RGS2 antibody
Principal name
RGS2 antibody
Alternative names for RGS2 antibody
G0S8, GIG31, Regulator of G-protein signaling 2, Cell growth-inhibiting gene 31 protein, G0/G1 switch regulatory protein 8
SwissProt ID
O08849 (Mouse), P41220 (Human), P49808 (Caeel), Q0P5H5 (Bovin), Q3S853 (Pig), Q99188 (Yeast), Q9JHX0 (Rat)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of RGS2 antibody
Regulators of G-protein signaling (RGSs) are a protein family containing a conserved 120 amino acid domain (RGS domain), which bind directly to activated G-alpha subunits (Gai and Gaq) and act as GTPase-activating proteins (GAPs).
One of these proteins, RGS2, is expressed in thyroid, heart. thyroid, and monocyte-derived DCs. The function of RGS2 as a regulator of Gq is also dependent on regulatory sequences in its amino-terminal domain that direct its localization to the plasma membrane. Recent studies have shown that RGS2 is critical for blood pressure homeostasis, and has been suggested to serve as an effector of the NO-cGMP pathway that promotes vascular relaxation based on studies of aortic rings in vitro.
General readings
General / background references:Sinnarajah S et al. RGS2 regulates signal transduction in olfactory neurons by attenuating activation of adenylyl cyclase III. Nature 409:1051-5 (2001). PubMed PMID: 11234015
Immunoperoxidase of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
RGS2 monoclonal antibody (M01), clone 4C4 Western Blot analysis of RGS2 expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged RGS2 is approximately 0.1ng/ml as a capture antibody.
Immunohistochemical of paraffin-embedded human pancreas cancer using 10678-1-AP(RGS2 antibody) at dilution of 1:50 (under 10x lens)
Human brain tissue were subjected to SDS PAGE followed by western blot with 10678-1-AP(RGS2 antibody) at dilution of 1:300
Western blot analysis of E coli derived RGS2 fusion protein using GTX14069 at 1/2000
Anti-RGS2 (GTX14069): Analysis of lysate expressing protein regulator of G-protein signaling 2. Primary antibody Anti-RGS2 (GTX14069) at 1:500 dilution. Secondary antibody Peroxidase Conjugated Rabbit Anti-Chicken IgY (IgG) at 1:10,000 dilution. Developed with TMB Substrate. Lanes 1. Protein Ladder 2. Gene specific transient over-expression HEK293T cell lysate 3. Empty vector transfected control HEK 293T cell lysate.
Immunohistochemistry staining of human brain / cortex (Formalin-fixed, Paraffin-embedded tissue after heat-induced antigen retrieval) using RGS2 antibody Cat.-No. AP07203PU-N (concentration 20 ug/ml).
Western blot analysis of RGS2 in human heart lysate using RGS2 antibody Cat.-No. AP07203PU-N at 2 ug/ml.
Western bot analysis of RGS2 in human heart lysate. Using RGS2 polyclonal antibody ( Cat # PAB0231 ) at 2 µg/ml.
Mouse SP2/0; WB Suggested Anti-Rgs2 Antibody Titration: 1.25ug/ml. Positive Control: SP2/0 cell lysate; Rgs2 antibody - middle region (ARP42827_T100) in Mouse SP2/0 cells using Western Blot
Western Blot analysis of RGS2 expression in transfected 293T cell line (H00005997-T02) by RGS2 MaxPab polyclonal antibody.Lane 1: RGS2 transfected lysate(23.21 KDa).Lane 2: Non-transfected lysate.
Western Blot: RGS2 Lysate [NBL1-15327] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for RGS2
Immunofluorescence analysis of methanol-fixed A549, using RGS2(GTX104944) antibody at 1:200 dilution.
RGS2 antibody detects RGS2 protein by Western blot analysis.A. 30 µg Neuro2A whole cell lysate/extract 12 % SDS-PAGERGS2 antibody (GTX104944) dilution: 1:1000
Sample (30μg whole cell lysate)A:H129912% SDS PAGEGTX104944 diluted at 1:1000
Western Blot: RGS2 Antibody [NBP1-32284]-(30µg whole cell lysate) A:H1299 12% SDS PAGE diluted at 1:1000
Immunofluorescence analysis of methanol-fixed A549 using AP31132PU-N RGS2 antibody at 1/200 dilution.
