RhoA antibody

Principal name

RhoA antibody

Alternative names for RhoA antibody

ARH12, ARHA, RHO12, Transforming protein RhoA, Rho cDNA clone 12

SwissProt ID

P24406 (Canfa), P61585 (Bovin), P61586 (Human), P61589 (Rat), Q9QUI0 (Mouse)

Gene ID

387 (RHOA), 4790387 (A2RWH4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Sh (Sheep)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Dot blot (Dot)

Background of RhoA antibody

Regulates a sigl transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent sigl that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis; vector of the plague; and Yersinia pseudotuberculosis; which causes gastrointestil disorders. Stimulates PKN2 kise activity. May be an activator of PLCE1. Activated by ARHGEF2; which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 sigling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane; via the regulation of GSK3B activity. In turn; membrane-bound APC allows the localization of the MACF1 to the cell membrane; which is required for microtubule capture and stabilization.

General readings

Mol Cell Proteomics. 2007 Mar;6(3):413-24. Epub 2006 Dec 27.
The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis.
Zavzavadjian JR, Couture S, Park WS, Whalen J, Lyon S, Lee G, Fung E, Mi Q, Liu J, Wall E, Santat L, Dhandapani K, Kivork C, Driver A, Zhu X, Chang MS, Randhawa B, Gehrig E, Bryan H, Verghese M, Maer A, Saunders B, Ning Y, Subramaniam S, Meyer T, Simon MI, O'Rourke N, Chandy G, Fraser ID.
Alliance for Cellular Signaling, California Institute of Technology, CA 94305, USA.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Rho antibody

AP15760PU antibody staining of MCF7 cell lysate by Western Blot. Rabbit Purified Hu WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Rho antibody

AP15760PU antibody staining of MCF7 cell lysate by Western Blot. Rabbit Purified Hu WB
1 ml / €520.00
  Acris Antibodies GmbH

Rho antibody

AP15760PU antibody staining of MCF7 cell lysate by Western Blot. Rabbit Purified Hu WB
0.1 ml / €190.00
  Acris Antibodies GmbH

Rho (pan) antibody

Immunofluorescent: RhO Antibody [NB100-91270] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) and CDC42: whole serum NB100-91270 at 1: 2000 dilution. DAPI counter stained. Rabbit Serum Hu, Ms, Rt C, ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Rho antibody

Immunohistochemistry: Rho Antibody (Y486) [NB110-57464] - Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Rho (cat. # 110-57464). Rabbit IgG Y486 Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

RhoA antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1B12 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

RhoA antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1C1 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

RhoA antibody

Immunoperoxidase of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human lymphoma tissue.[antibody concentration 5ug/ml] Mouse IgG1 1B12 Purified Hu E, ICC/IF, P, WB

Regular Price: 0.1 mg / €370.00

Special Price: 0.1 mg / €231.00

  Abnova Taiwan Corp.

RhoA antibody

Immunoperoxidase of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5ug/ml] Mouse IgG1 1C1 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RhoA antibody

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni. Rabbit Serum Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

RhoA antibody

Immunofluorescence: Rho Antibody [NBP1-03452] - rat trigeminal at 1:500 dilution using Sheep antibody to Transforming protein RhoA whole serum (NBP1-03452 in red),  Rabbit antibody to extracellular, N-terminal part of Sortilin whole serum (NB100-91287, in green), DAPI counter stained appearing in blue. Sheep Serum Hu, Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

RhoA antibody

Western blot analysis of extracts from HepG2 cells, using RhoA (Ab-188) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RhoA antibody

Predicted band size: 22 kDa . Positive control: NIH/3T3 cell and Mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: NIH/3T3 cell tissue lysate Lane1: Mouse brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RhoA antibody

Predicted band size: 22 kDa . Positive control: K562, 231 and Hela cell, Human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: K562 cell lysate Lane2: 231 cell lysate Lane3: Hela cell lysate Lane4: Human fetal brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RhoA antibody

Predicted band size: 22 kDa . Positive control: K562, 231 and Hela cell, Human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: K562 cell lysate Lane2: 231 cell lysate Lane3: Hela cell lysate Lane4: Human fetal brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RhoA antibody

Western blot analysis of RhoA expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RhoA antibody

WB Suggested Anti-RHOA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RhoA antibody

Immunohistochemistry (IHC) analyzes of Rho A antibody (Cat.-No.: AP06618PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RhoA antibody

RhoA Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RhoA antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between RHOA and DAAM1 HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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