RGS5 antibody
Principal name
RGS5 antibody
Alternative names for RGS5 antibody
Regulator of G-protein signaling 5
SwissProt ID
O08850 (Mouse), O15539 (Human), P49800 (Rat), Q10955 (Caeel), Q3T0T8 (Bovin), Q864Z2 (Pig)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of RGS5 antibody
The regulator of G protein signaling (RGS) proteins are signal transduction molecules that have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans. Multiple genes homologous to SST2 are present in higher eukaryotes. RGS proteins are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators.The regulator of G protein signaling (RGS) proteins are signal transduction molecules that have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans. Multiple genes homologous to SST2 are present in higher eukaryotes. RGS proteins are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-663 AU130764.1 1-663 664-2587 BX537427.1 499-2422 2588-3235 BU187973.1 34-681 3236-3746 AL600981.1 90-600 3747-4143 AA486366.1 76-472 4144-4358 AA974387.1 113-327 c 4359-4664 BX537427.1 4194-4499 4665-5573 AF176919.1 724-1632 5574-5848 BX537427.1 5409-5683
General readings
Wieland T. et al. (2003) Pharmacol. Ther. 97: 95-115.
RGS5 polyclonal antibody (A01), Lot # 060608JCS1 Western Blot analysis of RGS5 expression in K-562 ( Cat # L009V1 ).
Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 polyclonal antibody.
Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 polyclonal antibody.
Detection limit for recombinant GST tagged RGS5 is approximately 1ng/ml as a capture antibody.
Western Blot analysis of RGS5 expression in transfected 293T cell line (H00008490-T01) by RGS5 MaxPab polyclonal antibody.Lane 1: RGS5 transfected lysate(19.91 KDa).Lane 2: Non-transfected lysate.
RGS5 MaxPab rabbit polyclonal antibody. Western Blot analysis of RGS5 expression in human liver.
Western Blot analysis of RGS5 expression in transfected 293T cell line (H00008490-T02) by RGS5 MaxPab polyclonal antibody.Lane 1: RGS5 transfected lysate(20.90 KDa).Lane 2: Non-transfected lysate.
Western Blot: RGS5 Lysate [NBL1-15335] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for RGS5
Human Heart; WB Suggested Anti-RGS5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; RGS5 antibody - middle region (AVARP09015_P050) in Human Heart cells using Western Blot
Hum. Fetal Heart; RGS5 antibody - middle region (AVARP09015_P050) in Hum. Fetal Heart cells using Western Blot
RGS5 MaxPab rabbit polyclonal antibody. Western Blot analysis of RGS5 expression in human liver.
Immunoprecipitation of RGS5 transfected lysate using anti-RGS5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RGS5 purified MaxPab mouse polyclonal antibody (B01P) (H00008490-B01P).
Immunohistochemical of paraffin-embedded human prostate cancer using 11590-1-AP(RGS5 antibody) at dilution of 1:50 (under 10x lens)
Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:500
Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:500
Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:1000
Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:400
MCF7 cells were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:1000
Human heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:400
Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:1000
Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:500
Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:1000
Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:500
Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP(RGS5 antibody) at dilution of 1:800
RGS5, 1-181aa, Human, His-tagged
Western blot analysis of RGS5 Antibody (N-term) Cat.-No AP53652PU-N in CEM cell line lysates (35ug/lane).This demonstrates the RGS5 antibody detected the RGS5 protein (arrow).
Immunohistochemistry-Paraffin: RGS5 Antibody [NBP1-85813] - Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunofluorescence: RGS5 Antibody [NBP1-85813] - Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
Western Blot: RGS5 Antibody [NBP1-85813] Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: A-431 | Lane 5: Liver | Lane 6: Tonsil
10ug of GTX112807-pro RGS5 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-RGS5 Polyclonal Antibody, Unconjugated (bs-2794R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Western blot analysis of RGS5 expression in MCF7 (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Anti-RGS5 mouse monoclonal antibody (TA503073) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RGS5(RC206857).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (RC206857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.
