RHEB antibody

Principal name

RHEB antibody

Alternative names for RHEB antibody

RHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain

SwissProt ID

P25378 (Yeast), Q15382 (Human), Q56JV3 (Bovin), Q62639 (Rat), Q921J2 (Mouse), Q9VND8 (Drome)

Gene ID

6009 (RHEB)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of RHEB antibody

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway (3-5). TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2 (3).

General readings

Tabancay AP Jr., et al. (2003).J Biol Chem. 10;278(41):39921-30.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RHEB antibody

RHEB monoclonal antibody (M01), clone 2C11. Western Blot analysis of RHEB expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 2C11 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RHEB (N-term) antibody

Western blot analysis of Rheb in rat heart cell lysate with Rheb antibody at (A) 2 and (B) 4 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RHEB (Center) antibody

Western blot analysis of Rheb in mouse brain cell lysate with Rheb antibody at (A) 1, (B) 2, and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RHEB antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200 . The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using RHEB antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

RHEB antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using RHEB antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

RHEB antibody

Western blot analysis of brian cell and Hela cell lysate using RHEB antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RHEB antibody

WB Suggested Anti-RHEB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RHEB antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RHEB Control Peptide

RHEB
50 µg / €160.00
  Acris Antibodies GmbH

RHEB Control Peptide

RHEB
50 µg / €160.00
  Acris Antibodies GmbH

RHEB (C-term) antibody

Formalin fixed, paraffin embedded human skeletal muscle stained with RHEB Antibody (C-term) Cat.-No AP53656PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RHEB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Purified Bov, Hu, Ms, Rt P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RHEB antibody

Immunohistochemical staining of mouse brain with Rabbit anti Human Rheb (SP2154P) Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €330.00
  Acris Antibodies GmbH

RHEB antibody

RHEB Rabbit IgG Purified Hu, Ms, Rt WB
50 µg / €270.00
  Acris Antibodies GmbH

RHEB antibody

Western Blot analysis of RHEB expression in transfected 293T cell line (H00006009-T01) by RHEB MaxPab polyclonal antibody.

Lane 1: RHEB transfected lysate(20.24 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RHEB antibody

RHEB Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RHEB antibody

Immunoprecipitation of RHEB transfected lysate using anti-RHEB MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RHEB MaxPab mouse polyclonal antibody (B01) (H00006009-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

RHEB antibody

Western Blot analysis of RHEB expression in transfected 293T cell line (H00006009-T02) by RHEB MaxPab polyclonal antibody.

Lane 1: RHEB transfected lysate(20.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RHEB antibody

Western Blot analysis of RHEB expression in transfected 293T cell line by RHEB monoclonal antibody (M05), clone 1E12.

Lane 1: RHEB transfected lysate(20.5 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 1E12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RHEB antibody

Western blot analysis of RHEB in rat heart tissue lysate with RHEB polyclonal antibody ( Cat # PAB13142 ) at ( A ) 2 and ( B ) 4 µg/ml. Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

RHEB antibody

Immunohistochemistry of RHEB in mouse brain tissue with RHEB polyclonal antibody ( Cat # PAB13143 ) at 2 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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