RHOT1 antibody
Principal name
RHOT1 antibody
Alternative names for RHOT1 antibody
MIRO-1, ARHT1, Mitochondrial Rho GTPase 1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1
SwissProt ID
Q2HJF8 (Bovin), Q5ZM73 (Chick), Q6NVC5 (Danre), Q86UB0 (Human), Q8BG51 (Mouse), Q8IXI2 (Human), Q9D2R1 (Mouse)
Ncbi ID
9606, NP_060777, NP_001028738, NM_001033568, NP_001028738.1, NM_021536, NM_001163354, NM_001163355, NM_001033566
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of RHOT1 antibody
- Kit Component:
- - KN209190G1, RHOT1 gRNA vector 1 in pCas-Guide vector
- KN209190G2, RHOT1 gRNA vector 2 in pCas-Guide vector
- KN209190D, donor vector containing left and right homologous arms and GFP-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Fransson,S., (2006) Biochem. Biophys. Res. Commun. 344 (2), 500-510
RHOT1 monoclonal antibody (M01), clone 4H4 Western Blot analysis of RHOT1 expression in HeLa NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged RHOT1 is approximately 0.3ng/ml as a capture antibody.
Human Muscle; Rabbit Anti-RHOT1 Antibody. Catalog Number: ARP44817. Paraffin Embedded Tissue: Human Muscle. Cellular Data: Skeletal muscle cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; RHOT1 antibody - N-terminal region (ARP44817_P050) in Human Muscle cells using Immunohistochemistry
Human Fetal Lung; Host: Rabbit. Target Name: RHOT1. Sample Tissue: Fetal Lung lysates. Antibody Dilution: 1.0ug/ml.; RHOT1 antibody - N-terminal region (ARP44817_P050) in Human Fetal Lung cells using Western Blot
mouse brain and human neuroblastoma; RHOT1 antibody - N-terminal region (ARP44817_P050) in mouse brain and human neuroblastoma cells using Western Blot
Western Blot analysis of RHOT1 expression in transfected 293T cell line (H00055288-T01) by RHOT1 MaxPab polyclonal antibody.Lane 1: RHOT1 transfected lysate(72.49 KDa).Lane 2: Non-transfected lysate.
Human HeLa; WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; RHOT1 antibody - middle region (ARP44819_P050) in Human HeLa cells using Western Blot
Human HEK293T; WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. RHOT1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; RHOT1 antibody - N-terminal region (ARP44818_P050) in Human HEK293T cells using Western Blot
WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate
Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 ug/mL.
Immunohistochemistry of RHOT in mouse brain tissue with RHOT antibody at 5 ug/mL.
Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 ug/mL.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA335520 | RHOT1 antibody |
||||||
| Rabbit Polyclonal Anti-RHOT1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, Ms, Por, Rb, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA349118 | RHOT1 antibody |
||||||
| Rabbit Polyclonal Anti-RHOT1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN209190 | RHOT1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN209190BN | RHOT1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY422394 | RHOT1 overexpression lysate |
|
|
0.1 mg /
€480.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC216260L2V | Lenti ORF particles, RHOT1 (mGFP-tagged)-Human ras homolog gene family, member T1 (RHOT1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,330.00
|
|
| OriGene Technologies, Inc. | ||
| RC214409L1V | Lenti ORF particles, RHOT1 (Myc-DDK tagged) - Human ras homolog gene family, member T1 (RHOT1), transcript variant 1 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,400.00
|
|
| OriGene Technologies, Inc. | ||
| RC216260L1V | Lenti ORF particles, RHOT1 (Myc-DDK-tagged)-Human ras homolog gene family, member T1 (RHOT1), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,190.00
|
|
| OriGene Technologies, Inc. | ||
