RICTOR antibody

Principal name

RICTOR antibody

Alternative names for RICTOR antibody

KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3

SwissProt ID

Q6QI06 (Mouse), Q6R327 (Human)

Gene ID

253260 (RICTOR)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Prim (Primate)

Available hosts

Mouse, Goat, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of RICTOR antibody

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals . mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1 and rictor . The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro . This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes . This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR .

General readings

Sarbassov dos D, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptorindependent pathway that regulates the cytoskeleton. Curr Biol. 2004 Jul 27;14(14):1296-302.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RICTOR antibody

Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and Human breast carcinoma (right) using RICTOR antibody Cat.-No AM06499SU-N with DAB staining. Mouse IgG1 7B3 Ascites Hu, Mky, Ms E, F, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

RICTOR (1-99) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 1F3 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

RICTOR (C-term) antibody

TA303018 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Can, Hu, Ms P
0.1 mg / €325.00
  OriGene Technologies, Inc.

RICTOR antibody

Sample(30 ug whole cell lysate). A: A431 . 5% SDS PAGE. TA308477 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RICTOR antibody

Western blot analysis of Rictor with lysate from Jurkat cells (Lane 1) and Hela cells (Lane 2). Rabbit Purified Hu, Ms, Rt WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

RICTOR antibody

Western Blot: RICTOR Antibody (7B3) [TA336802] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates. Mouse IgG1 7B3 Purified Hu, Ms, Prim C, E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RICTOR antibody

Western blot analysis using RICTOR antibody Cat.-No AM06502SU-N against Hela (1), PANC-1 (2), MOLT4 (3), and HepG2 (4) cell lysate. Mouse IgG1 4H5 Ascites Hu, Mky, Ms E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

RICTOR (incl. pos. control) antibody

Detection of endogenous Rictor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to
SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20222PU-N RICTOR antibody (Clone 1G11) at 0.5 µg/ml for 1h at RT
and developed by ECL (exp. time: 30 sec).
Lane 1: HeLa
Lane 2: HepG2
Lane 3: HEK293
Lane 4: SH-SY5Y
Lane 5: MDCK
Lane 6: PC12
Lane 7: CMT 93 
Lane 8: Neuro 2A
Lane 9: 3T3 Mouse IgG1 1G11 Purified Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RICTOR (C-term) antibody

Figure 1. Staining RICTOR in Renal Tubular Epithelium by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Goat Aff - Purified Bear, Blood fluke, Bov, Bt, Can, Elephant, Eq, Hu, Mky, Ms, Op, Por, Prim, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

RICTOR (N-term) antibody

Formalin-fixed and paraffin-embedded human Lymph tissue reacted with Rictor antibody (N-term)(AP13961PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

RICTOR (N-term) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR antibody (N-term) (AP13964PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RICTOR antibody

Western Blot analysis of RICTOR expression in transfected 293T cell line (H00253260-T01) by RICTOR MaxPab polyclonal antibody.

Lane 1: RICTOR transfected lysate(29.15 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RICTOR antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between RICTOR and RPS6KB1 HeLa cells were stained with anti-RICTOR rabbit purified polyclonal 1:1200 and anti-RPS6KB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RICTOR antibody

Immunoperoxidase of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG2b 1F3 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RICTOR antibody

RICTOR Mouse IgG2b 1F3 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

RICTOR antibody

Formalin-fixed and paraffin-embedded human Lymph tissue reacted with RICTOR polyclonal antibody ( Cat # PAB2053 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

RICTOR antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR polyclonal antibody ( Cat # PAB2054 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

RICTOR antibody

RICTOR polyclonal antibody ( Cat # PAB6780 ) ( 2.5 µg/ml ) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu E, P
0.1 mg / €400.00
  Abnova Taiwan Corp.

RICTOR antibody

WB and IP: Rictor Antibody [NB100-611] - Detection of Human Rictoron HeLa whole cell lysate using NB100-611. Rictor was also IPed using rabbit anti-Rictor antibodies NB100-612 and another one. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

RICTOR antibody

Immunohistochemistry-Paraffin: RICTOR Antibody [NB100-612] - FFPE section of human prostate carcinoma  Affinity purified rabbit anti-Rictor used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu, Ms F, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

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