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RHOT1 antibody

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Principal name

RHOT1 antibody

Alternative names for RHOT1 antibody

MIRO-1, ARHT1, Mitochondrial Rho GTPase 1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1

SwissProt ID

Q2HJF8 (Bovin), Q5ZM73 (Chick), Q6NVC5 (Danre), Q86UB0 (Human), Q8BG51 (Mouse), Q8IXI2 (Human), Q9D2R1 (Mouse)

Gene ID

55288 (RHOT1)

Ncbi ID

9606, NP_060777, NP_001028738, NP_001028738.1, NM_001033568

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of RHOT1 antibody

The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kise 1 (PINK1), form a complex that is involved in axol transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).

General readings

Fransson,S., (2006) Biochem. Biophys. Res. Commun. 344 (2), 500-510

H00055288-M01 - RHOT1
RHOT1 monoclonal antibody (M01), clone 4H4 Western Blot analysis of RHOT1 expression in HeLa NE ( Cat # L013V3 ).
H00055288-M01 - RHOT1
Detection limit for recombinant GST tagged RHOT1 is approximately 0.3ng/ml as a capture antibody.
ARP44817_P050 - RHOT1
Human Muscle; Rabbit Anti-RHOT1 Antibody. Catalog Number: ARP44817. Paraffin Embedded Tissue: Human Muscle. Cellular Data: Skeletal muscle cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; RHOT1 antibody - N-terminal region (ARP44817_P050) in Human Muscle cells using Immunohistochemistry
ARP44817_P050 - RHOT1
Human Fetal Lung; Host: Rabbit. Target Name: RHOT1. Sample Tissue: Fetal Lung lysates. Antibody Dilution: 1.0ug/ml.; RHOT1 antibody - N-terminal region (ARP44817_P050) in Human Fetal Lung cells using Western Blot
ARP44817_P050 - RHOT1
mouse brain and human neuroblastoma; RHOT1 antibody - N-terminal region (ARP44817_P050) in mouse brain and human neuroblastoma cells using Western Blot
H00055288-B01P - RHOT1
Western Blot analysis of RHOT1 expression in transfected 293T cell line (H00055288-T01) by RHOT1 MaxPab polyclonal antibody.Lane 1: RHOT1 transfected lysate(72.49 KDa).Lane 2: Non-transfected lysate.
ARP44819_P050 - RHOT1
Human HeLa; WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; RHOT1 antibody - middle region (ARP44819_P050) in Human HeLa cells using Western Blot
ARP44818_P050 - RHOT1
Human HEK293T; WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. RHOT1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; RHOT1 antibody - N-terminal region (ARP44818_P050) in Human HEK293T cells using Western Blot
NBP1-59021 - RHOT1
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-59021] - Human skeletal muscle cells (indicated with arrows).
NBP1-59021 - RHOT1
Western Blot: RHOT1 Antibody [NBP1-59021] - This Anti-RHOT1 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.25ug/ml.
NBP1-59022 - RHOT1
Western Blot: RHOT1 Antibody [NBP1-59022] - This Anti-RHOT1 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml.
NBP1-59609 - RHOT1
Western Blot: RHOT1 Antibody [NBP1-59609] - This Anti-RHOT1 antibody was used in Western Blot of 293T tissue lysate at a concentration of 1ug/ml.
NBP1-89011 - RHOT1
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in myocytes.
NBP1-89011 - RHOT1
Western Blot: Rhot1 Antibody [NBP1-89011] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
GTX46623 - RHOT1
Detection of RHOT1 in Hela using anti-RHOT1 antibody (GTX46623) at 1 µg/mL.
GTX46624 - RHOT1
Detection of RHOT1 in 293T using anti-RHOT1 antibody (GTX46624) at 1 µg/mL.
GTX46625 - RHOT1
Immunohistochemistry of human skeletal muscle cells using RHOT1 antibody (GTX46625)
GTX46625 - RHOT1
Detection of RHOT1 in HepG2 using anti-RHOT1 antibody (GTX46625) at 0.25 µg/mL.
GTX46625 - RHOT1
Western blot of mouse brain(left) and human neuroblastoma (right) using RHOT1 antibody (GTX46625)

