RGS5 antibody
Principal name
RGS5 antibody
Alternative names for RGS5 antibody
Regulator of G-protein signaling 5
SwissProt ID
O08850 (Mouse), O15539 (Human), P49800 (Rat), Q10955 (Caeel), Q3T0T8 (Bovin), Q864Z2 (Pig)
Ncbi ID
9606, NP_003608, NP_003608.1, NP_033089.2, NM_009063, NM_019341, NM_003617
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of RGS5 antibody
The regulator of G protein signaling (RGS) proteins are signal transduction molecules that have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans. Multiple genes homologous to SST2 are present in higher eukaryotes. RGS proteins are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators.The regulator of G protein signaling (RGS) proteins are signal transduction molecules that have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans. Multiple genes homologous to SST2 are present in higher eukaryotes. RGS proteins are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-663 AU130764.1 1-663 664-2587 BX537427.1 499-2422 2588-3235 BU187973.1 34-681 3236-3746 AL600981.1 90-600 3747-4143 AA486366.1 76-472 4144-4358 AA974387.1 113-327 c 4359-4664 BX537427.1 4194-4499 4665-5573 AF176919.1 724-1632 5574-5848 BX537427.1 5409-5683
General readings
Wieland T. et al. (2003) Pharmacol. Ther. 97: 95-115.
RGS5 polyclonal antibody (A01), Lot # 060608JCS1 Western Blot analysis of RGS5 expression in K-562 ( Cat # L009V1 ).
Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 polyclonal antibody.
Detection limit for recombinant GST tagged RGS5 is approximately 1ng/ml as a capture antibody.
Western Blot analysis of RGS5 expression in transfected 293T cell line (H00008490-T01) by RGS5 MaxPab polyclonal antibody.Lane 1: RGS5 transfected lysate(19.91 KDa).Lane 2: Non-transfected lysate.
RGS5 MaxPab rabbit polyclonal antibody. Western Blot analysis of RGS5 expression in human liver.
Western Blot analysis of RGS5 expression in transfected 293T cell line (H00008490-T02) by RGS5 MaxPab polyclonal antibody.Lane 1: RGS5 transfected lysate(20.90 KDa).Lane 2: Non-transfected lysate.
Human Heart; WB Suggested Anti-RGS5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; RGS5 antibody - middle region (AVARP09015_P050) in Human Heart cells using Western Blot
Hum. Fetal Heart; RGS5 antibody - middle region (AVARP09015_P050) in Hum. Fetal Heart cells using Western Blot
RGS5 MaxPab rabbit polyclonal antibody. Western Blot analysis of RGS5 expression in human liver.
Immunoprecipitation of RGS5 transfected lysate using anti-RGS5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RGS5 purified MaxPab mouse polyclonal antibody (B01P) (H00008490-B01P).
RGS5, 1-181aa, Human, His-tagged
Western blot analysis of RGS5 Antibody (N-term) Cat.-No AP53652PU-N in CEM cell line lysates (35ug/lane).This demonstrates the RGS5 antibody detected the RGS5 protein (arrow).
Western blot analysis of RGS5 expression in MCF7 (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Anti-RGS5 mouse monoclonal antibody (TA503073) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RGS5(RC206857).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (RC206857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.
HEK293T cells transfected with either RC206857 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RGS5 antibody(TA503073), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (RC206857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.
HEK293T cells transfected with either RC206857 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RGS5 antibody(TA503074), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503075)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503075)
Figure from citation: Immunofluorescence of RGS5 protein level by using anti-RGS5 antibody in primary neonatal pig GMCs. View Citation
Figure from citation: Immunofluorescence of RGS5 protein level by using anti-RGS5 antibody in human brain pericytes. Dilution: 1:200 View Citation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (RC206857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.
Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody(TA503075),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC206857 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RGS5 antibody(TA503075), and then analyzed by flow cytometry.
Western blot analysis of extracts from NIH/3T3 cells, using RGS5 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RGS5 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-RGS5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human heart
Hum. Fetal Heart
Western Blot: RGS5 Antibody [TA337089] - Western blot analysis of RGS5 in 1) transfected 293T cells and 2) untransfected 293T cells using TA337089 at 1:500.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (RGS5 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (RGS5 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503075 | RGS5 antibody |
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| RGS5 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1) | |||||||
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Mouse | IgG1 | OTI1C1 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Top Selling product! Published recently! Highly validated antibody!
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| TA313010 | RGS5 antibody |
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| Rabbit polyclonal anti-RGS5 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA330399 | RGS5 antibody |
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| Rabbit Polyclonal Anti-RGS5 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | WB |
50 µg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA337089 | RGS5 antibody |
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| Rabbit Polyclonal RGS5 Antibody | |||||||
![Western Blot: RGS5 Antibody [TA337089] - Western blot analysis of RGS5 in 1) transfected 293T cells and 2) untransfected 293T cells using TA337089 at 1:500.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-24944-1-W.jpg) |
Rabbit | Purified | Hu | WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA350344 | RGS5 antibody |
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| Rabbit Polyclonal Anti-RGS5 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA503073 | RGS5 antibody |
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| RGS5 mouse monoclonal antibody, clone OTI 3H6 (formerly 3H6) | |||||||
 |
Mouse | IgG1 | OTI 3H6 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA503074 | RGS5 antibody |
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| RGS5 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3) | |||||||
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Mouse | IgG1 | OTI1E3 | Purified | Hu | F, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AP53652PU-N | RGS5 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€370.00
|
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| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN206857 | RGS5 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN206857BN | RGS5 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206857LP | RGS5 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206857RB | RGS5 - human gene knockout kit via CRISPR |
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|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314741 | Rgs5 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN314741BN | Rgs5 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314741LP | Rgs5 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN314741RB | Rgs5 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| AR39110PU-L | RGS5 (1-181, His-tag) |
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Human | Purified | > 90 % | E. coli |
0.5 mg /
€750.00
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| Acris Antibodies GmbH | |||||
| +1 additional image |
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| AR39110PU-N | RGS5 (1-181, His-tag) |
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|
Human | Purified | > 90 % | E. coli |
0.1 mg /
€300.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
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Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC206857L2V | Lenti ORF particles, RGS5 (mGFP-tagged) - Human regulator of G-protein signaling 5 (RGS5), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| RC206857L1V | Lenti ORF particles, RGS5 (Myc-DDK tagged) - Human regulator of G-protein signaling 5 (RGS5), transcript variant 1 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€780.00
|
|
| OriGene Technologies, Inc. | ||
