Rhodopsin antibody

Principal name

Rhodopsin antibody

Alternative names for Rhodopsin antibody

RHO, Opsin-2, OPN2

SwissProt ID

O16005 (Sepof), O16017 (Camlu), O16018 (Camsc), O16019 (Orcvi), O16020 (Proml), O18312 (Camhu), O18315 (Camma), O18481 (Orcau), O18485 (Proor), O18486 (Prose), O18766 (Pig), O42268 (Ictpu), O42294 (Abyko), O42300 (Batmu), O42301 (Batni), O42307 (Cotbo), O42327 (Comdy), O42328 (Cotgr), O42330 (Cotin), O42427 (Limbe), O42431 (Limpa), O42451 (Proje), O42452 (Parkn), O42466 (Taubu), O42604 (Zeufa), O62791 (Delde), O62792 (Glome), O62793 (Mesbi), O62794 (Phovi), O62795 (Paggo), O62796 (Trima), O62798 (Turtr), O93441 (Galml), O93459 (Scyca), P02699 (Bovin), P02700 (Sheep), P08100 (Human), P09241 (Entdo), P15409 (Mouse), P22328 (Chick), P22671 (Lamja), P24603 (Lolfo), P28681 (Crigr), P29403 (Xenla), P31355 (Ranpi), P31356 (Todpa), P32308 (Canfa), P32309 (Carau), P35356 (Procl), P35359 (Danre), P35362 (Sphsp), P35403 (Pommi), P41590 (Astfa), P41591 (Anoca), P49912 (Rabit), P51470 (Ranca), P51488 (Cypca), P51489 (Rat), P52202 (Allmi), P56514 (Bufbu), P56515 (Bufma), P56516 (Rante), P79756 (Gamaf), P79798 (Myrbe), P79807 (Myrvi), P79808 (Neoar), P79809 (Neoau), P79812 (Neosa), P79848 (Poere), P79863 (Leuer), P79898 (Sardi), P79901 (Sarmi), P79902 (Sarpu), P79903 (Sarsp), P79911 (Sarti), P79914 (Sarxa), P87369 (Oryla), Q17053 (Apime), Q17094 (Lolsu), Q17292 (Camab), Q17296 (Catbo), Q28886 (Macfa), Q68J47 (Loxaf), Q6W3E1 (Calpd), Q769E8 (Otocr), Q8HY69 (Smicr), Q90245 (Ambti), Q90305 (Corau), Q90373 (Cotke), Q95KU1 (Felca), Q98980 (Petma), Q9DGG4 (Tetng), Q9YGY9 (Zosop), Q9YGZ0 (Sarpi), Q9YGZ1 (Athbo), Q9YGZ2 (Gobni), Q9YGZ3 (Salpv), Q9YGZ4 (Dicla), Q9YGZ5 (Solso), Q9YGZ6 (Lizau), Q9YGZ7 (Lizsa), Q9YGZ8 (Chelb), Q9YGZ9 (Mugce), Q9YH00 (Litmo), Q9YH01 (Mulsu), Q9YH02 (Spaau), Q9YH03 (Sarsl), Q9YH04 (Dipvu), Q9YH05 (Dipan)

Gene ID

6010 (RHO), 509933, 24717 (Rho)

Available reactivities

Bov (Bovine), Fe (Feline), Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), Mam (Mammalia), Can (Canine), Amph (Amphibian), Av (Birds), Fi (Fish), Frog, Vertebrates, Xen (Xenopus), Re (Reptiles), GP (Guinea Pig), Sh (Sheep)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of Rhodopsin antibody

Rhodopsin is the protein in the mammalian reti responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the Rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate sigling on binding a specific ligand, Rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retil. Retil can exist in two isomeric forms, 11-cis and 11-trans retil. In the dark Rhodopsin is associated with 11-cis retil, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the Rhodopsin making it catalytically active. Activated Rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha and a beta gamma subunit. The alpha subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolysescyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of syptic sigling. So light stimulation results in a reduced rate of photoreceptor syptic release. This information is transmitted through neurons of the reti to the visual centers of the brain. Rhodopsin activity is shut off by phosphorylation under the influence of Rhodopsin kise, the activity of which results in binding of visual arrestin, which prevents Rhodopsin from interacting with and activating more transducin molecules. This basic sigling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kise are found in these pathways.