AP31132PU-N RGS2 antibody staining of H1299 (Lane A) whole cell lysate (30 µg) at 1/1000 dilution, 12% SDS PAGE.
AP31132PU-N RGS2 antibody staining of Neuro2A whole cell lysate (30 µg) at 1/1000 dilution, 12% SDS PAGE.
Human Spleen; Spleen; Rgs2 antibody - N-terminal region (ARP42826_P050) in Human Spleen cells using Immunohistochemistry
Mouse Kidney; WB Suggested Anti-Rgs2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Kidney; Rgs2 antibody - N-terminal region (ARP42826_P050) in Mouse Kidney cells using Western Blot
Recombinant human RGS2, 1-211aa, His-tagged
Immunoprecipitation of RGS2 protein from U87-MG whole cell extracts using 5 μg of RGS2 antibody [N1C3-3] (GTX113349).Western blot analysis was performed using RGS2 antibody [N1C3-3] (GTX113349).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE GTX113349 diluted at 1:1000
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-RGS2 Polyclonal Antibody, Unconjugated (bs-0492R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-RGS2 Polyclonal Antibody, Unconjugated (bs-0492R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA321542 | RGS2 antibody |
||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA321543 | RGS2 antibody |
||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP07203PU-N | RGS2 antibody |
||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
50 µg /
€470.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
|||||||
| H00005997-B01P | RGS2 antibody |
||||||
 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
||
| Abnova Taiwan Corp. | |||||||
| H00005997-M01 | RGS2 antibody |
||||||
![Immunoperoxidase of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00005997-M01-3-5-1-L.jpg) |
Mouse | IgG2a | 4C4 | Purified | Hu | E, P, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +2 additional images |
|||||||
| PAB0231 | RGS2 antibody |
||||||
 |
Rabbit | Hu, Ms, Rt | WB |
0.1 mg /
€400.00
|
|||
| Abnova Taiwan Corp. | |||||||
| ARP42826_P050 | RGS2 (N-term) antibody |
||||||
 |
Rabbit | Aff - Purified | Ms | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| +1 additional image |
|||||||
| ARP42827_T100 | RGS2 antibody |
||||||
 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt | WB |
0.1 mg /
€310.00
|
||
| AVIVA Systems Biology | |||||||
| NB300-365 | RGS2 antibody |
||||||
 |
Chicken | Aff - Purified | Hu, Ms, Rt | ICC/IF, WB |
50 µg /
€490.00
|
||
| Novus Biologicals Inc. | |||||||
| NBP1-32284 | RGS2 antibody |
||||||
![Western Blot: RGS2 Antibody [NBP1-32284]-(30µg whole cell lysate) A:H1299 12% SDS PAGE diluted at 1:1000](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/110710_1.jpg) |
Rabbit | IgG | Hu | ICC/IF, WB |
0.1 mg /
€420.00
|
||
| Novus Biologicals Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302781 | RGS2 |
||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR09915PU-N | RGS2 (1-211, His-tag) |
||||
 |
Human | Purified | > 90 % | E. coli |
50 µg /
€750.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
|||||
| AR09915PU-S | RGS2 (1-211, His-tag) |
||||
|
Human | Purified | > 90 % | E. coli |
10 µg /
€300.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
|||||
| H00005997-P01 | RGS2 |
||||
|
Human | Purified |
2 µg /
€300.00
|
||
| Abnova Taiwan Corp. | |||||
| H00005997-P01-10 | RGS2 |
||||
|
Human |
10 µg /
€380.00
|
|||
| Abnova Taiwan Corp. | |||||
| H00005997-Q01-10 | RGS2 |
||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00005997-Q01-25 | RGS2 |
||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
| NBP1-51045-10 | RGS2 |
||||
|
Human | Purified |
10 µg /
€380.00
|
||
| Novus Biologicals Inc. | |||||
| NBP1-51045-50 | RGS2 |
||||
|
Human | Purified |
50 µg /
€870.00
|
||
| Novus Biologicals Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| H00005997-T01 | RGS2 293T Cell Transient Overexpression Lysate(Denatured) |
|
|
0.1 ml /
€270.00
|
|
| Abnova Taiwan Corp. |
