mouse Kidney Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody
WB Suggested Anti-RFXAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Pancreas Rabbit IgG Purified GP, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody
ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the antibody against RFXAP and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 ug of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Image shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel). Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody
RFXAP Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RFXAP antibody
RFXAP Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RFXAP antibody
RFXAP MaxPab rabbit polyclonal antibody. Western Blot analysis of RFXAP expression in human kidney. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.
+1 additional image

RFXAP antibody
Detection limit for recombinant GST tagged RFXAP is approximately 3ng/ml as a capture antibody. Mouse IgG2a 1B5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

RFXAP antibody
ChIP results obtained with the RFXAP polyclonal antibody ( Cat # PAB0661 ) . ChIP assays were performed using NALM cells ( a cell line derived from human pre-B leukemia ) . Rabbit Hu ChIP
50 µg / €470.00
  Abnova Taiwan Corp.

RFXAP antibody
Human Pancreas; WB Suggested Anti-RFXAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Pancreas; RFXAP antibody - middle region (ARP33226_P050) in Human Pancreas cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RFXAP (Center) antibody
Mouse Muscle; WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Muscle; Rfxap antibody - middle region (ARP37454_P050) in Mouse Muscle cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

RFXAP (N-term) antibody
Mouse Kidney; WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Mouse Kidney; Rfxap antibody - N-terminal region (ARP37455_P050) in Mouse Kidney cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

RFXAP antibody
RFXAP Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

RFXAP antibody
RFXAP Rabbit Agarose Hu IP
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RFXAP
RFXAP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RFXAP
RFXAP Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RFXAP
RFXAP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RFXAP
RFXAP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RFXAP 293T Cell Transient Overexpression Lysate(Denatured)
RFXAP 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

RFXAP overexpression lysate
RFXAP overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

per page
20 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (1)
  2. Lysate (Overexpressed) (1)
  3. Primary Antibody (14)
  4. Protein | Growth Factor (4)
Clonality
  1. Polyclonal (13)
  2. Monoclonal (1)
Target
  1. RFXAP (18)
Property
  1. Center (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (10)
  2. Mouse (Ms) (4)
  3. Guinea Pig (GP) (1)
Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Enzyme Immunoassay (E) (2)
  3. ELISA (detection) (E(detection)) (2)
  4. Immunoprecipitation (IP) (2)
  5. ChIP assay (ChIP) (1)
Presentation
  1. Purified (9)
  2. Aff - Purified (4)
  3. Agarose (1)
  4. Serum (1)
Host
  1. Rabbit (11)
  2. Mouse (3)
Images
  1. 1 image (10)
Images: Applications
  1. Western blot / Immunoblot (WB) (7)
  2. ChIP assay (ChIP) (1)
  3. ELISA (capture) (E(capture)) (1)
Species (Protein)
  1. Human (4)
Source
  1. in vitro transl. (2)
Manufacturer
  1. Abnova Taiwan Corp. (10)
  2. Acris - OriGene (5)
  3. AVIVA Systems Biology (3)
  4. Novus Biologicals Inc. (2)
Service
  • OriGene EU
  • Acris Antibodies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info@acris-online.de
  • orders: ordering@acris-online.de
 
© Copyright 1998 - 2016 Acris Antibodies GmbH
  • LinkedIn