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RFXAP antibody

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Principal name

RFXAP antibody

Alternative names for RFXAP antibody

RFX-associated protein, Regulatory factor X-associated protein, RFX DNA-binding complex 36 kDa subunit

SwissProt ID

O00287 (Human), Q8VCG9 (Mouse)

Gene ID

5994 (RFXAP), 170767 (Rfxap)

Ncbi ID

9606, NP_000529, NM_133231, NP_000529.1, NM_000538, NP_573494.1

Available reactivities

Hu (Human), Ms (Mouse), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ChIP assay (ChIP), ELISA (detection) (E(detection))

Background of RFXAP antibody

RFXAP is part of the RFX complex that binds to the X-box of MHC II promoters.Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Long,A.B., (2006) Mol. Immunol. 43 (5), 395-409

PAB0661 - RFXAP
ChIP results obtained with the RFXAP polyclonal antibody ( Cat # PAB0661 ) . ChIP assays were performed using NALM cells ( a cell line derived from human pre-B leukemia ) .
H00005994-M01 - RFXAP
Detection limit for recombinant GST tagged RFXAP is approximately 3ng/ml as a capture antibody.
H00005994-D01P - RFXAP
RFXAP MaxPab rabbit polyclonal antibody. Western Blot analysis of RFXAP expression in human kidney.
H00005994-D01P - RFXAP
Western Blot analysis of RFXAP expression in transfected 293T cell line (H00005994-T02) by RFXAP MaxPab polyclonal antibody.Lane 1: RFXAP transfected lysate(28.30 KDa).Lane 2: Non-transfected lysate.
ARP33226_P050 - RFXAP
Human Pancreas; WB Suggested Anti-RFXAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Pancreas; RFXAP antibody - middle region (ARP33226_P050) in Human Pancreas cells using Western Blot
ARP37454_P050 - RFXAP
Mouse Muscle; WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Muscle; Rfxap antibody - middle region (ARP37454_P050) in Mouse Muscle cells using Western Blot
ARP37455_P050 - RFXAP
Mouse Kidney; WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Mouse Kidney; Rfxap antibody - N-terminal region (ARP37455_P050) in Mouse Kidney cells using Western Blot
TA329979 - RFXAP
WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Muscle
TA329980 - RFXAP
WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Mouse Kidney
TA332359 - RFXAP
WB Suggested Anti-RFXAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Pancreas
TA347274 - RFXAP
ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the antibody against RFXAP and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 ug of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Image shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RFXAP antibody
  Rabbit Polyclonal Anti-Rfxap Antibody  
WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Muscle Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody
  Rabbit Polyclonal Anti-Rfxap Antibody  
WB Suggested Anti-Rfxap Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Mouse Kidney Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody
  Rabbit Polyclonal Anti-RFXAP Antibody  
WB Suggested Anti-RFXAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Pancreas Rabbit IgG Purified GP, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RFXAP antibody
  Rabbit Polyclonal RFX-AP Antibody  
ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the antibody against RFXAP and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 ug of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Image shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel). Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

RFXAP - human gene knockout kit via CRISPR
RFXAP - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RFXAP - human gene knockout kit via CRISPR
RFXAP - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RFXAP - human gene knockout kit via CRISPR
RFXAP - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RFXAP - human gene knockout kit via CRISPR
RFXAP - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rfxap - mouse gene knockout kit via CRISPR
Rfxap - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rfxap - mouse gene knockout kit via CRISPR
Rfxap - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rfxap - mouse gene knockout kit via CRISPR
Rfxap - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rfxap - mouse gene knockout kit via CRISPR
Rfxap - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

RFXAP overexpression lysate
RFXAP overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RFXAP (mGFP-tagged)-Human regulatory factor X-associated protein (RFXAP), 200 uL, >10^7 TU/mL
Lenti ORF particles, RFXAP (mGFP-tagged)-Human regulatory factor X-associated protein (RFXAP), 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, RFXAP (Myc-DDK-tagged)-Human regulatory factor X-associated protein (RFXAP), 200 uL, >10^7 TU/mL
Lenti ORF particles, RFXAP (Myc-DDK-tagged)-Human regulatory factor X-associated protein (RFXAP), 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rfxap (GFP-tagged) - Mouse regulatory factor X-associated protein (Rfxap), 200 uL, >10^7 TU/mL
Lenti ORF particles, Rfxap (GFP-tagged) - Mouse regulatory factor X-associated protein (Rfxap), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Rfxap (Myc-DDK-tagged) - Mouse regulatory factor X-associated protein (Rfxap), 200 uL, >10^7 TU/mL
Lenti ORF particles, Rfxap (Myc-DDK-tagged) - Mouse regulatory factor X-associated protein (Rfxap), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.
 

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Available Filters

Product Type
  1. CRISPR (8)
  2. Human Lenti ORF Particle (2)
  3. Lysate (1)
  4. Mouse Lenti ORF Particle (2)
  5. Primary Antibody (4)
Clonality
  1. Polyclonal (4)
Target
  1. RFXAP (4)
Reactivity
  1. Human (Hu) (2)
  2. Mouse (Ms) (2)
  3. Guinea Pig (GP) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
Presentation
  1. Purified (4)
Host
  1. Rabbit (4)
Images
  1. 1 image (4)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
Species (CRISPR)
  1. Human (4)
  2. Mouse (4)
Donor Vector
  1. GFP-Puro (2)
  2. Luciferase-Puro (2)
  3. RFP-BSD (2)
  4. mBFP-Neo (2)
Manufacturer
  1. Acris - OriGene (17)
Service
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