RIC8A antibody

Principal name

RIC8A antibody

Alternative names for RIC8A antibody

Ric-8A, RIC8, Synembryn-A, Resistance to inhibitors of cholinesterase 8 homolog A

SwissProt ID

Q3TIR3 (Mouse), Q45TX8 (Xenla), Q5E9J8 (Bovin), Q5ZL77 (Chick), Q642H7 (Danre), Q80ZG1 (Rat), Q9H9E7 (Human), Q9NPQ8 (Human), Q9NPQ8-3

Gene ID

60626 (RIC8A), 101489 (Ric8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Orang-Utan, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of RIC8A antibody

Resistance to inhibitors of cholinesterase (Ric) 8A is a guanine nucleotide exchange factor that activates certain G protein alpha-subunits. Earlier genetic studies have put RIC-8 under uncharacterized G protein signaling pathway that regulates centrosome movements during cell division. Various components of this pathway include G protein subunits of the Galphai class, GPR or GoLoco domain-containing proteins, RGS (regulator of G protein signaling) proteins, and other accessory factors. These proteins interact to regulate microtubule pulling forces during mitotic movement of chromosomes. The nucleus and the mitochondria of eukaryotic cells, as well as the chloroplasts in plants, contain their own genome that encodes a range of proteins and nucleic acids. The mitochondrial tRNA is encoded by nucleus and is transported in to mitochondria in many species with the help of mitochondrial transport complex as described (1). RIC8A is a 21-kDa tRNA-binding component of the tRNA complex that is partly homologous to subunit UCR6b of ubiquinol-cytochrome c reductase (OX PHOS complex III). RIC8A has binding specificity for the so-called type II tRNAs which, in vitro, require stimulation by a second group of tRNAs (type I) for import across the inner mitochondrial membrane; type I tRNAs, on the other hand, are imported efficiently by themselves, but this import is inhibited by type II tRNAs. Knockdown of RIC8A leads to deficiency of mitochondrial type II and elevation of type I tRNAs in vivo (2). In the presence of ATP, both types of tRNA are transferred from their respective receptors to a third subunit, RIC9, and thence through the membrane (3).
Ric8A also interacts with Adenylyl cyclase 5A to regulate its activity. The N-terminus of each individual AC usually has a unique amino acid sequence that is important for isoform-specific regulation (3). Several interacting proteins [e.g. snapin and PP2A (protein phosphatase 2A)] which bind the N-terminus of different ACs have been reported (3), suggesting that the N-terminus might function as a scaffold which selectively mediates the cross-talk between the cAMP signaling and other machinery. AC5 contains a very long N-terminus (242 amino acids) that interacts with Ric8a as binding partner of the N-terminus of AC5. Ric8a has been demonstrated to associate with many Gα proteins in mammalian cells, including Gαs, Gαq, Gαo, Gαi and Gαi3 (4). The association of Ric8A with AC5 N-terminal region suppress the AC5 activity via a Galfai-dependent pathway. Ric-8A interacts with GDP-bound Galpha proteins, stimulates release of GDP, and forms a stable nucleotide-free transition state complex with the Galpha protein; this complex dissociates upon binding of GTP to Galpha (4).

General readings

Klattenhoff C, Montecino M, Soto X, Guzman L, Romo X, Garcia MA, Mellstrom B, Naranjo JR, Hinrichs MV, Olate J. Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol. J Cell Physiol. 2003 May;195(2):151-7.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RIC8A antibody

  anti-RIC8A (RIC8) mouse monoclonal antibody, clone OTI3G3 (formerly 3G3)  
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-RIC8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501055, Dilution 1:50) Mouse IgG1 OTI3G3 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RIC8A (C-term) antibody

  Goat Polyclonal Antibody against RIC8A  
TA302971 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Chimp, Hu, Ms, Orang-Utan, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RIC8A antibody

  Rabbit Polyclonal antibody to RIC8A (resistance to inhibitors of cholinesterase 8 homolog A (C. elegans))  
Sample (30 ug of whole cell lysate). A: A431 . B: Hela. 7.5% SDS PAGE. TA309032 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RIC8A antibody

  Rabbit Polyclonal Anti-RIC8A Antibody - N-terminal region  
WB Suggested Anti-RIC8A Antibody Titration: 0.2-1 ug/ml; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

RIC8A - human gene knockout kit via CRISPR

RIC8A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RIC8A - human gene knockout kit via CRISPR

RIC8A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RIC8A - human gene knockout kit via CRISPR

RIC8A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RIC8A - human gene knockout kit via CRISPR

RIC8A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ric8 - mouse gene knockout kit via CRISPR

Ric8 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ric8 - mouse gene knockout kit via CRISPR

Ric8 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ric8 - mouse gene knockout kit via CRISPR

Ric8 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ric8 - mouse gene knockout kit via CRISPR

Ric8 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RIC8A

  Recombinant protein of human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A)  
RIC8A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RIC8A (mGFP-tagged) - Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A), 200 uL, >10^7 TU/mL

Lenti ORF particles, RIC8A (mGFP-tagged) - Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A), 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.

Lenti ORF particles, RIC8A (Myc-DDK tagged) - Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A) , 200 uL, >10^7 TU/mL

Lenti ORF particles, RIC8A (Myc-DDK tagged) - Human resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) (RIC8A) , 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ric8 (GFP-tagged) - Mouse resistance to inhibitors of cholinesterase 8 homolog (C. elegans) (Ric8), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ric8 (GFP-tagged) - Mouse resistance to inhibitors of cholinesterase 8 homolog (C. elegans) (Ric8), 200 uL, >10^7 TU/mL  
200 µl / €1,160.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ric8 (Myc-DDK-tagged) - Mouse resistance to inhibitors of cholinesterase 8 homolog (C. elegans) (Ric8), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ric8 (Myc-DDK-tagged) - Mouse resistance to inhibitors of cholinesterase 8 homolog (C. elegans) (Ric8), 200 uL, >10^7 TU/mL  
200 µl / €1,040.00
  OriGene Technologies, Inc.

17 Item(s)

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