General readings

General / background references:Treisman JE et al. Opsin expression in the rat retina is developmentally regulated by transcriptional activation. Mol Cell Biol 8:1570-9 (1988). PubMed PMID: 2967911
Hicks D & Barnstable CJ Different rhodopsin monoclonal antibodies reveal different binding patterns on developing and adult rat retina. J Histochem Cytochem 35:1317-28 (1987). PubMed PMID: 3655327
Fekete DM & Barnstable CJ The subcellular localization of rat photoreceptor-specific antigens. J Neurocytol 12:785-803 (1983). PubMed PMID: 6358424
Barnstable CJ Monoclonal antibodies which recognize different cell types in the rat retina. Nature 286:231-5 (1980). PubMed PMID: 6157100

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Rhodopsin (C-term) antibody

Western blot analysis of Rhodopsin in human cell lines using a 1:1000 dilution of anti-RHO. Mouse IgG1 1D4 Purified Ve, Vertebrates. C, E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Rhodopsin (C-term) antibody

Western blot analysis of Rhodopsin in human cell lines using a 1:1000 dilution of anti-RHO. Mouse IgG1 1D4 Purified Ve, Vertebrates. C, E, ICC/IF, IP, WB
25 µg / €250.00
  Acris Antibodies GmbH

Rhodopsin antibody

Immunohistochemical staining of mouse retinal section showing specific immunolabeling of the rhodopsin protein in the rod spherules. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA. Mouse IgG1 1D4 Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Rhodopsin (N-term) antibody

Immunofluorescence of Rho using a dilution of Rhodopsin antibody Mouse IgG1 4D2 Purified Amph, Bov, Fi, Frog, Hu, Mam, Ms, Por, Rt, Ze C, E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Rhodopsin antibody

Western blot analysis of extracts from A549 cells, using RHO antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Rhodopsin antibody

Western Blot: Rhodopsin Antibody (A531) [TA337112] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hy Mouse IgG1 A531 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Rhodopsin antibody

Western Blot: Rhodopsin Antibody (B630) [TA337113] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hy Mouse IgG1 B630 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Rhodopsin (2nd extracell. dom.) antibody

Anti-RHO / Rhodopsin antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Elephant, Fe, Hu, Mky, Ms, Por, Rb, Rt, Ze P
50 µg / €379.00
  OriGene Technologies, Inc.

Rhodopsin antibody

WB Suggested Anti-RHO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

Rhodopsin antibody

Figure 1. Immunohistochemical staining of mouse retinal section showing specific 
immunolabeling of the rhodopsin protein in the rod spherules. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA. Mouse IgG1 1D4 Purified Mam C, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Rhodopsin (N-term) antibody

Immunofluorescence of Rho using a dilution of Rhodopsin antibody Mouse IgG1 4D2 Purified Amph, Bov, Fi, Frog, Hu, Mam, Ms, Por, Rt, Ze C, E, ICC/IF, IP, WB
25 µg / €250.00
  Acris Antibodies GmbH

Rhodopsin antibody

Immunohistochemistry (IHC) analyzes of Rhodopsin antibody (Cat.-No.: AP06577PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Rhodopsin antibody

Rhodopsin Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

Rhodopsin antibody

Rhodopsin Mouse IgG1 RET-P1 Purified Av, Mam, Rt P, WB
0.125 mg / €470.00
  Abnova Taiwan Corp.

Rhodopsin pSer188 antibody

Rhodopsin Rabbit Hu, Ms, Rt C, E, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Rhodopsin antibody

The cell lysate derived from MCF-7 was immunoprobed by RHO polyclonal antibody ( Cat # PAB12727 ) at 1 : 500. An immunoreactive band is observed around ~24 kDa ( 2 ). This band is abolished by pre-incubation with immunizing peptide ( 1 ). Rabbit Hu, Ms, Rt C, E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Rhodopsin pSer334 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Rhodopsin (Phospho-Ser334) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

Rhodopsin pSer334 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Rhodopsin (Phospho-Ser334) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

Rhodopsin antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Rhodopsin (Ab-334) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

Rhodopsin antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Rhodopsin (Ab-334) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

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