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RHOT1 antibody
WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

RHOT1 antibody
Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+2 additional images

RHOT1 antibody
RHOT1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RHOT1 antibody
Western Blot analysis of RHOT1 expression in transfected 293T cell line (H00055288-T01) by RHOT1 MaxPab polyclonal antibody. Lane 1: RHOT1 transfected lysate(72.49 KDa). Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RHOT1 antibody
RHOT1 monoclonal antibody (M01), clone 4H4 Western Blot analysis of RHOT1 expression in HeLa NE ( Cat # L013V3 ). Mouse IgG1 4H4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.
+1 additional image

RHOT1 antibody
RHOT1 Mouse IgG1 1A12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RHOT1 antibody
Human Muscle; Rabbit Anti-RHOT1 Antibody. Catalog Number: ARP44817. Paraffin Embedded Tissue: Human Muscle. Cellular Data: Skeletal muscle cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; RHOT1 antibody - N-terminal region (ARP44817_P050) in Human Muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology
+2 additional images

RHOT1 antibody
Human HEK293T; WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. RHOT1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; RHOT1 antibody - N-terminal region (ARP44818_P050) in Human HEK293T cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

RHOT1 antibody
Human HeLa; WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; RHOT1 antibody - middle region (ARP44819_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

RHOT1 antibody
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-59021] - Human skeletal muscle cells (indicated with arrows). Rabbit Aff - Purified Bov, Can, Chk, Dros, Hu, Ms, Por, Rt, Xen, Ze P, WB
50 µg / €440.00
  Novus Biologicals Inc.
+1 additional image

RHOT1 antibody
Western Blot: RHOT1 Antibody [NBP1-59022] - This Anti-RHOT1 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

RHOT1 antibody
Western Blot: RHOT1 Antibody [NBP1-59609] - This Anti-RHOT1 antibody was used in Western Blot of 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

RHOT1 antibody
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RHOT1
RHOT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RHOT1
RHOT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RHOT1
RHOT1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RHOT1
RHOT1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RHOT1 293T Cell Transient Overexpression Lysate(Denatured)
RHOT1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

RHOT1 overexpression lysate
RHOT1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

19 Item(s)

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19 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (2)
  2. Primary Antibody (13)
  3. Protein | Growth Factor (4)
Clonality
  1. Polyclonal (11)
  2. Monoclonal (2)
Target
  1. RHOT1 (17)
Reactivity
  1. Human (Hu) (12)
  2. Mouse (Ms) (8)
  3. Rat (Rt) (8)
  4. Bovine (Bov) (5)
  5. Chicken (Chk) (4)
  6. Zebrafish (Ze) (4)
  7. Canine (Can) (3)
  8. Porcine (Por) (3)
  9. African clawed frog (2)
  10. Drosophila (Dros) (2)
  11. Equine (Eq) (1)
  12. Rabbit (Rb) (1)
  13. Xen (1)
Applications
  1. Western blot / Immunoblot (WB) (13)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (3)
  4. ELISA (detection) (E(detection)) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
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  2. Purified (7)
  3. Serum (1)
Host
  1. Rabbit (9)
  2. Mouse (4)
Images
  1. 1 image (11)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (11)
  2. IHC (2)
  3. Paraffin Sections (P) (2)
  4. ELISA (capture) (E(capture)) (1)
  5. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (4)
Source
  1. in vitro transl. (2)
Manufacturer
  1. Abnova Taiwan Corp. (9)
  2. Novus Biologicals Inc. (4)
  3. AVIVA Systems Biology (3)
  4. Acris - OriGene (3)
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