HEK293T cells transfected with either RC206857 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RGS5 antibody(TA503073), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (RC206857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.
HEK293T cells transfected with either RC206857 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RGS5 antibody(TA503074), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503075)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503075)
Figure from citation: Immunofluorescence of RGS5 protein level by using anti-RGS5 antibody in primary neonatal pig GMCs. View Citation
Figure from citation: Immunofluorescence of RGS5 protein level by using anti-RGS5 antibody in human brain pericytes. Dilution: 1:200 View Citation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (RC206857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.
Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody(TA503075),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC206857 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RGS5 antibody(TA503075), and then analyzed by flow cytometry.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503075 | RGS5 antibody |
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Mouse | IgG1 | OTI1C1 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Top Selling product! Published recently! Highly validated antibody!
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| TA313010 | RGS5 antibody |
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Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA330399 | RGS5 antibody |
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Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA337089 | RGS5 antibody |
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![Western Blot: RGS5 Antibody [TA337089] - Western blot analysis of RGS5 in 1) transfected 293T cells and 2) untransfected 293T cells using TA337089 at 1:500.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-24944-1-W.jpg) |
Rabbit | Purified | Hu | WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA503073 | RGS5 antibody |
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 |
Mouse | IgG1 | OTI 3H6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA503074 | RGS5 antibody |
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Mouse | IgG1 | OTI1E3 | Purified | Hu | F, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP53652PU-N | RGS5 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€370.00
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| Acris Antibodies GmbH | |||||||
| H00008490-A01 | RGS5 antibody |
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Mouse | Serum | Hu | E, WB |
50 µl /
€290.00
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| Abnova Taiwan Corp. | |||||||
| H00008490-B01P | RGS5 antibody |
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Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
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| Abnova Taiwan Corp. | |||||||
| H00008490-D01 | RGS5 antibody |
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 |
Rabbit | Serum | Hu, Ms, Rt | E(detection), IP, WB |
0.1 ml /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00008490-D01P | RGS5 antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | E(detection), WB |
0.1 mg /
€380.00
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| H00008490-M01 | RGS5 antibody |
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Mouse | IgG2b | 4E12 | Purified | Hu | E, WB |
0.1 mg /
€370.00
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| Abnova Taiwan Corp. | |||||||
| PAB0639 | RGS5 antibody |
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Rabbit | Chimp, Hu, Mky, Ms, Rt | WB |
0.1 ml /
€400.00
|
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| Abnova Taiwan Corp. | |||||||
| AVARP09015_P050 | RGS5 antibody |
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Rabbit | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | P, WB |
50 µg /
€350.00
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| AVIVA Systems Biology | |||||||
| +1 additional image |
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| CPA2283-30ul | RGS5 antibody |
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Rabbit | Aff - Purified | Hu, Mky, Ms, Rt | WB |
30 µl /
€190.00
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| Cohesion Biosciences Ltd. | |||||||
| CPA2283-100ul | RGS5 antibody |
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Rabbit | Aff - Purified | Hu, Mky, Ms, Rt | WB |
0.1 ml /
€270.00
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| Cohesion Biosciences Ltd. | |||||||
| CPA2283-200ul | RGS5 antibody |
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Rabbit | Aff - Purified | Hu, Mky, Ms, Rt | WB |
0.2 ml /
€440.00
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| Cohesion Biosciences Ltd. | |||||||
| NBP1-85813 | RGS5 antibody |
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![Immunohistochemistry-Paraffin: RGS5 Antibody [NBP1-85813] - Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/RGS5 Antibody-Immunohistochemistry-Paraffin-NBP1-85813-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€500.00
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| Novus Biologicals Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| AR39110PU-L | RGS5 (1-181, His-tag) |
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Human | Purified | > 90 % | E. coli |
0.5 mg /
€750.00
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| Acris Antibodies GmbH | |||||
| +1 additional image |
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| AR39110PU-N | RGS5 (1-181, His-tag) |
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Human | Purified | > 90 % | E. coli |
0.1 mg /
€300.00
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| Acris Antibodies GmbH | |||||
| +1 additional image |
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